MCP Agilent Technologies
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M100036-MCP200 on May 21, 2002.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M100036-MCP200v1
1/6/421    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Edmondson, R. D.
Right arrow Articles by Ping, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Edmondson, R. D.
Right arrow Articles by Ping, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 1:421-433, 2002.
© 2002 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Protein Kinase C {epsilon} Signaling Complexes Include Metabolism- and Transcription/Translation-related Proteins

Complimentary Separation Techniques With LC/MS/MS*,S

Ricky D. Edmondson{ddagger}, Thomas M. Vondriska§,¶, Kelli J. Biederman{ddagger}, Jun Zhang§,¶, Richard C. Jones{ddagger}, Yuting Zheng§,¶, David L. Allen||, Joanne X. Xiu§,¶, Ernest M. Cardwell§,¶, Michael R. Pisano{ddagger} and Peipei Ping§,¶,**

§ Department of Physiology and Biophysics, Division of Cardiology, University of Louisville, Louisville, Kentucky 40202
Department of Medicine, Division of Cardiology, University of Louisville, Louisville, Kentucky 40202
{ddagger} Proteomic Research Services, Inc., Ann Arbor, Michigan 48108
|| Department of Ophthalmology, Kellogg Eye Center, University of Michigan, Ann Arbor, Michigan 48108

The serine/threonine kinase protein kinase C {epsilon} (PKC{epsilon}) has been shown to be a critical component in the heart’s resistance to cell death following ischemic insult. Recent studies have indicated that PKC{epsilon} forms multi-protein signaling complexes to accomplish signal transduction in cardiac protection. Using two-dimensional electrophoresis (2DE), combined with matrix-assisted laser desorption ionization mass spectrometry (MS), the initial analysis of these complexes identified signaling molecules, structural proteins, and stress-activated proteins. The initial analysis, although fruitful, was limited by the number of proteins revealed on the 2D gels. It was also apparent that many known cardiac protective functions of PKC{epsilon} could not be fully accounted for by the proteins identified in the initial analysis. Here we reported the identification of an additional 57 proteins in PKC{epsilon} complexes using complimentary separation techniques, combined with high sensitivity MS. These techniques include 2DE or large format 1D SDS-PAGE followed by LC/MS/MS and solution trypsin digestion followed by LC/MS/MS, all of which yielded novel data regarding PKC{epsilon} protein complexes. Nanoscale LC/MS/MS for the analysis of gel-isolated proteins was performed with sub-femtomole sensitivity. In contrast to 2DE analyses, the identification of proteins from 1D gels was independent of their visualization via staining and allowed for the identification of proteins with high isoelectric points. We found that PKC{epsilon} complexes contain numerous structural and signaling molecules that had escaped detection by our previous analyses. Most importantly, we identified two new groups of proteins that were previously unrecognized as components of the PKC{epsilon} complex: metabolism-related proteins and transcription/translation-related proteins.

** To whom correspondence should be addressed: Cardiology Research, Suite 122, Baxter Biomedical Research Bldg., 570 S. Preston St., Louisville, KY 40202-1783. Tel.: 502-852-8431; Fax: 502-852-8421; E-mail: ping{at}ntr.net


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. C. Wu and R. J. Solaro
Protein Kinase C {zeta}: A NOVEL REGULATOR OF BOTH PHOSPHORYLATION AND DE-PHOSPHORYLATION OF CARDIAC SARCOMERIC PROTEINS
J. Biol. Chem., October 19, 2007; 282(42): 30691 - 30698.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
M. H. Aziz, H. T. Manoharan, D. R. Church, N. E. Dreckschmidt, W. Zhong, T. D. Oberley, G. Wilding, and A. K. Verma
Protein Kinase C{varepsilon} Interacts with Signal Transducers and Activators of Transcription 3 (Stat3), Phosphorylates Stat3Ser727, and Regulates Its Constitutive Activation in Prostate Cancer
Cancer Res., September 15, 2007; 67(18): 8828 - 8838.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. Bjarnadottir, D. L. Misner, S. Haverfield-Gross, S. Bruun, V. G. Helgason, H. Stefansson, A. Sigmundsson, D. R. Firth, B. Nielsen, R. Stefansdottir, et al.
Neuregulin1 (NRG1) Signaling through Fyn Modulates NMDA Receptor Phosphorylation: Differential Synaptic Function in NRG1+/- Knock-Outs Compared with Wild-Type Mice
J. Neurosci., April 25, 2007; 27(17): 4519 - 4529.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
J. Peng, K. Raddatz, J. D. Molkentin, Y. Wu, S. Labeit, H. Granzier, and M. Gotthardt
Cardiac Hypertrophy and Reduced Contractility in Hearts Deficient in the Titin Kinase Region
Circulation, February 13, 2007; 115(6): 743 - 751.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
J. N. Weiss, L. Yang, and Z. Qu
Thematic review series: Systems Biology Approaches to Metabolic and Cardiovascular Disorders. Network perspectives of cardiovascular metabolism
J. Lipid Res., November 1, 2006; 47(11): 2355 - 2366.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
K. Inagaki, E. Churchill, and D. Mochly-Rosen
Epsilon protein kinase C as a potential therapeutic target for the ischemic heart
Cardiovasc Res, May 1, 2006; 70(2): 222 - 230.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
E. McGregor and M. J. Dunn
Proteomics of the Heart: Unraveling Disease
Circ. Res., February 17, 2006; 98(3): 309 - 321.
[Abstract] [Full Text] [PDF]

Home page
 Exp PhysiolHome page
T. De Celle, F. Vanrobaeys, P. Lijnen, W. M. Blankesteijn, S. Heeneman, J. Van Beeumen, B. Devreese, J. F. M Smits, and B. J. A Janssen
Alterations in mouse cardiac proteome after in vivo myocardial infarction: permanent ischaemia versus ischaemia-reperfusion
Exp Physiol, July 1, 2005; 90(4): 593 - 606.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
M. Juhaszova, C. Rabuel, D. B. Zorov, E. G. Lakatta, and S. J. Sollott
Protection in the aged heart: preventing the heart-break of old age?
Cardiovasc Res, May 1, 2005; 66(2): 233 - 244.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
Z.-Q. Jin, E. J. Goetzl, and J. S. Karliner
Sphingosine Kinase Activation Mediates Ischemic Preconditioning in Murine Heart
Circulation, October 5, 2004; 110(14): 1980 - 1989.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
P. H. Goldspink, D. E. Montgomery, L. A. Walker, D. Urboniene, R. D. McKinney, D. L. Geenen, R. J. Solaro, and P. M. Buttrick
Protein Kinase C{epsilon} Overexpression Alters Myofilament Properties and Composition During the Progression of Heart Failure
Circ. Res., August 20, 2004; 95(4): 424 - 432.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
G. K. Kumar and J. B. Klein
Analysis of expression and posttranslational modification of proteins during hypoxia
J Appl Physiol, March 1, 2004; 96(3): 1178 - 1186.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
P. Ping
Identification of Novel Signaling Complexes by Functional Proteomics
Circ. Res., October 3, 2003; 93(7): 595 - 603.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. Zhang, P. Ping, T. M. Vondriska, X.-L. Tang, G.-W. Wang, E. M. Cardwell, and R. Bolli
Cardioprotection involves activation of NF-{kappa}B via PKC-dependent tyrosine and serine phosphorylation of I{kappa}B-{alpha}
Am J Physiol Heart Circ Physiol, October 1, 2003; 285(4): H1753 - H1758.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J. N. Weiss, P. Korge, H. M. Honda, and P. Ping
Role of the Mitochondrial Permeability Transition in Myocardial Disease
Circ. Res., August 22, 2003; 93(4): 292 - 301.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
P. Ping
A new chapter in cardiac PKC signaling studies: searching for isoform-specific molecular targets. Focus on: "Isoenzyme-selective regulation of SERCA2 gene expression by protein kinase C in neonatal rat ventricular myocytes"
Am J Physiol Cell Physiol, July 1, 2003; 285(1): C19 - C21.
[Full Text] [PDF]

Home page
 Circ. Res.Home page
R. L. Winslow and M. S. Boguski
Genome Informatics: Current Status and Future Prospects
Circ. Res., May 16, 2003; 92(9): 953 - 961.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.