MCP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M200051-MCP200 on January 17, 2003.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M200051-MCP200v1
2/2/70    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gromov, P.
Right arrow Articles by Celis, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gromov, P.
Right arrow Articles by Celis, J. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Molecular & Cellular Proteomics 2:70-84, 2003.
© 2003 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Protein Profiling of the Human Epidermis from the Elderly Reveals Up-regulation of a Signature of Interferon-{gamma}-induced Polypeptides That Includes Manganese-superoxide Dismutase and the p85ß Subunit of Phosphatidylinositol 3-Kinase*

Pavel Gromov{ddagger},§,¶, Gunhild Lange Skovgaard||, Hildur Palsdottir{ddagger},**, Irina Gromova{ddagger},§, Morten Østergaard{ddagger} and Julio E. Celis{ddagger},§,{ddagger}{ddagger}

{ddagger} Department of Medical Biochemistry and Danish Centre for Molecular Gerontology, The University of Aarhus, Ole Worms Allé, build. 170, DK-8000 Aarhus C, Denmark
§ Institute of Cancer Biology, The Danish Cancer Society, Strandboulevarden 49, DK-2100, Copenhagen, Denmark
|| Department of Dermatology, Bispebjerg Hospital, The University of Copenhagen, Bispebjerg Bakke 23, DK-2400 Copenhagen NV, Denmark and Laboratory of Molecular Gerontology and Dermatology, Danish Centre for Molecular Gerontology, The University of Aarhus, CF Mollers Allé, build. 130, DK-8000 Aarhus C, Denmark

Aging of the human skin is a complex process that consists of chronological and extrinsic aging, the latter caused mainly by exposure to ultraviolet radiation (photoaging). Here we present studies in which we have used proteomic profiling technologies and two-dimensional (2D) PAGE database resources to identify proteins whose expression is deregulated in the epidermis of the elderly. Fresh punch biopsies from the forearm of 20 pairs of young and old donors (21–30 and 75–92 years old, respectively) were dissected to yield an epidermal fraction that consisted mainly of differentiated cells. One- to two-mm3 epidermal pieces were labeled with [35S]methionine for 18 h, lysed, and subjected to 2D PAGE (isoelectric focusing and non-equilibrium pH gradient electrophoresis) and phosphorimage autoradiography. Proteins were identified by matching the gels with the master 2D gel image of human keratinocytes (proteomics.cancer.dk). In selected cases 2D PAGE immunoblotting and/or mass spectrometry confirmed the identity. Quantitative analysis of 172 well focused and abundant polypeptides showed that the level of most proteins (148) remains unaffected by the aging process. Twenty-two proteins were consistently deregulated by a factor of 1.5 or more across the 20 sample pairs. Among these we identified a group of six polypeptides (Mx-A, manganese-superoxide dismutase, tryptophanyl-tRNA synthetase, the p85ß subunit of phosphatidylinositol 3-kinase, and proteasomal proteins PA28-{alpha} and SSP 0107) that is induced by interferon-{gamma} in primary human keratinocytes and that represents a specific protein signature for the effect of this cytokine. Changes in the expression of the eukaryotic initiation factor 5A, NM23 H2, cyclophilin A, HSP60, annexin I, and plasminogen activator inhibitor 2 were also observed. Two proteins exhibited irregular behavior from individual to individual. Besides arguing for a role of interferon-{gamma} in the aging process, the biological activities associated with the deregulated proteins support the contention that aging is linked with increased oxidative stress that could lead to apoptosis in vivo.

To whom correspondence may be addressed: Inst. of Cancer Biology, The Danish Cancer Society, Strandboulevarden 49, DK-2100, Copenhagen. Fax: 45-35-25-77-21; E-mail: psg{at}cancer.dk

{ddagger}{ddagger} To whom correspondence may be addressed: Inst. of Cancer Biology, The Danish Cancer Society, Strandboulevarden 49, DK-2100, Copenhagen. Fax: 45-35-25-73-76; E-mail: jec{at}cancer.dk


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Kapoor, Q. Zhou, F. Otero, C. A. Myers, A. Bates, R. Belani, J. Liu, J.-K. Luo, E. Tzima, D.-E. Zhang, et al.
Evidence for Annexin II-S100A10 Complex and Plasmin in Mobilization of Cytokine Activity of Human TrpRS
J. Biol. Chem., January 25, 2008; 283(4): 2070 - 2077.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
Y. Miura, M. Kano, M. Yamada, T. Nishine, S. Urano, S. Suzuki, T. Endo, and T. Toda
Proteomic Study on X-irradiation-responsive Proteins and Ageing: Search for Responsible Proteins for Radiation Adaptive Response
J. Biochem., August 1, 2007; 142(2): 145 - 155.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. Liu, E. Shue, K. L. Ewalt, and P. Schimmel
A new {gamma}-interferon-inducible promoter and splice variants of an anti-angiogenic human tRNA synthetase
Nucleic Acids Res., February 2, 2004; 32(2): 719 - 727.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.