HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M300104-MCP200v1 3/3/250    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by McLean, L. Articles by Beynon, R. J. Search for Related Content PubMed PubMed Citation Articles by McLean, L. Articles by Beynon, R. J. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 3:250-256, 2004.
© 2004 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

A Proteome Analysis of the Subcutaneous Gel in Avian Hatchlings^*

Lynn McLean, Mary K. Doherty, D. Charles Deeming and Robert J. Beynon^,¶

From the Department of Veterinary Preclinical Sciences, University of Liverpool, Liverpool L69 7ZJ, United Kingdom; and Hatchery Consulting and Research, Lincoln LN2 3LP, United Kingdom

An appropriate level of water loss from eggs is critical to successful hatching. This water may be lost from the egg by evaporation, but where water loss is suboptimal, it is commonly observed that the hatchlings contain substantial amounts of a subcutaneous gel-like fluid. To characterize this fluid, we have analyzed the proteins that are contained within it. The protein complement comprised a small number of proteins in high concentrations. Proteomics analysis of the constituent proteins identified virtually all of these abundant proteins and confirmed that the subcutaneous gel was very similar in protein composition to plasma. However, the subcutaneous gel was substantially depleted of fibrinogen. It is possible that activation of the final stages of the coagulation process might account for the enhanced viscosity, creating a gel-like material that is relatively immobile in the subcutaneous space. This gel may function as a water volume that is partitioned during embryonic development in order to mitigate the effects of high water content of the egg caused by low mass loss during incubation and in some instances might also function as a water reserve to support the hatchling in the first few hours of life free of the shell.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				L. A. Cogburn, T. E. Porter, M. J. Duclos, J. Simon, S. C. Burgess, J. J. Zhu, H. H. Cheng, J. B. Dodgson, and J. Burnside Functional Genomics of the Chicken A Model Organism Poult. Sci., October 1, 2007; 86(10): 2059 - 2094. [Abstract] [Full Text] [PDF]

				J. Rivers, D. M. Simpson, D. H. L. Robertson, S. J. Gaskell, and R. J. Beynon Absolute Multiplexed Quantitative Analysis of Protein Expression during Muscle Development Using QconCAT Mol. Cell. Proteomics, August 1, 2007; 6(8): 1416 - 1427. [Abstract] [Full Text] [PDF]