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Molecular & Cellular Proteomics 2:37-49, 2003.
© 2003 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Protein Database, Human Retinal Pigment Epithelium^*

Karen A. West, Lin Yan, Karen Shadrach, Jian Sun, Azeem Hasan, Masaru Miyagi, John S. Crabb, Joe G. Hollyfield, Alan D. Marmorstein and John W. Crabb

From the Cole Eye Institute and Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195

The retinal pigment epithelium (RPE) is a single cell layer adjacent to the rod and cone photoreceptors that plays key roles in retinal physiology and the biochemistry of vision. RPE cells were isolated from normal adult human donor eyes, subcellular fractions were prepared, and proteins were fractionated by electrophoresis. Following in-gel proteolysis, proteins were identified by peptide sequencing using liquid chromatography tandem electrospray mass spectrometry and/or by peptide mass mapping using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Preliminary analyses have identified 278 proteins and provide a starting point for building a database of the human RPE proteome.

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