MCP AbD Serotec
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/mcp.M200027-MCP200 on May 30, 2002.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M200027-MCP200v1
1/6/434    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, L.
Right arrow Articles by Burlingame, A. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, L.
Right arrow Articles by Burlingame, A. L.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 1:434-450, 2002.
© 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


Research

The Identification of Protein-Protein Interactions of the Nuclear Pore Complex of Saccharomyces cerevisiae Using High Throughput Matrix-assisted Laser Desorption Ionization Time-of-Flight Tandem Mass Spectrometry*

Lan Huang{ddagger}, Michael A. Baldwin{ddagger}, David A. Maltby{ddagger}, Katalin F. Medzihradszky{ddagger}, Peter R. Baker{ddagger}, Nadia Allen§, Michael Rexach§, Ricky D. Edmondson, Jennifer Campbell||, Peter Juhasz||, Steven A. Martin||, Marvin L. Vestal|| and Alma L. Burlingame{ddagger},**

{ddagger} University of California, San Francisco, California 94143-0446
§ Stanford University, Stanford, California 94305
Genomic Solutions, Inc., Ann Arbor, Michigan 48108
|| Applied Biosystems, Framingham, Massachusetts 01701

Mass spectrometry has become the technology of choice for detailed identification of proteins in complex mixtures. Although electrophoretic separation, proteolytic digestion, mass spectrometric analysis of unseparated digests, and database searching have become standard methods in widespread use, peptide sequence information obtained by collision-induced dissociation and tandem mass spectrometry is required to establish the most comprehensive and reliable results. Most tandem mass spectrometers in current use employ electrospray ionization. In this work a novel tandem mass spectrometer employing matrix-assisted laser desorption ionization-time-of-flight/time-of-flight operating at 200 Hz has been used to identify proteins interacting with known nucleoporins in the nuclear pore complex of Saccharomyces cerevisiae. Proteins interacting with recombinant proteins as bait were purified from yeast extracts and then separated by one-dimensional SDS-PAGE. Although peptide mass fingerprinting is sometimes sufficient to identify proteins, this study shows the importance of employing tandem mass spectrometry for identifying proteins in mixtures or as covalently modified forms. The rules for incorporating these features into MS-Tag are presented. In addition to providing an evaluation of the sensitivity and overall quality of collision-induced dissociation spectra obtained, standard conditions for ionization and fragmentation have been selected that would allow automatic data collection and analysis, without the need to adjust parameters in a sample-specific fashion. Other considerations essential for successful high throughput protein analysis are discussed.


** To whom correspondence should be addressed. Tel.: 415-476-5641; Fax: 415-476-0688; E-mail: alb{at}itsa.ucsf.edu


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Mol. Cell. ProteomicsHome page
D. P. Denning and M. F. Rexach
Rapid Evolution Exposes the Boundaries of Domain Structure and Function in Natively Unfolded FG Nucleoporins
Mol. Cell. Proteomics, February 1, 2007; 6(2): 272 - 282.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. ProteomicsHome page
R. J. Chalkley, P. R. Baker, L. Huang, K. C. Hansen, N. P. Allen, M. Rexach, and A. L. Burlingame
Comprehensive Analysis of a Multidimensional Liquid Chromatography Mass Spectrometry Dataset Acquired on a Quadrupole Selecting, Quadrupole Collision Cell, Time-of-flight Mass Spectrometer: II. New Developments in Protein Prospector Allow for Reliable and Comprehensive Automatic Analysis of Large Datasets
Mol. Cell. Proteomics, August 1, 2005; 4(8): 1194 - 1204.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
W. Khovidhunkit, J. P. Hachem, K. F. Medzihradszky, P. N. Duchateau, J. K. Shigenaga, A. H. Moser, I. Movsesyan, J. Naya-Vigne, J. P. Kane, K. R. Feingold, et al.
Parotid secretory protein is an HDL-associated protein with anticandidal activity
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2005; 288(5): R1306 - R1315.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. Hirsch, K. C. Hansen, A. L. Burlingame, and M. A. Matthay
Proteomics: current techniques and potential applications to lung disease
Am J Physiol Lung Cell Mol Physiol, July 1, 2004; 287(1): L1 - L23.
[Abstract] [Full Text] [PDF]


Home page
Drug Metab. Dispos.Home page
S. Nisar, C. S. Lane, A. F. Wilderspin, K. J. Welham, W. J. Griffiths, and L. H. Patterson
A PROTEOMIC APPROACH TO THE IDENTIFICATION OF CYTOCHROME P450 ISOFORMS IN MALE AND FEMALE RAT LIVER BY NANOSCALE LIQUID CHROMATOGRAPHY-ELECTROSPRAY IONIZATION-TANDEM MASS SPECTROMETRY
Drug Metab. Dispos., April 1, 2004; 32(4): 382 - 386.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. ProteomicsHome page
M. A. Baldwin
Protein Identification by Mass Spectrometry: Issues to be Considered
Mol. Cell. Proteomics, January 1, 2004; 3(1): 1 - 9.
[Full Text] [PDF]


Home page
Mol. Cell. ProteomicsHome page
M. Knuesel, Y. Wan, Z. Xiao, E. Holinger, N. Lowe, W. Wang, and X. Liu
Identification of Novel Protein-Protein Interactions Using A Versatile Mammalian Tandem Affinity Purification Expression System
Mol. Cell. Proteomics, November 1, 2003; 2(11): 1225 - 1233.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
B. Pyhtila and M. Rexach
A Gradient of Affinity for the Karyopherin Kap95p along the Yeast Nuclear Pore Complex
J. Biol. Chem., October 24, 2003; 278(43): 42699 - 42709.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. ProteomicsHome page
K. C. Hansen, G. Schmitt-Ulms, R. J. Chalkley, J. Hirsch, M. A. Baldwin, and A. L. Burlingame
Mass Spectrometric Analysis of Protein Mixtures at Low Levels Using Cleavable 13C-Isotope-coded Affinity Tag and Multidimensional Chromatography
Mol. Cell. Proteomics, May 1, 2003; 2(5): 299 - 314.
[Abstract] [Full Text] [PDF]


Home page
Proc. Natl. Acad. Sci. USAHome page
D. P. Denning, S. S. Patel, V. Uversky, A. L. Fink, and M. Rexach
Disorder in the nuclear pore complex: The FG repeat regions of nucleoporins are natively unfolded
PNAS, March 4, 2003; 100(5): 2450 - 2455.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. ProteomicsHome page
N. P. C. Allen, S. S. Patel, L. Huang, R. J. Chalkley, A. Burlingame, M. Lutzmann, E. C. Hurt, and M. Rexach
Deciphering Networks of Protein Interactions at the Nuclear Pore Complex
Mol. Cell. Proteomics, December 1, 2002; 1(12): 930 - 946.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.