Advertisement
MCP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M200075-MCP200 on January 6, 2003.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M200075-MCP200v1
2/1/29    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Glossary
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hinsby, A. M.
Right arrow Articles by Mann, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hinsby, A. M.
Right arrow Articles by Mann, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 2:29-36, 2003.
© 2003 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Signaling Initiated by Overexpression of the Fibroblast Growth Factor Receptor-1 Investigated by Mass Spectrometry*

Anders M. Hinsby{ddagger},§,¶, Jesper V. Olsen{ddagger},, Keiryn L. Bennett{ddagger} and Matthias Mann{ddagger},||

{ddagger} MDS Proteomics A/S, Staermosegaardsvej 6, Odense DK-5230, Denmark, the
§ Protein Laboratory, Institute of Molecular Pathology, Blegdamsvej 3, Panum Institute 6.2, University of Copenhagen, Copenhagen DK-2200, Denmark, and the
|| Protein Interaction Laboratory, University of Southern Denmark, Campusvej 55, Odense DK-5230, Denmark

Overexpression of the fibroblast growth factor receptor-1 (FGFR-1), a prototypic receptor tyrosine kinase, is a feature of several human tumors. In human 293 cells overexpression of the FGFR-1 leads to constitutive activation of the receptor with concomitant sustained high increase in the cellular level of phosphotyrosine-containing proteins. Here we use mass spectrometry to study the tyrosine-phosphorylated proteins induced by overexpression of the FGFR-1. Several well known components of FGFR-1 signaling were identified along with two novel candidates: NS-1-associated protein-1 and target of Myb 1-like protein. We subsequently applied mass spectrometry precursor ion scanning to identify 22 tyrosine phosphorylation sites distributed on six substrate proteins of the FGFR-1 or downstream tyrosine kinases. Novel in vivo tyrosine phosphorylation sites were found in the FGFR-1, phospholipase C{gamma}, p90 ribosomal S6 kinase, cortactin, and NS-1-associated protein-1 as a result of sustained FGFR-1 signaling, and we propose these as functional links to downstream molecular and cellular processes.

To whom correspondence should be addressed. Tel.: 45-35-32-65-75; Fax: 45-35-36-01-16; E-mail: hinsby{at}plab.ku.dk


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
P. J. Boersema, L. Y. Foong, V. M. Y. Ding, S. Lemeer, B. van Breukelen, R. Philp, J. Boekhorst, B. Snel, J. den Hertog, A. B. H. Choo, et al.
In-depth Qualitative and Quantitative Profiling of Tyrosine Phosphorylation Using a Combination of Phosphopeptide Immunoaffinity Purification and Stable Isotope Dimethyl Labeling
Mol. Cell. Proteomics, January 1, 2010; 9(1): 84 - 99.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. R. St-Germain, P. Taylor, J. Tong, L. L. Jin, A. Nikolic, I. I. Stewart, R. M. Ewing, M. Dharsee, Z. Li, S. Trudel, et al.
Multiple myeloma phosphotyrosine proteomic profile associated with FGFR3 expression, ligand activation, and drug inhibition
PNAS, November 24, 2009; 106(47): 20127 - 20132.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Kang, S. Elf, S. Dong, T. Hitosugi, K. Lythgoe, A. Guo, H. Ruan, S. Lonial, H. J. Khoury, I. R. Williams, et al.
Fibroblast Growth Factor Receptor 3 Associates with and Tyrosine Phosphorylates p90 RSK2, Leading to RSK2 Activation That Mediates Hematopoietic Transformation
Mol. Cell. Biol., April 15, 2009; 29(8): 2105 - 2117.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Stuible, N. Dube, and M. L. Tremblay
PTP1B Regulates Cortactin Tyrosine Phosphorylation by Targeting Tyr446
J. Biol. Chem., June 6, 2008; 283(23): 15740 - 15746.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
B. S. Taylor, M. Pal, J. Yu, B. Laxman, S. Kalyana-Sundaram, R. Zhao, A. Menon, J. T. Wei, A. I. Nesvizhskii, D. Ghosh, et al.
Humoral Response Profiling Reveals Pathways to Prostate Cancer Progression
Mol. Cell. Proteomics, March 1, 2008; 7(3): 600 - 611.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
M. L. Miller, S. Hanke, A. M. Hinsby, C. Friis, S. Brunak, M. Mann, and N. Blom
Motif Decomposition of the Phosphotyrosine Proteome Reveals a New N-terminal Binding Motif for SHIP2
Mol. Cell. Proteomics, January 1, 2008; 7(1): 181 - 192.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
E. Com, C. Lagadec, A. Page, I. El Yazidi-Belkoura, C. Slomianny, A. Spencer, D. Hammache, B. B. Rudkin, and H. Hondermarck
Nerve Growth Factor Receptor TrkA Signaling in Breast Cancer Cells Involves Ku70 to Prevent Apoptosis
Mol. Cell. Proteomics, November 1, 2007; 6(11): 1842 - 1854.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
L.-Y. Tang, N. Deng, L.-S. Wang, J. Dai, Z.-L. Wang, X.-S. Jiang, S.-J. Li, L. Li, Q.-H. Sheng, D.-Q. Wu, et al.
Quantitative Phosphoproteome Profiling of Wnt3a-mediated Signaling Network: Indicating the Involvement of Ribonucleoside-diphosphate Reductase M2 Subunit Phosphorylation at Residue Serine 20 in Canonical Wnt Signal Transduction
Mol. Cell. Proteomics, November 1, 2007; 6(11): 1952 - 1967.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
Y. Zhang, A. Wolf-Yadlin, P. L. Ross, D. J. Pappin, J. Rush, D. A. Lauffenburger, and F. M. White
Time-resolved Mass Spectrometry of Tyrosine Phosphorylation Sites in the Epidermal Growth Factor Receptor Signaling Network Reveals Dynamic Modules
Mol. Cell. Proteomics, September 1, 2005; 4(9): 1240 - 1250.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L.-Y. C. Wing, H.-M. Chen, P.-C. Chuang, M.-H. Wu, and S.-J. Tsai
The Mammalian Target of Rapamycin-p70 Ribosomal S6 Kinase but Not Phosphatidylinositol 3-Kinase-Akt Signaling Is Responsible for Fibroblast Growth Factor-9-induced Cell Proliferation
J. Biol. Chem., May 20, 2005; 280(20): 19937 - 19947.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Bannai, K. Fukatsu, A. Mizutani, T. Natsume, S.-i. Iemura, T. Ikegami, T. Inoue, and K. Mikoshiba
An RNA-interacting Protein, SYNCRIP (Heterogeneous Nuclear Ribonuclear Protein Q1/NSAP1) Is a Component of mRNA Granule Transported with Inositol 1,4,5-Trisphosphate Receptor Type 1 mRNA in Neuronal Dendrites
J. Biol. Chem., December 17, 2004; 279(51): 53427 - 53434.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. M. Hinsby, J. V. Olsen, and M. Mann
Tyrosine Phosphoproteomics of Fibroblast Growth Factor Signaling: A ROLE FOR INSULIN RECEPTOR SUBSTRATE-4
J. Biol. Chem., November 5, 2004; 279(45): 46438 - 46447.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
H. Hondermarck
Breast Cancer: When Proteomics Challenges Biological Complexity
Mol. Cell. Proteomics, May 1, 2003; 2(5): 281 - 291.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
H. Steen, M. Fernandez, S. Ghaffari, A. Pandey, and M. Mann
Phosphotyrosine Mapping in Bcr/Abl Oncoprotein Using Phosphotyrosine-specific Immonium Ion Scanning
Mol. Cell. Proteomics, March 1, 2003; 2(3): 138 - 145.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement