HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M300076-MCP200v1 3/6/558    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Bulau, P. Articles by Peter-Katalinic, J. Search for Related Content PubMed PubMed Citation Articles by Bulau, P. Articles by Peter-Katalinic, J. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 3:558-564, 2004.
© 2004 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Identification of Neuropeptides from the Sinus Gland of the Crayfish Orconectes limosus Using Nanoscale On-line Liquid Chromatography Tandem Mass Spectrometry^*

Patrick Bulau^,, Iris Meisen, Tina Schmitz, Rainer Keller and Jasna Peter-Katalini^,¶

From the Institute for Zoophysiology, University of Bonn, Endenicher Allee 11-13, D-53115 Bonn, Germany; and Institute for Medical Physics and Biophysics, Biomedical Analytics, University of Münster, Robert-Koch-Straße 31, D-48149 Münster, Germany

In this article, a novel and sensitive analytical strategy for direct characterization of neuropeptides from the X-Organ-sinus gland neurosecretory system of the crayfish Orconectes limosus is presented. A desalted extract corresponding to 0.5 sinus gland equivalents was analyzed in a nanoflow liquid chromatography system coupled to quadrupole time-of-flight tandem mass spectrometry (nanoLC-QTOF MS/MS). The existence and structural identity of four crustacean hyperglycemic hormone precursor-related peptide variants and two new genetic variants of the pigment-dispersing hormone, not detected by conventional chromatographic systems, molecular cloning, or immunochemical methods before, was revealed. The here-presented approach of the combined LC-QTOF MS/MS technique is a powerful tool to discover new peptide hormones in biological systems, due to its sensitivity, accuracy, and speed.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?