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Molecular & Cellular Proteomics 4:1718-1724, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Proteomics-based Identification of Human Acute Leukemia Antigens That Induce Humoral Immune Response^*

Jiu-wei Cui^,,¶, Wei-hua Li^,¶, Jie Wang, Ai-ling Li, Hui-yan Li, Hong-xia Wang, Kun He, Wei Li, Li-hua Kang, Ming Yu, Bei-fen Shen, Guan-Jun Wang^,|| and Xue-min Zhang^,||,**

From the Institute of Basic Medical Sciences, National Center of Biomedical Analysis, Beijing 100850, China and the Department of Hematology and Oncology, the First Clinical Hospital of Jilin University, Changchun 130021, China

The identification of panels of tumor antigens that elicit an antibody response may have utility in cancer screening, diagnosis, and establishing prognosis. Until now, autoimmunity in cancer has been mainly revealed in solid tumors. The aim of this study was to apply the proteomic approach to the identification of proteins that commonly elicit a humoral response in acute leukemia (AL). Sera from 21 newly diagnosed patients with AL, 20 patients with solid tumors, and 22 noncancer controls were analyzed for antibody-based reactivity against AL proteins resolved by two-dimensional electrophoresis. As a result, autoantibody against a protein identified by mass spectrometry as Rho GDP dissociation inhibitor 2 was detected in sera from 15 of 21 patients with AL (71%). By contrast, such antibody was detected in sera from one of 20 patients with solid tumors (5%) and one of 22 noncancer controls (4.5%). Five other protein autoantibodies were also found in AL patients with a high frequency and constituted the major target antigens of the AL autoimmune response. The findings of autoantibodies against Rho GDP dissociation inhibitor 2 and other proteins in sera of patients with AL suggest that the proteomic approach we have implemented may have utility for the development of a serum-based assay for AL screening and diagnosis.

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