HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M400106-MCP200v1 3/11/1119    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bonilha, V. L. Articles by Hollyfield, J. G. Search for Related Content PubMed PubMed Citation Articles by Bonilha, V. L. Articles by Hollyfield, J. G. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 3:1119-1127, 2004.
© 2004 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Proteomic Characterization of Isolated Retinal Pigment Epithelium Microvilli^*,S

Vera L. Bonilha, Sanjoy K. Bhattacharya, Karen A. West, Jian Sun, John W. Crabb, Mary E. Rayborn and Joe G. Hollyfield

From The Cole Eye Institute, Department of Ophthalmic Research, The Cleveland Clinic Foundation, Cleveland, OH 44195

Polarized epithelial cells are characterized by displaying compartmentalized functions associated with differential distribution of transporters, structural proteins, and signaling molecules on their apical and basolateral surfaces. Their apical surfaces frequently elaborate microvilli, which vary in structure according to the specific type and function of each epithelium. The molecular basis of this heterogeneity is poorly understood. However, differences in function will undoubtedly be reflected in the specific molecular composition of the apical surface in each epithelial subtype. We have exploited a method for isolating microvilli from the mouse eye using wheat germ agglutinin (WGA)-agarose beads to begin to understand the specific molecular composition of apical microvilli of the retinal pigment epithelium (RPE) and expand our knowledge of the potential function of this interface. Initially, apical RPE plasma membranes bound to WGA beads were processed for morphological analysis using known apical and basolateral surface markers. The protein composition of the apical microvilli was then established using proteomic analysis. Over 200 proteins were identified, including a number of proteins previously known to be localized to RPE microvilli, as well as others not known to be present at this surface. Localization of novel proteins identified with proteomics was confirmed by immunohistochemistry in both mouse and rat eye tissue. The data generated provides new information on the protein composition of the RPE apical microvilli. The isolation technique used should be amenable for isolating microvilli in other epithelia as well, allowing new insights into additional functions of this important epithelial compartment.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				K. M. Farjo, G. Moiseyev, Y. Takahashi, R. K. Crouch, and J.-x. Ma The 11-cis-Retinol Dehydrogenase Activity of RDH10 and Its Interaction with Visual Cycle Proteins Invest. Ophthalmol. Vis. Sci., November 1, 2009; 50(11): 5089 - 5097. [Abstract] [Full Text] [PDF]

				A.-L. Law, Q. Ling, K. A. Hajjar, C. E. Futter, J. Greenwood, P. Adamson, S. T. Wavre-Shapton, S. E. Moss, and M. J. Hayes Annexin A2 Regulates Phagocytosis of Photoreceptor Outer Segments in the Mouse Retina Mol. Biol. Cell, September 1, 2009; 20(17): 3896 - 3904. [Abstract] [Full Text] [PDF]

				Y. F. Leung, P. Ma, and J. E. Dowling Gene Expression Profiling of Zebrafish Embryonic Retinal Pigment Epithelium In Vivo Invest. Ophthalmol. Vis. Sci., February 1, 2007; 48(2): 881 - 890. [Abstract] [Full Text] [PDF]

				M. Liden and U. Eriksson Understanding Retinol Metabolism: Structure and Function of Retinol Dehydrogenases J. Biol. Chem., May 12, 2006; 281(19): 13001 - 13004. [Full Text] [PDF]

				K. C. S. Rao, V. Palamalai, J. R. Dunlevy, and M. Miyagi Peptidyl-Lys Metalloendopeptidase-catalyzed 18O Labeling for Comparative Proteomics: Application to Cytokine/Lipolysaccharide-treated Human Retinal Pigment Epithelium Cell Line Mol. Cell. Proteomics, October 1, 2005; 4(10): 1550 - 1557. [Abstract] [Full Text] [PDF]