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Molecular & Cellular Proteomics 2:85-95, 2003.
© 2003 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Proteome Analysis of Intact Proteins in Complex Mixtures^*

Julia R. Hayter, Duncan H. L. Robertson, Simon J. Gaskell and Robert J. Beynon^,¶

Department of Veterinary Preclinical Sciences, University of Liverpool, Crown Street Liverpool L69 7ZJ, United Kingdom
Michael Barber Centre for Mass Spectrometry, Department of Chemistry, University of Manchester Institute of Science and Technology, P.O. Box 88, Manchester M60 1QD, United Kingdom

Analysis of intact protein mixtures by electrospray ionization mass spectrometry requires the resolution of a complex, overlapping set of multiply charged envelopes. To ascertain the ability of a moderate resolution mass spectrometer to resolve such mixtures, we have analyzed the soluble proteins of adult chick skeletal muscle. This is a highly specialized tissue showing a marked bias in expression of glycolytic enzymes in the soluble fraction. SDS-PAGE-resolved proteins were first identified by a combination of matrix-assisted laser desorption ionization time-of-flight (TOF) and electrospray ionization tandem mass spectrometry. Then the mixture of intact proteins was introduced into the electrospray source of a Q-TOF mass spectrometer either by direct infusion or via a C4 desalting trap. In both instances, the complex pattern of peaks could be resolved into true masses, and these masses could in many instances be reconciled with the masses predicted from the known protein sequences when qualified by expected co- and post-translational modifications. These included loss of the N-terminal initiator methionine residue and N-terminal acetylation. The ability to resolve such a complex mixture of proteins with a routine instrument is of considerable value in analyses of protein expression and in the confirmation of post-translational changes in mature proteins.

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