|
|
||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Molecular & Cellular Proteomics 3:788-795, 2004.
© 2004 by The American Society for Biochemistry and Molecular Biology, Inc.
,


,¶,||,**
From the
Department of Pathology & Laboratory Medicine, Brown University Medical School, Providence, RI 02912; ¶ Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University Medical School, Providence, RI 02912; || Department of Surgery, Division of Surgical Research, Rhode Island Hospital and Brown University Medical School, Providence, RI 02912
Previously, we demonstrated that antibodies printed on a solid support were able to detect protein-protein interaction in mammalian cells. Here we further developed the antibody array system for detecting proteins with various post-translational modifications in mammalian cells. In this novel approach, immunoprecipitated proteins were labeled with fluorescent dye followed by incubation over antibody arrays. Targeted proteins, captured by the antibodies immobilized on PVDF membrane or glass slide, were detected by means of near infrared fluorescent scanner or fluorescent microscopy. To demonstrate the application of the antibody arrays in protein post-translational modifications, we profiled protein tyrosine phosphorylation, ubiquitination, and acetylation in mammalian cells under different conditions. Our results indicate that antibody array technology can provide a powerful means of profiling a large number of proteins with different post-translational modifications in cells.
![]()
CiteULike
Complore
Connotea
Del.icio.us
Digg
Reddit
Technorati What's this?
This article has been cited by other articles:
![]() |
J. Hu, X. He, K. A. Baggerly, K. R. Coombes, B. T.J. Hennessy, and G. B. Mills Non-parametric quantification of protein lysate arrays Bioinformatics, August 1, 2007; 23(15): 1986 - 1994. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. E. Krueger and S. Srivastava Posttranslational Protein Modifications: Current Implications for Cancer Detection, Prevention, and Therapeutics Mol. Cell. Proteomics, October 1, 2006; 5(10): 1799 - 1810. [Full Text] [PDF] |
||||
![]() |
M. Sanchez-Carbayo Antibody Arrays: Technical Considerations and Clinical Applications in Cancer Clin. Chem., September 1, 2006; 52(9): 1651 - 1659. [Abstract] [Full Text] [PDF] |
||||
![]() |
A. S. Chung, Y.-j. Guan, Z.-L. Yuan, J. E. Albina, and Y. E. Chin Ankyrin Repeat and SOCS Box 3 (ASB3) Mediates Ubiquitination and Degradation of Tumor Necrosis Factor Receptor II Mol. Cell. Biol., June 1, 2005; 25(11): 4716 - 4726. [Abstract] [Full Text] [PDF] |
||||
![]() |
B. B. Haab Antibody Arrays in Cancer Research Mol. Cell. Proteomics, April 1, 2005; 4(4): 377 - 383. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Journal of Lipid Research | ASBMB Today |