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Originally published In Press as doi:10.1074/mcp.M300088-MCP200 on October 7, 2003.
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Molecular & Cellular Proteomics 2:1319-1330, 2003.
© 2003 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

A Proteomic Analysis of Arginine-methylated Protein Complexes*

François-Michel Boisvert{ddagger},§, Jocelyn Côté{ddagger},¶, Marie-Chloé Boulanger and Stéphane Richard||

From the Terry Fox Molecular Oncology Group and Bloomfield Center for Research on Aging, Lady Davis Institute for Medical Research and Departments of Oncology and Medicine, McGill University, Montréal, Québec H3T 1E2, Canada

Arginine methylation is a post-translational modification that results in the formation of asymmetrical and symmetrical dimethylated arginines (a- and sDMA). This modification is catalyzed by type I and II protein-arginine methyltransferases (PRMT), respectively. The two major enzymes PRMT1 (type I) and PRMT5 (type II) preferentially methylate arginines located in RG-rich clusters. Arginine methylation is a common modification, but the reagents for detecting this modification have been lacking. Thus, fewer than 20 proteins have been identified in the last 40 years as containing dimethylated arginines. We have generated previously four arginine methyl-specific antibodies; ASYM24 and ASYM25 are specific for aDMA, whereas SYM10 and SYM11 recognize sDMA. All of these antibodies were generated by using peptides with aDMA or sDMA in the context of different RG-rich sequences. HeLa cell extracts were used to purify the protein complexes recognized by each of the four antibodies, and the proteins were identified by microcapillary reverse-phase liquid chromatography coupled on line with electrospray ionization tandem mass spectrometry. The analysis of two tandem mass spectra for each methyl-specific antibody resulted in the identification of over 200 new proteins that are putatively arginine-methylated. The major protein complexes that were purified include components required for pre-mRNA splicing, polyadenylation, transcription, signal transduction, and cytoskeleton and DNA repair. These findings provide a basis for the identification of the role of arginine methylation in many cellular processes.

|| Recipient of a Canadian Institutes of Health Research Investigator Award. To whom correspondence should be addressed: Lady Davis Institute, 3755 Côte Ste.-Catherine Rd., Montréal, Québec H3T 1E2, Canada. Tel.: 514-340-8260; Fax: 514-340-8295; E-mail: stephane.richard{at}mcgill.ca


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