Advertisement
MCP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M300026-MCP200 on May 16, 2003.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M300026-MCP200v1
2/4/262    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Glossary
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Steel, L. F.
Right arrow Articles by Block, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Steel, L. F.
Right arrow Articles by Block, T.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 2:262-270, 2003.
© 2003 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Efficient and Specific Removal of Albumin from Human Serum Samples*

Laura F. Steel{ddagger},§, Michael G. Trotter{ddagger}, Pamela B. Nakajima, Taj S. Mattu||, Gregory Gonye** and Timothy Block{ddagger}

{ddagger} Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Jefferson Center for Biomedical Research, Doylestown, Pennsylvania 18901
Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111
|| Isis Innovation, Ewert House, Ewert Place, Summertown, Oxford OX2 7SG, United Kingdom
** Daniel Baugh Institute for Functional Genomics/Computational Biology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

Patient serum or plasma is frequently monitored for biochemical markers of disease or physiological status. Many of the rapidly evolving technologies of proteome analysis are being used to find additional clinically informative protein markers. The unusually high abundance of albumin in serum can interfere with the resolution and sensitivity of many proteome profiling techniques. We have used monoclonal antibodies against human serum albumin (HSA) to develop an immunoaffinity resin that is effective in the removal of both full-length HSA and many of the HSA fragments present in serum. This resin shows markedly better performance than dye-based resins in terms of both the efficiency and specificity of albumin removal. Immunoglobulins are another class of highly abundant serum protein. When protein G resin is used together with our immunoaffinity resin, Ig proteins and HSA can be removed in a single step. This strategy could be extended to the removal of any protein for which specific antibodies or affinity reagents are available.

§ To whom correspondence should be addressed: Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Jefferson Center for Biomedical Research, Doylestown, PA 18901. Tel.: 215-489-4946; Fax: 215-489-4920; E-mail: laura.steel{at}jefferson.edu


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. ProteomicsHome page
T. Fortin, A. Salvador, J. P. Charrier, C. Lenz, X. Lacoux, A. Morla, G. Choquet-Kastylevsky, and J. Lemoine
Clinical Quantitation of Prostate-specific Antigen Biomarker in the Low Nanogram/Milliliter Range by Conventional Bore Liquid Chromatography-Tandem Mass Spectrometry (Multiple Reaction Monitoring) Coupling and Correlation with ELISA Tests
Mol. Cell. Proteomics, May 1, 2009; 8(5): 1006 - 1015.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
N. Seam, D. A. Gonzales, S. J. Kern, G. L. Hortin, G. T. Hoehn, and A. F. Suffredini
Quality Control of Serum Albumin Depletion for Proteomic Analysis
Clin. Chem., November 1, 2007; 53(11): 1915 - 1920.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
J. Harezlak, M. Wang, D. Christiani, and X. Lin
Quantitative quality-assessment techniques to compare fractionation and depletion methods in SELDI-TOF mass spectrometry experiments
Bioinformatics, September 15, 2007; 23(18): 2441 - 2448.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
F. W. Khwaja, J. S. Duke-Cohan, D. J. Brat, and E. G. Van Meir
Attractin Is Elevated in the Cerebrospinal Fluid of Patients with Malignant Astrocytoma and Mediates Glioma Cell Migration.
Clin. Cancer Res., November 1, 2006; 12(21): 6331 - 6336.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J.-B. Vincourt, F. Lionneton, G. Kratassiouk, F. Guillemin, P. Netter, D. Mainard, and J. Magdalou
Establishment of a Reliable Method for Direct Proteome Characterization of Human Articular Cartilage
Mol. Cell. Proteomics, October 1, 2006; 5(10): 1984 - 1995.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2003 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement