MCP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


Originally published In Press as doi:10.1074/mcp.M300094-MCP200 on October 30, 2003.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M300094-MCP200v1
3/1/73    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ding, S.-J.
Right arrow Articles by Xia, Q.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ding, S.-J.
Right arrow Articles by Xia, Q.-C.
Social Bookmarking
 Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 3:73-81, 2004.
© 2004 by The American Society for Biochemistry and Molecular Biology, Inc.


Research

From Proteomic Analysis to Clinical Significance

Overexpression of Cytokeratin 19 Correlates with Hepatocellular Carcinoma Metastasis *

Shi-Jian Ding{ddagger},§, Yan Li§, Ye-Xiong Tan§,||, Man-Rong Jiang{ddagger}, Bo Tian, Ying-Kun Liu, Xiao-Xia Shao{ddagger}, Sheng-Long Ye, Jia-Rui Wu{ddagger}, Rong Zeng{ddagger}, Hong-Yang Wang||,**, Zhao-You Tang,** and Qi-Chang Xia{ddagger},**

From the {ddagger} Research Center for Proteome Analysis, Key Laboratory of Proteomics, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, CAS, Shanghai 200031, People’s Republic of China; Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, People’s Republic of China; and || Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, People’s Republic of China

To better understand the mechanism underlying the hepatocellular carcinoma (HCC) metastasis and to search potential markers for HCC prognosis, differential proteomic analysis on two well-established HCC cell strains with high and low metastatic potentials, MHCC97-H and MHCC97-L, was conducted using two-dimensional gel electrophoresis followed by matrix-assisted laser desorption/time-of-flight mass spectrometry. Cytokeratin 19 (CK19) was identified and found to be overexpressed in MHCC97-H as compared with MHCC97-L. This result was further confirmed by two-dimensional Western blot analysis and immunofluorescence assay. Furthermore, one-dimensional Western blot analysis showed consistently increased CK19 expression in progressively more metastatic cells. Immunohistochemical study on 102 human HCC specimens revealed that more patients in the CK19-positive group had overt intrahepatic metastases (satellite nodules, p < 0.05; vascular tumor emboli, p < 0.001; tumor node metastatis staging, p < 0.001). CK19 fragment CYFRA 21-1 levels measured in sera from nude mice model of human HCC metastasis with radioimmunoassay increased in parallel with tumor progression and rose remarkably when pulmonary metastases occurred. The results demonstrated that overexpression of CK19 in HCC cells is related to metastatic behavior. Serum CK19 level might reflect the pathological progression in some HCC and may be a useful marker for predicting tumor metastasis and a therapeutic target for the treatment of HCC patients with metastases.


§ S.-J. D., Y. L., and Y.-X. T. contributed equally to this paper

** To whom correspondence should be addressed: Qi-Chang Xia, Shanghai Institute of Biochemistry and Cell Biology, Yueyang Road, Shanghai 200031, China. Tel.: 86-21-54920160; Fax: 86-21-54920171; E-mail: xiaqc{at}sibs.ac.cn; Hong-Yang Wang, Eastern Hepatobiliary Surgery Hospital, No. 225, Changhai Road, Shanghai 200438, China. Tel.: 86-21-25070856; Fax: 86-21-65566851; E-mail: hywangk{at}online.sh.cn; or Zhao-You Tang, Liver Cancer Institute & Zhongshan Hospital of Fudan University, 136 Yixueyuan Road, Shanghai 200032, China. Tel.: 86-21-64037181; Fax: 86-21-64037181; E-mail: zytang{at}scrap.stc.sh.cn


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
Clin. Cancer Res.Home page
X.-R. Yang, Y. Xu, G.-M. Shi, J. Fan, J. Zhou, Y. Ji, H.-C. Sun, S.-J. Qiu, B. Yu, Q. Gao, et al.
Cytokeratin 10 and Cytokeratin 19: Predictive Markers for Poor Prognosis in Hepatocellular Carcinoma Patients after Curative Resection
Clin. Cancer Res., June 15, 2008; 14(12): 3850 - 3859.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
T. Yamashita, M. Forgues, W. Wang, J. W. Kim, Q. Ye, H. Jia, A. Budhu, K. A. Zanetti, Y. Chen, L.-X. Qin, et al.
EpCAM and {alpha}-Fetoprotein Expression Defines Novel Prognostic Subtypes of Hepatocellular Carcinoma
Cancer Res., March 1, 2008; 68(5): 1451 - 1461.
[Abstract] [Full Text] [PDF]


Home page
Clin. Cancer Res.Home page
J.-Z. Pang, L.-X. Qin, N. Ren, Z.-Y. Hei, Q.-H. Ye, W.-D. Jia, B.-S. Sun, G.-L. Lin, D.-Y. Liu, Y.-K. Liu, et al.
Loss of Heterozygosity at D8S298 Is a Predictor for Long-term Survival of Patients with Tumor-Node-Metastasis Stage I of Hepatocellular Carcinoma
Clin. Cancer Res., December 15, 2007; 13(24): 7363 - 7369.
[Abstract] [Full Text] [PDF]


Home page
Cancer Res.Home page
T. Yamashita, A. Budhu, M. Forgues, and X. W. Wang
Activation of Hepatic Stem Cell Marker EpCAM by Wnt {beta}-Catenin Signaling in Hepatocellular Carcinoma
Cancer Res., November 15, 2007; 67(22): 10831 - 10839.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. ProteomicsHome page
W. Sun, B. Xing, Y. Sun, X. Du, M. Lu, C. Hao, Z. Lu, W. Mi, S. Wu, H. Wei, et al.
Proteome Analysis of Hepatocellular Carcinoma by Two-dimensional Difference Gel Electrophoresis: Novel Protein Markers in Hepatocellular Carcinoma Tissues
Mol. Cell. Proteomics, October 1, 2007; 6(10): 1798 - 1808.
[Abstract] [Full Text] [PDF]


Home page
INT J SURG PATHOLHome page
K. O. Alsaad, S. Serra, A. Perren, E. Hsieh, and R. Chetty
CK19 and CD99 Immunoexpression Profile in Goblet Cell (Mucin-Producing Neuroendocrine Tumors) and Classical Carcinoids of the Vermiform Appendix
International Journal of Surgical Pathology, July 1, 2007; 15(3): 252 - 257.
[Abstract] [PDF]


Home page
Mol. Cell. ProteomicsHome page
C. Li, Y. Hong, Y.-X. Tan, H. Zhou, J.-H. Ai, S.-J. Li, L. Zhang, Q.-C. Xia, J.-R. Wu, H.-Y. Wang, et al.
Accurate Qualitative and Quantitative Proteomic Analysis of Clinical Hepatocellular Carcinoma Using Laser Capture Microdissection Coupled with Isotope-coded Affinity Tag and Two-dimensional Liquid Chromatography Mass Spectrometry
Mol. Cell. Proteomics, April 1, 2004; 3(4): 399 - 409.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.