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Molecular & Cellular Proteomics 3:1065-1082, 2004.
© 2004 by The American Society for Biochemistry and Molecular Biology, Inc.


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,¶,**,

From the Departments of
Biomedical Engineering,
Pediatrics, and ¶ Pharmacology, and || Charles B. Stout Neuroscience Mass Spectrometry Laboratory, University of Tennessee Health Science Center and ** Department of Veterans Affairs Medical Center, Memphis, TN 38104
When cultured in low serum-containing growth medium, the mouse C2C12 cells exit cell cycle and undergo a well-defined program of differentiation that culminates in the formation of myosin heavy chain-positive bona fide multinucleated muscle cells. To gain an understanding into this process, we compared total, membrane- and nuclear-enriched proteins, and phospho-proteins from the proliferating C2C12 cells and the fully differentiated myotubes by the combined methods of two-dimensional PAGE, quantitative PDQuest image analysis, and MS. Quantification of more than 2,000 proteins from C2C12 myoblasts and myotubes revealed that a vast majority of the abundant proteins appear to be relegated to the essential, housekeeping and structural functions, and their steady state levels remain relatively constant. In contrast, 75 proteins were highly regulated during the phenotypic conversion of rapidly dividing C2C12 myoblasts into fully differentiated, multi-nucleated, post-mitotic myotubes. We found that differential accumulation of 26 phospho-proteins also occurred during conversion of C2C12 myoblasts into myotubes. We identified the differentially expressed proteins by MALDI-TOF-MS and LC-ESI-quadrupole ion trap MS/MS. We demonstrate that more than 100 proteins, some shown to be associated with muscle differentiation for the first time, that regulate inter- and intracellular signaling, cell shape, proliferation, apoptosis, and gene expression impinge on the mechanism of skeletal muscle differentiation.

To whom correspondence should be addressed: University of Tennessee Health Science Center & Department of Veterans Affairs Medical Center, 1030 Jefferson Avenue, Memphis, TN 38104. Tel.: 901-523-8990 (ext. 7634); Fax: 901-577-7273; E-mail: rraghow{at}utmem.edu
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