| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Molecular & Cellular Proteomics 1:293-303, 2002.
© 2002 by The American Society for Biochemistry and Molecular Biology, Inc.
,
,||,**
Cole Eye Institute
|| Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195
¶ Department of Biochemistry and Molecular Biology, Wright State University, Dayton, Ohio 45435
As part of ongoing efforts to better understand the role of protein oxidative modifications in retinal pathology, protein nitration in retina has been compared between rats exposed to damaging light or maintained in the dark. In the course of the research, Western methodology for detecting nitrotyrosine-containing proteins has been improved by incorporating chemical reduction of nitrotyrosine to aminotyrosine, allowing specific and nonspecific nitrotyrosine immunoreactivity to be distinguished. A liquid chromatography MS/MS detection strategy was used that selects all possible nitrotyrosine peptides for MS/MS based on knowing the protein identity. Quantitative liquid chromatography MS/MS analyses with tetranitromethane-modified albumin demonstrated the approach capable of identifying sites of tyrosine nitration with detection limits of 4–33 fmol. Using two-dimensional gel electrophoresis, Western detection, and mass spectrometric analyses, several different nitrotyrosine-immunoreactive proteins were identified in light-exposed rat retina compared with those maintained in the dark. Immunocytochemical analyses of retina revealed that rats reared in darkness exhibited more nitrotyrosine immunoreactivity in the photoreceptor outer segments. After intense light exposure, immunoreactivity decreased in the outer segments and increased in the photoreceptor inner segments and retinal pigment epithelium. These results suggest that light modulates retinal protein nitration in vivo and that nitration may participate in the biochemical sequela leading to light-induced photoreceptor cell death. Furthermore, the identification of nitrotyrosine-containing proteins from rats maintained in the dark, under non-pathological conditions, provides the first evidence of a possible role for protein nitration in normal retinal physiology.
To whom correspondence may be addressed: Cole Eye Inst., Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195. Tel.: 216-445-0424; Fax: 216-445-3670; E-mail: miyagim{at}ccf.org** To whom correspondence may be addressed: Cole Eye Inst. and Lerner Research Inst., Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195. Tel.: 216-445-0425; Fax: 216-445-3670; E-mail: crabbj{at}ccf.org
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Dhiman, E. S. Nakayasu, Y. H. Madaiah, B. K. Reynolds, J.-j. Wen, I. C. Almeida, and N. J. Garg Enhanced Nitrosative Stress during Trypanosoma cruzi Infection Causes Nitrotyrosine Modification of Host Proteins: Implications in Chagas' Disease Am. J. Pathol., September 1, 2008; 173(3): 728 - 740. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhan, Y. Du, J. S. Crabb, X. Gu, T. S. Kern, and J. W. Crabb Targets of Tyrosine Nitration in Diabetic Rat Retina Mol. Cell. Proteomics, May 1, 2008; 7(5): 864 - 874. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Ethen, C. Reilly, X. Feng, T. W. Olsen, and D. A. Ferrington Age-Related Macular Degeneration and Retinal Protein Modification by 4-Hydroxy-2-nonenal Invest. Ophthalmol. Vis. Sci., August 1, 2007; 48(8): 3469 - 3479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sun, S. C. Finnemann, M. Febbraio, L. Shan, S. P. Annangudi, E. A. Podrez, G. Hoppe, R. Darrow, D. T. Organisciak, R. G. Salomon, et al. Light-induced Oxidation of Photoreceptor Outer Segment Phospholipids Generates Ligands for CD36-mediated Phagocytosis by Retinal Pigment Epithelium: A POTENTIAL MECHANISM FOR MODULATING OUTER SEGMENT PHAGOCYTOSIS UNDER OXIDANT STRESS CONDITIONS J. Biol. Chem., February 17, 2006; 281(7): 4222 - 4230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Tanito, M. H. Elliott, Y. Kotake, and R. E. Anderson Protein Modifications by 4-Hydroxynonenal and 4-Hydroxyhexenal in Light-Exposed Rat Retina Invest. Ophthalmol. Vis. Sci., October 1, 2005; 46(10): 3859 - 3868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Shu, A.M. Fry, B. Tulloch, F.D.C. Manson, J.W. Crabb, H. Khanna, A.J. Faragher, A. Lennon, S. He, P. Trojan, et al. RPGR ORF15 isoform co-localizes with RPGRIP1 at centrioles and basal bodies and interacts with nucleophosmin Hum. Mol. Genet., May 1, 2005; 14(9): 1183 - 1197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Casoni, M. Basso, T. Massignan, E. Gianazza, C. Cheroni, M. Salmona, C. Bendotti, and V. Bonetto Protein Nitration in a Mouse Model of Familial Amyotrophic Lateral Sclerosis: POSSIBLE MULTIFUNCTIONAL ROLE IN THE PATHOGENESIS J. Biol. Chem., April 22, 2005; 280(16): 16295 - 16304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wu, A. Hasan, T. Liu, D. C. Teller, and J. W. Crabb Identification of CRALBP Ligand Interactions by Photoaffinity Labeling, Hydrogen/Deuterium Exchange, and Structural Modeling J. Biol. Chem., June 25, 2004; 279(26): 27357 - 27364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Koeck, B. Levison, S. L. Hazen, J. W. Crabb, D. J. Stuehr, and K. S. Aulak Tyrosine Nitration Impairs Mammalian Aldolase A Activity Mol. Cell. Proteomics, June 1, 2004; 3(6): 548 - 557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-Y. Zhu, M. Rodriguez-Porcel, M. D. Bentley, A. R. Chade, V. Sica, C. Napoli, N. Caplice, E. L. Ritman, A. Lerman, and L. O. Lerman Antioxidant Intervention Attenuates Myocardial Neovascularization in Hypercholesterolemia Circulation, May 4, 2004; 109(17): 2109 - 2115. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Keightley, L. Shang, and M. Kinter Proteomic Analysis of Oxidative Stress-resistant Cells: A SPECIFIC ROLE FOR ALDOSE REDUCTASE OVEREXPRESSION IN CYTOPROTECTION Mol. Cell. Proteomics, February 1, 2004; 3(2): 167 - 175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S. Aulak, T. Koeck, J. W. Crabb, and D. J. Stuehr Dynamics of protein nitration in cells and mitochondria Am J Physiol Heart Circ Physiol, January 1, 2004; 286(1): H30 - H38. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Gu, S. G. Meer, M. Miyagi, M. E. Rayborn, J. G. Hollyfield, J. W. Crabb, and R. G. Salomon Carboxyethylpyrrole Protein Adducts and Autoantibodies, Biomarkers for Age-related Macular Degeneration J. Biol. Chem., October 24, 2003; 278(43): 42027 - 42035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Wu, Y. Yang, N. Shaw, S. Bhattacharya, L. Yan, K. West, K. Roth, N. Noy, J. Qin, and J. W. Crabb Mapping the Ligand Binding Pocket in the Cellular Retinaldehyde Binding Protein J. Biol. Chem., March 28, 2003; 278(14): 12390 - 12396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Golovleva, S. Bhattacharya, Z. Wu, N. Shaw, Y. Yang, K. Andrabi, K. A. West, M. S. I. Burstedt, K. Forsman, G. Holmgren, et al. Disease-causing Mutations in the Cellular Retinaldehyde Binding Protein Tighten and Abolish Ligand Interactions J. Biol. Chem., March 28, 2003; 278(14): 12397 - 12402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. West, L. Yan, K. Shadrach, J. Sun, A. Hasan, M. Miyagi, J. S. Crabb, J. G. Hollyfield, A. D. Marmorstein, and J. W. Crabb Protein Database, Human Retinal Pigment Epithelium Mol. Cell. Proteomics, January 1, 2003; 2(1): 37 - 49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Crabb, M. Miyagi, X. Gu, K. Shadrach, K. A. West, H. Sakaguchi, M. Kamei, A. Hasan, L. Yan, M. E. Rayborn, et al. From the Cover: Drusen proteome analysis: An approach to the etiology of age-related macular degeneration PNAS, November 12, 2002; 99(23): 14682 - 14687. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Journal of Lipid Research | ASBMB Today |
