Advertisement
MCP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M300014-MCP200 on February 22, 2003.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M300014-MCP200v1
2/2/117    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Glossary
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carroll, J.
Right arrow Articles by Walker, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carroll, J.
Right arrow Articles by Walker, J. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 2:117-126, 2003.
© 2003 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Analysis of the Subunit Composition of Complex I from Bovine Heart Mitochondria*,S

Joe Carroll, Ian M. Fearnley, Richard J. Shannon, Judy Hirst and John E. Walker{ddagger}

From the Medical Research Council Dunn Human Nutrition Unit, The Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 2XY, United Kingdom

Complex I purified from bovine heart mitochondria is a multisubunit membrane-bound assembly. In the past, seven of its subunits were shown to be products of the mitochondrial genome, and 35 nuclear encoded subunits were identified. The complex is L-shaped with one arm in the plane of the membrane and the other lying orthogonal to it in the mitochondrial matrix. With mildly chaotropic detergents, the intact complex has been resolved into various subcomplexes. Subcomplex I{lambda} represents the extrinsic arm, subcomplex I{alpha} consists of subcomplex I{lambda} plus part of the membrane arm, and subcomplex Iß is another substantial part of the membrane arm. The intact complex and these three subcomplexes have been subjected to extensive reanalysis. Their subunits have been separated by three independent methods (one-dimensional SDS-PAGE, two-dimensional isoelectric focusing/SDS-PAGE, and reverse phase high pressure liquid chromatography (HPLC)) and analyzed by tryptic peptide mass fingerprinting and tandem mass spectrometry. The masses of many of the intact subunits have also been measured by electrospray ionization mass spectrometry and have provided valuable information about post-translational modifications. The presence of the known 35 nuclear encoded subunits in complex I has been confirmed, and four additional nuclear encoded subunits have been detected. Subunits B16.6, B14.7, and ESSS were discovered in the SDS-PAGE analysis of subcomplex I{lambda}, in the two-dimensional gel analysis of the intact complex, and in the HPLC analysis of subcomplex Iß, respectively. Despite many attempts, no sequence information has been obtained yet on a fourth new subunit (mass 10,566 ± 2 Da) also detected in the HPLC analysis of subcomplex Iß. It is unlikely that any more subunits of the bovine complex remain undiscovered. Therefore, the intact enzyme is a complex of 46 subunits, and, assuming there is one copy of each subunit in the complex, its mass is 980 kDa.

{ddagger} To whom correspondence should be addressed: Medical Research Council Dunn Human Nutrition Unit, The Wellcome Trust/MRC Bldg., Hills Rd., Cambridge CB2 2XY, UK. Tel.: 44-1223-252701; Fax: 44-1223-252705; E-mail: walker{at}mrc-dunn.cam.ac.uk


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. Torres-Bacete, P. K. Sinha, N. Castro-Guerrero, A. Matsuno-Yagi, and T. Yagi
Features of Subunit NuoM (ND4) in Escherichia coli NDH-1: TOPOLOGY AND IMPLICATION OF CONSERVED GLU144 FOR COUPLING SITE 1
J. Biol. Chem., November 27, 2009; 284(48): 33062 - 33069.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Feng, A. Witkowski, and S. Smith
Down-regulation of Mitochondrial Acyl Carrier Protein in Mammalian Cells Compromises Protein Lipoylation and Respiratory Complex I and Results in Cell Death
J. Biol. Chem., April 24, 2009; 284(17): 11436 - 11445.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. K. Hiltunen, M. S. Schonauer, K. J. Autio, T. M. Mittelmeier, A. J. Kastaniotis, and C. L. Dieckmann
Mitochondrial Fatty Acid Synthesis Type II: More than Just Fatty Acids
J. Biol. Chem., April 3, 2009; 284(14): 9011 - 9015.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Pineau, O. Layoune, A. Danon, and R. De Paepe
L-Galactono-1,4-lactone Dehydrogenase Is Required for the Accumulation of Plant Respiratory Complex I
J. Biol. Chem., November 21, 2008; 283(47): 32500 - 32505.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Nakamaru-Ogiso, A. Matsuno-Yagi, S. Yoshikawa, T. Yagi, and T. Ohnishi
Iron-Sulfur Cluster N5 Is Coordinated by an HXXXCXXCXXXXXC Motif in the NuoG Subunit of Escherichia coli NADH:Quinone Oxidoreductase (Complex I)
J. Biol. Chem., September 19, 2008; 283(38): 25979 - 25987.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. R. Hurd, R. Requejo, A. Filipovska, S. Brown, T. A. Prime, A. J. Robinson, I. M. Fearnley, and M. P. Murphy
Complex I within Oxidatively Stressed Bovine Heart Mitochondria Is Glutathionylated on Cys-531 and Cys-704 of the 75-kDa Subunit: POTENTIAL ROLE OF CYS RESIDUES IN DECREASING OXIDATIVE DAMAGE
J. Biol. Chem., September 5, 2008; 283(36): 24801 - 24815.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. Shimizu, L. Peng, F. Myouga, R. Motohashi, K. Shinozaki, and T. Shikanai
CRR23/NdhL is a Subunit of the Chloroplast NAD(P)H Dehydrogenase Complex in Arabidopsis
Plant Cell Physiol., May 1, 2008; 49(5): 835 - 842.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H. Lu and X. Cao
GRIM-19 Is Essential for Maintenance of Mitochondrial Membrane Potential
Mol. Biol. Cell, May 1, 2008; 19(5): 1893 - 1902.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. Ricci, V. Pastukh, J. Leonard, J. Turrens, G. Wilson, D. Schaffer, and S. W. Schaffer
Mitochondrial DNA damage triggers mitochondrial-superoxide generation and apoptosis
Am J Physiol Cell Physiol, February 1, 2008; 294(2): C413 - C422.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Lazarou, M. McKenzie, A. Ohtake, D. R. Thorburn, and M. T. Ryan
Analysis of the Assembly Profiles for Mitochondrial- and Nuclear-DNA-Encoded Subunits into Complex I
Mol. Cell. Biol., June 15, 2007; 27(12): 4228 - 4237.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. O. Vogel, C. E. J. Dieteren, L. P. W. J. van den Heuvel, P. H. G. M. Willems, J. A. M. Smeitink, W. J. H. Koopman, and L. G. J. Nijtmans
Identification of Mitochondrial Complex I Assembly Intermediates by Tracing Tagged NDUFS3 Demonstrates the Entry Point of Mitochondrial Subunits
J. Biol. Chem., March 9, 2007; 282(10): 7582 - 7590.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. L. Stephens, S. H. Lee, K. S. Paul, and P. T. Englund
Mitochondrial Fatty Acid Synthesis in Trypanosoma brucei
J. Biol. Chem., February 16, 2007; 282(7): 4427 - 4436.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
G. Pocsfalvi, M. Cuccurullo, G. Schlosser, S. Scacco, S. Papa, and A. Malorni
Phosphorylation of B14.5a Subunit from Bovine Heart Complex I Identified by Titanium Dioxide Selective Enrichment and Shotgun Proteomics
Mol. Cell. Proteomics, February 1, 2007; 6(2): 231 - 237.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. A. Wall, J. Wei, M. Ly, P. Belmont, J. J. Martindale, D. Tran, J. Sun, W. J. Chen, W. Yu, P. Oeller, et al.
Alterations in oxidative phosphorylation complex proteins in the hearts of transgenic mice that overexpress the p38 MAP kinase activator, MAP kinase kinase 6
Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2462 - H2472.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Carroll, I. M. Fearnley, and J. E. Walker
Definition of the mitochondrial proteome by measurement of molecular masses of membrane proteins
PNAS, October 31, 2006; 103(44): 16170 - 16175.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Carroll, I. M. Fearnley, J. M. Skehel, R. J. Shannon, J. Hirst, and J. E. Walker
Bovine Complex I Is a Complex of 45 Different Subunits
J. Biol. Chem., October 27, 2006; 281(43): 32724 - 32727.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
P. Cardol, M. Lapaille, P. Minet, F. Franck, R. F. Matagne, and C. Remacle
ND3 and ND4L Subunits of Mitochondrial Complex I, Both Nucleus Encoded in Chlamydomonas reinhardtii, Are Required for Activity and Assembly of the Enzyme.
Eukaryot. Cell, September 1, 2006; 5(9): 1460 - 1467.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
M. Kervinen, R. Hinttala, H. M. Helander, S. Kurki, J. Uusimaa, M. Finel, K. Majamaa, and I. E. Hassinen
The MELAS mutations 3946 and 3949 perturb the critical structure in a conserved loop of the ND1 subunit of mitochondrial complex I
Hum. Mol. Genet., September 1, 2006; 15(17): 2543 - 2552.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. B. Seo, E. Nakamaru-Ogiso, T. R. Flotte, A. Matsuno-Yagi, and T. Yagi
In Vivo Complementation of Complex I by the Yeast Ndi1 Enzyme: POSSIBLE APPLICATION FOR TREATMENT OF PARKINSON DISEASE
J. Biol. Chem., May 19, 2006; 281(20): 14250 - 14255.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Iuso, S. Scacco, C. Piccoli, F. Bellomo, V. Petruzzella, R. Trentadue, M. Minuto, M. Ripoli, N. Capitanio, M. Zeviani, et al.
Dysfunctions of Cellular Oxidative Metabolism in Patients with Mutations in the NDUFS1 and NDUFS4 Genes of Complex I
J. Biol. Chem., April 14, 2006; 281(15): 10374 - 10380.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Sunderhaus, N. V. Dudkina, L. Jansch, J. Klodmann, J. Heinemeyer, M. Perales, E. Zabaleta, E. J. Boekema, and H.-P. Braun
Carbonic Anhydrase Subunits Form a Matrix-exposed Domain Attached to the Membrane Arm of Mitochondrial Complex I in Plants
J. Biol. Chem., March 10, 2006; 281(10): 6482 - 6488.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
L. A. Sazanov and P. Hinchliffe
Structure of the Hydrophilic Domain of Respiratory Complex I from Thermus thermophilus
Science, March 10, 2006; 311(5766): 1430 - 1436.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
E. J. Anderson and P. D. Neufer
Type II skeletal myofibers possess unique properties that potentiate mitochondrial H2O2 generation
Am J Physiol Cell Physiol, March 1, 2006; 290(3): C844 - C851.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
E. McGregor and M. J. Dunn
Proteomics of the Heart: Unraveling Disease
Circ. Res., February 17, 2006; 98(3): 309 - 321.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. I. Grad, L. C. Sayles, and B. D. Lemire
Introduction of an additional pathway for lactate oxidation in the treatment of lactic acidosis and mitochondrial dysfunction in Caenorhabditis elegans
PNAS, December 20, 2005; 102(51): 18367 - 18372.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-R. Chen, C.-L. Chen, L. Zhang, K. B. Green-Church, and J. L. Zweier
Superoxide Generation from Mitochondrial NADH Dehydrogenase Induces Self-inactivation with Specific Protein Radical Formation
J. Biol. Chem., November 11, 2005; 280(45): 37339 - 37348.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. Carroll, I. M. Fearnley, J. M. Skehel, M. J. Runswick, R. J. Shannon, J. Hirst, and J. E. Walker
The Post-translational Modifications of the Nuclear Encoded Subunits of Complex I from Bovine Heart Mitochondria
Mol. Cell. Proteomics, May 1, 2005; 4(5): 693 - 699.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Nakamaru-Ogiso, T. Yano, T. Yagi, and T. Ohnishi
Characterization of the Iron-Sulfur Cluster N7 (N1c) in the Subunit NuoG of the Proton-translocating NADH-quinone Oxidoreductase from Escherichia coli
J. Biol. Chem., January 7, 2005; 280(1): 301 - 307.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
B. Schilling, S. Bharath M.M., R. H. Row, J. Murray, M. P. Cusack, R. A. Capaldi, C. R. Freed, K. N. Prasad, J. K. Andersen, and B. W. Gibson
Rapid Purification and Mass Spectrometric Characterization of Mitochondrial NADH Dehydrogenase (Complex I) from Rodent Brain and a Dopaminergic Neuronal Cell Line
Mol. Cell. Proteomics, January 1, 2005; 4(1): 84 - 96.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. Huang, H. Lu, A. Hao, D. C. H. Ng, S. Ponniah, K. Guo, C. Lufei, Q. Zeng, and X. Cao
GRIM-19, a Cell Death Regulatory Protein, Is Essential for Assembly and Function of Mitochondrial Complex I
Mol. Cell. Biol., October 1, 2004; 24(19): 8447 - 8456.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
C. Ugalde, R. Vogel, R. Huijbens, B. van den Heuvel, J. Smeitink, and L. Nijtmans
Human mitochondrial complex I assembles through the combination of evolutionary conserved modules: a framework to interpret complex I deficiencies
Hum. Mol. Genet., October 1, 2004; 13(20): 2461 - 2472.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
M. Zeviani and S. Di Donato
Mitochondrial disorders
Brain, October 1, 2004; 127(10): 2153 - 2172.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M.-C. Kao, S. Di Bernardo, M. Perego, E. Nakamaru-Ogiso, A. Matsuno-Yagi, and T. Yagi
Functional Roles of Four Conserved Charged Residues in the Membrane Domain Subunit NuoA of the Proton-translocating NADH-Quinone Oxidoreductase from Escherichia coli
J. Biol. Chem., July 30, 2004; 279(31): 32360 - 32366.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. Chen, I. M. Fearnley, S. Y. Peak-Chew, and J. E. Walker
The Phosphorylation of Subunits of Complex I from Bovine Heart Mitochondria
J. Biol. Chem., June 18, 2004; 279(25): 26036 - 26045.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Yadava, T. Houchens, P. Potluri, and I. E. Scheffler
Development and Characterization of a Conditional Mitochondrial Complex I Assembly System
J. Biol. Chem., March 26, 2004; 279(13): 12406 - 12413.
[Abstract] [Full Text] [PDF]

Home page
 Sci Aging Knowl EnvironHome page
B. W. Gibson
Exploiting Proteomics in the Discovery of Drugs That Target Mitochondrial Oxidative Damage
Sci. Aging Knowl. Environ., March 17, 2004; 2004(11): pe12 - pe12.
[Abstract] [Full Text]

Home page
 Hum Mol GenetHome page
C. Ugalde, R. J.R.J. Janssen, L. P. van den Heuvel, J. A.M. Smeitink, and L. G.J. Nijtmans
Differences in assembly or stability of complex I and other mitochondrial OXPHOS complexes in inherited complex I deficiency
Hum. Mol. Genet., March 15, 2004; 13(6): 659 - 667.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Stroh, O. Anderka, K. Pfeiffer, T. Yagi, M. Finel, B. Ludwig, and H. Schagger
Assembly of Respiratory Complexes I, III, and IV into NADH Oxidase Supercomplex Stabilizes Complex I in Paracoccus denitrificans
J. Biol. Chem., February 6, 2004; 279(6): 5000 - 5007.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Scacco, V. Petruzzella, S. Budde, R. Vergari, R. Tamborra, D. Panelli, L. P. van den Heuvel, J. A. Smeitink, and S. Papa
Pathological Mutations of the Human NDUFS4 Gene of the 18-kDa (AQDQ) Subunit of Complex I Affect the Expression of the Protein and the Assembly and Function of the Complex
J. Biol. Chem., November 7, 2003; 278(45): 44161 - 44167.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Antonicka, I. Ogilvie, T. Taivassalo, R. P. Anitori, R. G. Haller, J. Vissing, N. G. Kennaway, and E. A. Shoubridge
Identification and Characterization of a Common Set of Complex I Assembly Intermediates in Mitochondria from Patients with Complex I Deficiency
J. Biol. Chem., October 31, 2003; 278(44): 43081 - 43088.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Garofano, K. Zwicker, S. Kerscher, P. Okun, and U. Brandt
Two Aspartic Acid Residues in the PSST-Homologous NUKM Subunit of Complex I from Yarrowia lipolytica Are Essential for Catalytic Activity
J. Biol. Chem., October 24, 2003; 278(43): 42435 - 42440.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Murray, S. W. Taylor, B. Zhang, S. S. Ghosh, and R. A. Capaldi
Oxidative Damage to Mitochondrial Complex I Due to Peroxynitrite: IDENTIFICATION OF REACTIVE TYROSINES BY MASS SPECTROMETRY
J. Biol. Chem., September 26, 2003; 278(39): 37223 - 37230.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Gong, T. Xie, L. Yu, M. Hesterberg, D. Scheide, T. Friedrich, and C.-A. Yu
The Ubiquinone-binding Site in NADH:Ubiquinone Oxidoreductase from Escherichia coli
J. Biol. Chem., July 3, 2003; 278(28): 25731 - 25737.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement