HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M400087-MCP200v1 4/1/1    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kolkman, A. Articles by Slijper, M. Search for Related Content PubMed PubMed Citation Articles by Kolkman, A. Articles by Slijper, M. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 4:1-11, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Comparative Proteome Analysis of Saccharomyces cerevisiae Grown in Chemostat Cultures Limited for Glucose or Ethanol^*

Annemieke Kolkman, Maurien M. A. Olsthoorn, Carola E. M. Heeremans, Albert J. R. Heck and Monique Slijper^,¶

From the Department of Biomolecular Mass Spectrometry, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CA Utrecht, The Netherlands; and DSM Food Specialties, R&D, Department of Analysis, 2600 MA Delft, The Netherlands

The use of chemostat culturing enables investigation of steady-state physiological characteristics and adaptations to nutrient-limited growth, while all other relevant growth conditions are kept constant. We examined and compared the proteomic response of wild-type Saccharomyces cerevisiae CEN.PK113-7D to growth in aerobic chemostat cultures limited for carbon sources being either glucose or ethanol. To obtain a global overview of changes in the proteome, we performed triplicate analyses using two-dimensional gel electrophoresis and identified proteins of interest using MS. Relative quantities of about 400 proteins were obtained and analyzed statistically to determine which protein steady-state expression levels changed significantly under glucose- or ethanol-limited conditions. Interestingly, only enzymes involved in central carbon metabolism showed a significant change in steady-state expression, whereas expression was only detected in one of both carbon source-limiting conditions for 15 of these enzymes. Side effects that were previously reported for batch cultivation conditions, such as responses to continuous variation of specific growth rate, to carbon-catabolite repression, and to accumulation of toxic substrates, were not observed. Moreover, by comparing our proteome data with corresponding mRNA data, we were able to unravel which processes in the central carbon metabolism were regulated at the level of the proteome, and which processes at the level of transcriptome. Importantly, we show here that the combined approach of chemostat cultivation and comprehensive proteome analysis allowed us to study the primary effect of single limiting conditions on the yeast proteome.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				F.-M. Lin, B. Qiao, and Y.-J. Yuan Comparative Proteomic Analysis of Tolerance and Adaptation of Ethanologenic Saccharomyces cerevisiae to Furfural, a Lignocellulosic Inhibitory Compound Appl. Envir. Microbiol., June 1, 2009; 75(11): 3765 - 3776. [Abstract] [Full Text] [PDF]

				M. J. L. de Groot, P. Daran-Lapujade, B. van Breukelen, T. A. Knijnenburg, E. A. F. de Hulster, M. J. T. Reinders, J. T. Pronk, A. J. R. Heck, and M. Slijper Quantitative proteomics and transcriptomics of anaerobic and aerobic yeast cultures reveals post-transcriptional regulation of key cellular processes Microbiology, November 1, 2007; 153(11): 3864 - 3878. [Abstract] [Full Text] [PDF]

				D. J. V. Beste, E. Laing, B. Bonde, C. Avignone-Rossa, M. E. Bushell, and J. J. McFadden Transcriptomic Analysis Identifies Growth Rate Modulation as a Component of the Adaptation of Mycobacteria to Survival inside the Macrophage J. Bacteriol., June 1, 2007; 189(11): 3969 - 3976. [Abstract] [Full Text] [PDF]