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Originally published In Press as doi:10.1074/mcp.M400045-MCP200 on May 28, 2004.
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Molecular & Cellular Proteomics 3:857-871, 2004.
© 2004 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Identification and Verification of Novel Rodent Postsynaptic Density Proteins*,S

Bryen A. Jordan{ddagger}, Brian D. Fernholz§, Muriel Boussac, Chongfeng Xu, Gabriela Grigorean, Edward B. Ziff{ddagger},§ and Thomas A. Neubert,||

From the {ddagger} Department of Biochemistry, § Department of Physiology and Neuroscience, and Department of Pharmacology and Skirball Institute of Biomolecular Medicine, New York University School of Medicine, New York, NY 10016

The postsynaptic density (PSD) is a cellular structure specialized in receiving and transducing synaptic information. Here we describe the identification of 452 proteins isolated from biochemically purified PSD fractions of rat and mouse brains using nanoflow HPLC coupled to electrospray tandem mass spectrometry (LC-MS/MS). Fluorescence microscopy and Western blotting were used to verify that many of the novel proteins identified exhibit subcellular distributions consistent with those of PSD-localized proteins. In addition to identifying most previously described PSD components, we also detected proteins involved in signaling to the nucleus as well as regulators of ADP-ribosylation factor signaling, ubiquitination, RNA trafficking, and protein translation. These results suggest new mechanisms by which the PSD helps regulate synaptic strength and transmission.

|| To whom correspondence should be addressed: Skirball Institute of Biomolecular Medicine Lab 5-18, New York University School of Medicine, New York, NY 10016. Tel.: 212-263-7265; Fax: 212-263-8214; E-mail: neubert{at}saturn.med.nyu.edu


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