Advertisement
MCP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M400001-MCP200 on April 1, 2004.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M400001-MCP200v1
3/7/675    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Glossary
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marmagne, A.
Right arrow Articles by Ephritikhine, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marmagne, A.
Right arrow Articles by Ephritikhine, G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 3:675-691, 2004.
© 2004 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Identification of New Intrinsic Proteins in Arabidopsis Plasma Membrane Proteome*,S

Anne Marmagne{ddagger}, Marie-Aude Rouet{ddagger}, Myriam Ferro§, Norbert Rolland, Carine Alcon{ddagger},||, Jacques Joyard, Jérome Garin§, Hélène Barbier-Brygoo{ddagger} and Geneviève Ephritikhine{ddagger},**

From the {ddagger} Institut des Sciences du Végétal, Centre National de la Recherche Scientifique–Unité Propre de Recherche (CNRS–UPR 2355), 91198 Gif sur Yvette Cedex, France; § Laboratoire de Chimie des Protéines, ERM-0201 (Institut National de la Santé et de la Recherche Médicale (INSERM)/Commissariat à l’Energie Atomique (CEA)) Département Réponse et Dynamique Cellulaires, and Laboratoire de Physiologie Cellulaire Végétale, Unité Mixte de Recherche (UMR) 5168 (CEA/CNRS/INRA/Université Joseph Fourier), F-38054 Grenoble-Cedex 9, France

Identification and characterization of anion channel genes in plants represent a goal for a better understanding of their central role in cell signaling, osmoregulation, nutrition, and metabolism. Though channel activities have been well characterized in plasma membrane by electrophysiology, the corresponding molecular entities are little documented. Indeed, the hydrophobic protein equipment of plant plasma membrane still remains largely unknown, though several proteomic approaches have been reported. To identify new putative transport systems, we developed a new proteomic strategy based on mass spectrometry analyses of a plasma membrane fraction enriched in hydrophobic proteins. We produced from Arabidopsis cell suspensions a highly purified plasma membrane fraction and characterized it in detail by immunological and enzymatic tests. Using complementary methods for the extraction of hydrophobic proteins and mass spectrometry analyses on mono-dimensional gels, about 100 proteins have been identified, 95% of which had never been found in previous proteomic studies. The inventory of the plasma membrane proteome generated by this approach contains numerous plasma membrane integral proteins, one-third displaying at least four transmembrane segments. The plasma membrane localization was confirmed for several proteins, therefore validating such proteomic strategy. An in silico analysis shows a correlation between the putative functions of the identified proteins and the expected roles for plasma membrane in transport, signaling, cellular traffic, and metabolism. This analysis also reveals 10 proteins that display structural properties compatible with transport functions and will constitute interesting targets for further functional studies.

** To whom correspondence should be addressed: Institut des Sciences du Végétal, Batiment 22, CNRS–UPR 2355, F-91198 Gif sur Yvette Cedex, France. Tel.: 33-(0)1-69-82-37-93; Fax: 33-(0)1-69-82-37 68; E-mail: Genevieve.Ephritikhine{at}isv.cnrs-gif.fr


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
A. Honsbein, S. Sokolovski, C. Grefen, P. Campanoni, R. Pratelli, M. Paneque, Z. Chen, I. Johansson, and M. R. Blatt
A Tripartite SNARE-K+ Channel Complex Mediates in Channel-Dependent K+ Nutrition in Arabidopsis
PLANT CELL, September 1, 2009; 21(9): 2859 - 2877.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. Myouga, C. Hosoda, T. Umezawa, H. Iizumi, T. Kuromori, R. Motohashi, Y. Shono, N. Nagata, M. Ikeuchi, and K. Shinozaki
A Heterocomplex of Iron Superoxide Dismutases Defends Chloroplast Nucleoids against Oxidative Stress and Is Essential for Chloroplast Development in Arabidopsis
PLANT CELL, November 1, 2008; 20(11): 3148 - 3162.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
W. Tang, Z. Deng, J. A. Oses-Prieto, N. Suzuki, S. Zhu, X. Zhang, A. L. Burlingame, and Z.-Y. Wang
Proteomics Studies of Brassinosteroid Signal Transduction Using Prefractionation and Two-dimensional DIGE
Mol. Cell. Proteomics, April 1, 2008; 7(4): 728 - 738.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
J. Stout, E. Romero-Severson, M. O. Ruegger, and C. Chapple
Semidominant Mutations in Reduced Epidermal Fluorescence 4 Reduce Phenylpropanoid Content in Arabidopsis
Genetics, April 1, 2008; 178(4): 2237 - 2251.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
A. Marmagne, M. Ferro, T. Meinnel, C. Bruley, L. Kuhn, J. Garin, H. Barbier-Brygoo, and G. Ephritikhine
A High Content in Lipid-modified Peripheral Proteins and Integral Receptor Kinases Features in the Arabidopsis Plasma Membrane Proteome
Mol. Cell. Proteomics, November 1, 2007; 6(11): 1980 - 1996.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Raffaele, S. Mongrand, P. Gamas, A. Niebel, and T. Ott
Genome-Wide Annotation of Remorins, a Plant-Specific Protein Family: Evolutionary and Functional Perspectives
Plant Physiology, November 1, 2007; 145(3): 593 - 600.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
U. G. Schmidt, A. Endler, S. Schelbert, A. Brunner, M. Schnell, H. E. Neuhaus, D. Marty-Mazars, F. Marty, S. Baginsky, and E. Martinoia
Novel Tonoplast Transporters Identified Using a Proteomic Approach with Vacuoles Isolated from Cauliflower Buds
Plant Physiology, September 1, 2007; 145(1): 216 - 229.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. J. Benschop, S. Mohammed, M. O'Flaherty, A. J. R. Heck, M. Slijper, and F. L. H. Menke
Quantitative Phosphoproteomics of Early Elicitor Signaling in Arabidopsis
Mol. Cell. Proteomics, July 1, 2007; 6(7): 1198 - 1214.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Marinova, L. Pourcel, B. Weder, M. Schwarz, D. Barron, J.-M. Routaboul, I. Debeaujon, and M. Klein
The Arabidopsis MATE Transporter TT12 Acts as a Vacuolar Flavonoid/H+-Antiporter Active in Proanthocyanidin-Accumulating Cells of the Seed Coat
PLANT CELL, June 1, 2007; 19(6): 2023 - 2038.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Sanderfoot
Increases in the Number of SNARE Genes Parallels the Rise of Multicellularity among the Green Plants
Plant Physiology, May 1, 2007; 144(1): 6 - 17.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
B. Lefebvre, F. Furt, M.-A. Hartmann, L. V. Michaelson, J.-P. Carde, F. Sargueil-Boiron, M. Rossignol, J. A. Napier, J. Cullimore, J.-J. Bessoule, et al.
Characterization of Lipid Rafts from Medicago truncatula Root Plasma Membranes: A Proteomic Study Reveals the Presence of a Raft-Associated Redox System
Plant Physiology, May 1, 2007; 144(1): 402 - 418.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. W. Murcha, D. Elhafez, R. Lister, J. Tonti-Filippini, M. Baumgartner, K. Philippar, C. Carrie, D. Mokranjac, J. Soll, and J. Whelan
Characterization of the Preprotein and Amino Acid Transporter Gene Family in Arabidopsis
Plant Physiology, January 1, 2007; 143(1): 199 - 212.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
C. J. Nelson, A. D. Hegeman, A. C. Harms, and M. R. Sussman
A Quantitative Analysis of Arabidopsis Plasma Membrane Using Trypsin-catalyzed 18O Labeling
Mol. Cell. Proteomics, August 1, 2006; 5(8): 1382 - 1395.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. Morel, S. Claverol, S. Mongrand, F. Furt, J. Fromentin, J.-J. Bessoule, J.-P. Blein, and F. Simon-Plas
Proteomics of Plant Detergent-resistant Membranes
Mol. Cell. Proteomics, August 1, 2006; 5(8): 1396 - 1411.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Schmidt, G. Gessner, M. Luff, I. Heiland, V. Wagner, M. Kaminski, S. Geimer, N. Eitzinger, T. Reissenweber, O. Voytsekh, et al.
Proteomic Analysis of the Eyespot of Chlamydomonas reinhardtii Provides Novel Insights into Its Components and Tactic Movements
PLANT CELL, August 1, 2006; 18(8): 1908 - 1930.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Zalejski, S. Paradis, R. Maldiney, Y. Habricot, E. Miginiac, J.-P. Rona, and E. Jeannette
Induction of Abscisic Acid-Regulated Gene Expression by Diacylglycerol Pyrophosphate Involves Ca2+ and Anion Currents in Arabidopsis Suspension Cells
Plant Physiology, August 1, 2006; 141(4): 1555 - 1562.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. J. Ytterberg, J.-B. Peltier, and K. J. van Wijk
Protein Profiling of Plastoglobules in Chloroplasts and Chromoplasts. A Surprising Site for Differential Accumulation of Metabolic Enzymes
Plant Physiology, March 1, 2006; 140(3): 984 - 997.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Seigneurin-Berny, A. Gravot, P. Auroy, C. Mazard, A. Kraut, G. Finazzi, D. Grunwald, F. Rappaport, A. Vavasseur, J. Joyard, et al.
HMA1, a New Cu-ATPase of the Chloro plast Envelope, Is Essential for Growth under Adverse Light Conditions
J. Biol. Chem., February 3, 2006; 281(5): 2882 - 2892.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J.-B. Peltier, Y. Cai, Q. Sun, V. Zabrouskov, L. Giacomelli, A. Rudella, A. J. Ytterberg, H. Rutschow, and K. J. van Wijk
The Oligomeric Stromal Proteome of Arabidopsis thaliana Chloroplasts
Mol. Cell. Proteomics, January 1, 2006; 5(1): 114 - 133.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J.-B. Peltier, A. J. Ytterberg, Q. Sun, and K. J. van Wijk
New Functions of the Thylakoid Membrane Proteome of Arabidopsis thaliana Revealed by a Simple, Fast, and Versatile Fractionation Strategy
J. Biol. Chem., November 19, 2004; 279(47): 49367 - 49383.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E. Alexandersson, G. Saalbach, C. Larsson, and P. Kjellbom
Arabidopsis Plasma Membrane Proteomics Identifies Components of Transport, Signal Transduction and Membrane Trafficking
Plant Cell Physiol., November 15, 2004; 45(11): 1543 - 1556.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
B. Favery, L. A. Chelysheva, M. Lebris, F. Jammes, A. Marmagne, J. de Almeida-Engler, P. Lecomte, C. Vaury, R. A. Arkowitz, and P. Abad
Arabidopsis Formin AtFH6 Is a Plasma Membrane-Associated Protein Upregulated in Giant Cells Induced by Parasitic Nematodes
PLANT CELL, September 1, 2004; 16(9): 2529 - 2540.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2004 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement