HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: R400009-MCP200v1 3/9/851    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Coulombe, B. Articles by Bergeron, D. Search for Related Content PubMed PubMed Citation Articles by Coulombe, B. Articles by Bergeron, D. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 3:851-856, 2004.
© 2004 by The American Society for Biochemistry and Molecular Biology, Inc.

---

PERSPECTIVE

Interaction Networks of the Molecular Machines That Decode, Replicate, and Maintain the Integrity of the Human Genome ^*

Benoit Coulombe^,, Célia Jeronimo, Marie-France Langelier, Marilena Cojocaru and Dominique Bergeron

From the Laboratory of Gene Transcription, Institut de Recherches Cliniques de Montréal, 110 Avenue des Pins Ouest, Montréal, Québec, Canada H2W 1R7

The interaction of many proteins with genomic DNA is required for the expression, replication, and maintenance of the integrity of mammalian genomes. These proteins participate in processes as diverse as gene transcription and mRNA processing, as well as in DNA replication, recombination, and repair. This intricate system, where the various nuclear machineries interact with one another and bind to either common or distinct DNA regions to create an impressive network of protein-protein and protein-DNA interactions, is made even more complex by the need for a very stringent control in order to ensure normal cell growth and differentiation. A general methodology based on the in vivo pull-down of tagged components of nuclear machines and regulatory proteins was used to study genome-wide protein-protein and protein-DNA interactions in mammalian cells. In particular, this approach has been used in defining the interaction networks (or "interactome") formed by RNA polymerase II, a molecular machine that decodes the human genome. In addition, because this methodology allows for the purification of variant forms of tagged complexes having site-directed mutations in key elements, it can also be used for deciphering the relationship between the structure and the function of the molecular machines, such as RNA polymerase II, that by binding DNA play a central role in the pathway from the genome to the organism.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				O. V. Singh and N. S. Nagaraj Transcriptomics, proteomics and interactomics: unique approaches to track the insights of bioremediation Briefings in Functional Genomics, February 1, 2006; 4(4): 355 - 362. [Abstract] [Full Text] [PDF]