MCP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M300070-MCP200 on September 23, 2003.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M300070-MCP200v1
2/11/1198    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Li, J.
Right arrow Articles by Gygi, S. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Li, J.
Right arrow Articles by Gygi, S. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 2:1198-1204, 2003.
© 2003 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Protein Profiling with Cleavable Isotope-coded Affinity Tag (cICAT) Reagents

The Yeast Salinity Stress Response*,S

Jiaxu Li{ddagger}, Hanno Steen{ddagger} and Steven P. Gygi§

From the Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115-5730

Protein expression profiles in yeast cells, in response to salinity stress, were determined using the cleavable isotope-coded affinity tag (cICAT) labeling strategy. The analysis included separation of the mixed protein samples by SDS-PAGE, followed by excision of the entire gel lane, and division of the lane into 14 gel regions. Regions were subjected to in-gel digestion, biotin affinity chromatography, and analysis by nano-scale microcapillary liquid chromatography coupled to tandem mass spectrometry. The novel 13C-labeled ICAT reagents have identical elution profiles for labeled peptide pairs and broadly spread the distribution of labeled peptides during reversed-phase chromatography. A total of 560 proteins were identified and quantified, with 51 displaying more than 2-fold expression differences. In addition to some known proteins involved in salt stress, four RNA-binding proteins were found to be up-regulated by high salinity, suggesting that selective RNA export from the nucleus is important for the salt-stress response. Some proteins involved in amino acid synthesis, which have been observed to be up-regulated by amino acid starvation, were also found to increase their abundance on salt stress. These results indicate that salt stress and amino acid starvation cause overlapping cellular responses and are likely to be physiologically linked.

§ To whom correspondence should be addressed. Department of Cell Biology, 240 Longwood Avenue, Harvard Medical School, Boston, MA 02115-5730. E-mail: Steven_gygi{at}hms.harvard.edu.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
H. Rutschow, A. J. Ytterberg, G. Friso, R. Nilsson, and K. J. van Wijk
Quantitative Proteomics of a Chloroplast SRP54 Sorting Mutant and Its Genetic Interactions with CLPC1 in Arabidopsis
Plant Physiology, September 1, 2008; 148(1): 156 - 175.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. A. Galan, M. Guo, E. E. Sanchez, E. Cantu, A. Rodriguez-Acosta, J. C. Perez, and W. A. Tao
Quantitative Analysis of Snake Venoms Using Soluble Polymer-based Isotope Labeling
Mol. Cell. Proteomics, April 1, 2008; 7(4): 785 - 799.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
P. A. Everley, C. A. Gartner, W. Haas, A. Saghatelian, J. E. Elias, B. F. Cravatt, B. R. Zetter, and S. P. Gygi
Assessing Enzyme Activities Using Stable Isotope Labeling and Mass Spectrometry
Mol. Cell. Proteomics, October 1, 2007; 6(10): 1771 - 1777.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
C. P. Vaughn, D. K. Crockett, M. S. Lim, and K. S. J. Elenitoba-Johnson
Analytical Characteristics of Cleavable Isotope-Coded Affinity Tag-LC-Tandem Mass Spectrometry for Quantitative Proteomic Studies
J. Mol. Diagn., September 1, 2006; 8(4): 513 - 520.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
A. M. Redding, A. Mukhopadhyay, D. C. Joyner, T. C. Hazen, and J. D. Keasling
Study of nitrate stress in Desulfovibrio vulgaris Hildenborough using iTRAQ proteomics
Brief Funct Genomic Proteomic, June 1, 2006; 5(2): 133 - 143.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
D. Cheng, C. C. Hoogenraad, J. Rush, E. Ramm, M. A. Schlager, D. M. Duong, P. Xu, S. R. Wijayawardana, J. Hanfelt, T. Nakagawa, et al.
Relative and Absolute Quantification of Postsynaptic Density Proteome Isolated from Rat Forebrain and Cerebellum
Mol. Cell. Proteomics, June 1, 2006; 5(6): 1158 - 1170.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
T. Pisitkun, J. Bieniek, D. Tchapyjnikov, G. Wang, W. W. Wu, R.-F. Shen, and M. A. Knepper
High-throughput identification of IMCD proteins using LC-MS/MS
Physiol Genomics, April 13, 2006; 25(2): 263 - 276.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
M. B. Goshe
Characterizing phosphoproteins and phosphoproteomes using mass spectrometry
Brief Funct Genomic Proteomic, February 1, 2006; 4(4): 363 - 376.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
W. Majeran, Y. Cai, Q. Sun, and K. J. van Wijk
Functional Differentiation of Bundle Sheath and Mesophyll Maize Chloroplasts Determined by Comparative Proteomics
PLANT CELL, November 1, 2005; 17(11): 3111 - 3140.
[Abstract] [Full Text] [PDF]

Home page
 J Biomol ScreenHome page
R. J. Karreman and G. G. Lindsey
A Rapid Method to Determine the Stress Status of Saccharomyces cerevisiae by Monitoring the Expression of a Hsp12:Green Fluorescent Protein (GFP) Construct under the Control of the Hsp12 Promoter
J Biomol Screen, April 1, 2005; 10(3): 253 - 259.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
W.-C. Kao, Y.-R. Chen, E. C. Yi, H. Lee, Q. Tian, K.-M. Wu, S.-F. Tsai, S. S.-F. Yu, Y.-J. Chen, R. Aebersold, et al.
Quantitative Proteomic Analysis of Metabolic Regulation by Copper Ions in Methylococcus capsulatus (Bath)
J. Biol. Chem., December 3, 2004; 279(49): 51554 - 51560.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
C. Li, Y. Hong, Y.-X. Tan, H. Zhou, J.-H. Ai, S.-J. Li, L. Zhang, Q.-C. Xia, J.-R. Wu, H.-Y. Wang, et al.
Accurate Qualitative and Quantitative Proteomic Analysis of Clinical Hepatocellular Carcinoma Using Laser Capture Microdissection Coupled with Isotope-coded Affinity Tag and Two-dimensional Liquid Chromatography Mass Spectrometry
Mol. Cell. Proteomics, April 1, 2004; 3(4): 399 - 409.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.