Advertisement
MCP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/mcp.M100025-MCP200 on February 21, 2002.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M100025-MCP200v1
1/3/243    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Glossary
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fransen, M.
Right arrow Articles by Van Veldhoven, P. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fransen, M.
Right arrow Articles by Van Veldhoven, P. P.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 1:243-252, 2002.
© 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


Research

Analysis of Mammalian Peroxin Interactions Using a Non-transcription-based Bacterial Two-hybrid Assay*

Marc Fransen{ddagger},§, Chantal Brees{ddagger}, Karen Ghys{ddagger}, Leen Amery{ddagger}, Guy P. Mannaerts{ddagger}, Daniel Ladant|| and Paul P. Van Veldhoven{ddagger},**

{ddagger} Departement Moleculaire Celbiologie, Afdeling Farmacologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, Herestraat 49 (O/N), B-3000 Leuven, Belgium
|| Département de Biochimie et Génétique Moleculaire, Institut Pasteur, Unité de Biochemie Cellulaire (CNRS URA 2185), 28 rue du Dr. Roux, 75724 Paris CEDEX 15, France

In recent years, substantial progress has been made in the identification of proteins involved in peroxisome biogenesis. However, with the exception of the peroxisome-targeting signal receptors and the receptor docking proteins, the function of most of these proteins, called peroxins, remains largely unknown. One step toward elucidating the function of a protein is to identify its interacting partners. We have used a non-transcription-based bacterial two-hybrid system to analyze the interactions among a set of 12 mammalian peroxins and a yeast protein three-hybrid system to investigate whether proteins that interact with the same peroxin and have overlapping binding sites cooperate or compete for this site. Here we report a detailed interaction map of these peroxins and demonstrate that (i) farnesylation, and not the CAAX motif, of Pex19p strongly enhances its affinity for Pex13p; (ii) the CAAXmotif, and not farnesylation, of Pex19p strongly enhances its affinity for Pex11pß; and (iii) the C3HC4 RING (really interesting new gene) finger domain of Pex12p does not alter the binding properties of Pex5p for the C-terminal peroxisome-targeting signal PTS1. Finally, we show that the Pex5p-Pex13p interaction is bridged by Pex14p and that the latter molecule exists predominantly as a dimer in vivo. Collectively, as demonstrated in this work with peroxins, these results indicate that the bacterial two-hybrid system is an attractive complementary approach to the conventional transcription-based yeast two-hybrid system for studying protein-protein interactions.


** To whom correspondence may be addressed. Tel.: 32-16-345802; Fax: 32-16-345699; E-mail: paul.vanveldhoven{at}med.kuleuven.ac.be.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
J. Biol. Chem.Home page
C. P. Grou, A. F. Carvalho, M. P. Pinto, S. J. Huybrechts, C. Sa-Miranda, M. Fransen, and J. E. Azevedo
Properties of the Ubiquitin-Pex5p Thiol Ester Conjugate
J. Biol. Chem., April 17, 2009; 284(16): 10504 - 10513.
[Abstract] [Full Text] [PDF]


Home page
Protein Eng Des SelHome page
P. Secco, E. D'Agostini, R. Marzari, M. Licciulli, R. Di Niro, S. D'Angelo, A. R.M. Bradbury, U. Dianzani, C. Santoro, and D. Sblattero
Antibody library selection by the {beta}-lactamase protein fragment complementation assay
Protein Eng. Des. Sel., March 1, 2009; 22(3): 149 - 158.
[Abstract] [Full Text] [PDF]


Home page
BMJ Case ReportsHome page
S. J Huybrechts, P. P Van Veldhoven, I. Hoffman, R. Zeevaert, R. de Vos, P. Demaerel, M. Brams, J. Jaeken, M. Fransen, and D. Cassiman
Identification of a novel PEX14 mutation in Zellweger syndrome
BMJ Case Reports, January 22, 2009; 2009(jan21_1): bcr0720080503 - bcr0720080503.
[Abstract] [Full Text]


Home page
J. Biol. Chem.Home page
N. Cyr, K. P. Madrid, R. Strasser, M. Aurousseau, R. Finn, J. Ausio, and A. Jardim
Leishmania donovani Peroxin 14 Undergoes a Marked Conformational Change following Association with Peroxin 5
J. Biol. Chem., November 14, 2008; 283(46): 31488 - 31499.
[Abstract] [Full Text] [PDF]


Home page
J. Med. Genet.Home page
S J Huybrechts, P P Van Veldhoven, I Hoffman, R Zeevaert, R de Vos, P Demaerel, M Brams, J Jaeken, M Fransen, and D Cassiman
Identification of a novel PEX14 mutation in Zellweger syndrome
J. Med. Genet., June 1, 2008; 45(6): 376 - 383.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
C. P. Grou, A. F. Carvalho, M. P. Pinto, S. Wiese, H. Piechura, H. E. Meyer, B. Warscheid, C. Sa-Miranda, and J. E. Azevedo
Members of the E2D (UbcH5) Family Mediate the Ubiquitination of the Conserved Cysteine of Pex5p, the Peroxisomal Import Receptor
J. Biol. Chem., May 23, 2008; 283(21): 14190 - 14197.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
A. F. Carvalho, M. P. Pinto, C. P. Grou, I. S. Alencastre, M. Fransen, C. Sa-Miranda, and J. E. Azevedo
Ubiquitination of Mammalian Pex5p, the Peroxisomal Import Receptor
J. Biol. Chem., October 26, 2007; 282(43): 31267 - 31272.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
Y. Matsuzono, T. Matsuzaki, and Y. Fujiki
Functional domain mapping of peroxin Pex19p: interaction with Pex3p is essential for function and translocation
J. Cell Sci., September 1, 2006; 119(17): 3539 - 3550.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J. Costa-Rodrigues, A. F. Carvalho, M. Fransen, E. Hambruch, W. Schliebs, C. Sa-Miranda, and J. E. Azevedo
Pex5p, the Peroxisomal Cycling Receptor, Is a Monomeric Non-globular Protein
J. Biol. Chem., July 1, 2005; 280(26): 24404 - 24411.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
A. Halbach, S. Lorenzen, C. Landgraf, R. Volkmer-Engert, R. Erdmann, and H. Rottensteiner
Function of the PEX19-binding Site of Human Adrenoleukodystrophy Protein as Targeting Motif in Man and Yeast: PMP TARGETING IS EVOLUTIONARILY CONSERVED
J. Biol. Chem., June 3, 2005; 280(22): 21176 - 21182.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
K. Shiozawa, N. Maita, K. Tomii, A. Seto, N. Goda, Y. Akiyama, T. Shimizu, M. Shirakawa, and H. Hiroaki
Structure of the N-terminal Domain of PEX1 AAA-ATPase: CHARACTERIZATION OF A PUTATIVE ADAPTOR-BINDING DOMAIN
J. Biol. Chem., November 26, 2004; 279(48): 50060 - 50068.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J. Costa-Rodrigues, A. F. Carvalho, A. M. Gouveia, M. Fransen, C. Sa-Miranda, and J. E. Azevedo
The N Terminus of the Peroxisomal Cycling Receptor, Pex5p, Is Required for Redirecting the Peroxisome-associated Peroxin Back to the Cytosol
J. Biol. Chem., November 5, 2004; 279(45): 46573 - 46579.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
A. Schafer, D. Kerssen, M. Veenhuis, W.-H. Kunau, and W. Schliebs
Functional Similarity between the Peroxisomal PTS2 Receptor Binding Protein Pex18p and the N-Terminal Half of the PTS1 Receptor Pex5p
Mol. Cell. Biol., October 15, 2004; 24(20): 8895 - 8906.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. Fransen, I. Vastiau, C. Brees, V. Brys, G. P. Mannaerts, and P. P. Van Veldhoven
Potential Role for Pex19p in Assembly of PTS-Receptor Docking Complexes
J. Biol. Chem., March 26, 2004; 279(13): 12615 - 12624.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
M. E. Oliveira, A. M. Gouveia, R. A. Pinto, C. Sa-Miranda, and J. E. Azevedo
The Energetics of Pex5p-mediated Peroxisomal Protein Import
J. Biol. Chem., October 10, 2003; 278(41): 39483 - 39488.
[Abstract] [Full Text] [PDF]


Home page
J. Cell Sci.Home page
J. H. Eckert and N. Johnsson
Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome
J. Cell Sci., September 1, 2003; 116(17): 3623 - 3634.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
A. M. Gouveia, C. P. Guimaraes, M. E. Oliveira, C. Reguenga, C. Sa-Miranda, and J. E. Azevedo
Characterization of the Peroxisomal Cycling Receptor, Pex5p, Using a Cell-free in Vitro Import System
J. Biol. Chem., January 3, 2003; 278(1): 226 - 232.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement