HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M200033-MCP200v1 1/7/509    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sanchez, J.-C. Articles by Cawthorne, M. A. Search for Related Content PubMed PubMed Citation Articles by Sanchez, J.-C. Articles by Cawthorne, M. A. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 1:509-516, 2002.
© 2002 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Effect of Rosiglitazone on the Differential Expression of Diabetes-associated Proteins in Pancreatic Islets of C57Bl/6 lep/lep Mice^*

Jean-Charles Sanchez^,, Véronique Converset, Anna Nolan^¶, Gerhard Schmid, Steven Wang^¶, Manfred Heller, Matthew V. Sennitt^¶, Denis F. Hochstrasser and Michael A. Cawthorne^¶

Geneva Proteomics Center, Central Clinical Chemistry Laboratory, Geneva University Hospital, 1211 Geneva 14, Switzerland
^¶ Clore Laboratory, School of Science, University of Buckingham, Buckingham MK18 1EG, United Kingdom

The insulin sensitizer drug, rosiglitazone, has been shown to have a protective effect on pancreatic islet cell structure and function in animal models of type 2 diabetes. The identification of new molecular targets associated both with islet cell dysfunction and protection is a crucial research goal. In the present study, a proteomics approach has been used to identify such targets. Obese C57Bl/6J lep/lep mice and lean littermates were given the insulin sensitizer drug BRL49653, rosiglitazone. It normalized the impaired glucose tolerance in lep/lep mice but had no significant effect on glucose tolerance in the lean mice. Pancreatic islet polypeptides were arrayed by a two-dimensional gel electrophoresis system that separated more than 2500 individual spots. Three overexpressed and six underexpressed proteins were significant (p < 0.05) between lep/lep and lean mice, and four were modulated significantly (p < 0.05) by the rosiglitazone treatment of the obese mice. The identity of these differentially expressed proteins was made using mass spectrometric analysis and provided evidence that differential expression of actin-binding proteins may be an important aspect of defective islet function. Rosiglitazone increased carboxypeptidase B expression in both lep/lep and normal mice suggesting that this might be an independent effect of rosiglitazone that contributes to improved insulin processing.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Suss, C. Czupalla, C. Winter, T. Pursche, K.-P. Knoch, M. Schroeder, B. Hoflack, and M. Solimena Rapid Changes of mRNA-binding Protein Levels following Glucose and 3-Isobutyl-1-methylxanthine Stimulation of Insulinoma INS-1 Cells Mol. Cell. Proteomics, March 1, 2009; 8(3): 393 - 408. [Abstract] [Full Text] [PDF]

				H. Lu, Y. Yang, E. M. Allister, N. Wijesekara, and M. B. Wheeler The Identification of Potential Factors Associated with the Development of Type 2 Diabetes: A Quantitative Proteomics Approach Mol. Cell. Proteomics, August 1, 2008; 7(8): 1434 - 1451. [Abstract] [Full Text] [PDF]

				G. M. Schmid, P. Meda, D. Caille, E. Wargent, J. O'Dowd, D. F. Hochstrasser, M. A. Cawthorne, and J.-C. Sanchez Inhibition of Insulin Secretion by Betagranin, an N-terminal Chromogranin A Fragment J. Biol. Chem., April 27, 2007; 282(17): 12717 - 12724. [Abstract] [Full Text] [PDF]

				Y. Wang, K. S. L. Lam, J. B. B. Lam, M. C. Lam, P. T. Y. Leung, M. Zhou, and A. Xu Overexpression of Angiopoietin-Like Protein 4 Alters Mitochondria Activities and Modulates Methionine Metabolic Cycle in the Liver Tissues of db/db Diabetic Mice Mol. Endocrinol., April 1, 2007; 21(4): 972 - 986. [Abstract] [Full Text] [PDF]

				E. M Scott, A. M Carter, and J. B. Findlay The application of proteomics to diabetes Diabetes and Vascular Disease Research, May 1, 2005; 2(2): 54 - 60. [Abstract] [PDF]

				E. Zeender, K. Maedler, D. Bosco, T. Berney, M. Y. Donath, and P. A. Halban Pioglitazone and Sodium Salicylate Protect Human {beta}-Cells against Apoptosis and Impaired Function Induced by Glucose and Interleukin-1{beta} J. Clin. Endocrinol. Metab., October 1, 2004; 89(10): 5059 - 5066. [Abstract] [Full Text] [PDF]

				M. Korc Diabetes Mellitus in the Era of Proteomics Mol. Cell. Proteomics, June 1, 2003; 2(6): 399 - 404. [Full Text] [PDF]