MCP Sign the guestbook
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M500102-MCP200 on August 9, 2005.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M500102-MCP200v1
4/11/1741    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hood, B. L.
Right arrow Articles by Krizman, D. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hood, B. L.
Right arrow Articles by Krizman, D. B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 4:1741-1753, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Proteomic Analysis of Formalin-fixed Prostate Cancer Tissue*,S

Brian L. Hood{ddagger}, Marlene M. Darfler§, Thomas G. Guiel§, Bungo Furusato, David A. Lucas{ddagger}, Bradley R. Ringeisen||, Isabell A. Sesterhenn, Thomas P. Conrads{ddagger}, Timothy D. Veenstra{ddagger},** and David B. Krizman{ddagger},{ddagger}{ddagger}

From the {ddagger} Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc., National Cancer Institute, Frederick, MD 21702; § Expression Pathology Incorporated, Gaithersburg, MD 20877; Armed Forces Institute of Pathology, Department of Genitourinary Pathology, Washington, DC 20306; and || Naval Research Laboratory, Washington, DC 20375

Proteomic analysis of formalin-fixed paraffin-embedded (FFPE) tissue would enable retrospective biomarker investigations of this vast archive of pathologically characterized clinical samples that exist worldwide. These FFPE tissues are, however, refractory to proteomic investigations utilizing many state of the art methodologies largely due to the high level of covalently cross-linked proteins arising from formalin fixation. A novel tissue microdissection technique has been developed and combined with a method to extract soluble peptides directly from FFPE tissue for mass spectral analysis of prostate cancer (PCa) and benign prostate hyperplasia (BPH). Hundreds of proteins from PCa and BPH tissue were identified, including several known PCa markers such as prostate-specific antigen, prostatic acid phosphatase, and macrophage inhibitory cytokine-1. Quantitative proteomic profiling utilizing stable isotope labeling confirmed similar expression levels of prostate-specific antigen and prostatic acid phosphatase in BPH and PCa cells, whereas the expression of macrophage inhibitory cytokine-1 was found to be greater in PCa as compared with BPH cells.

** To whom correspondence may be addressed: Laboratory of Proteomics and Analytical Technologies, National Cancer Institute, SAIC-Frederick, Inc., P.O. Box B, Frederick, MD 21702. Tel.: 301-846-7286; Fax: 301-846-6037; E-mail: veenstra{at}ncifcrf.gov

{ddagger}{ddagger} To whom correspondence may be addressed: Expression Pathology Inc., 9290 Gaither Rd., Gaithersburg, MD 20877. Tel.: 301-977-3654; Fax: 301-926-9283; E-mail: d.krizman{at}expressionpathology.com


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Mol. Diagn.Home page
C. Xu, J. R. Houck, W. Fan, P. Wang, Y. Chen, M. Upton, N. D. Futran, S. M. Schwartz, L. P. Zhao, C. Chen, et al.
Simultaneous Isolation of DNA and RNA from the Same Cell Population Obtained by Laser Capture Microdissection for Genome and Transcriptome Profiling
J. Mol. Diagn., March 1, 2008; 10(2): 129 - 134.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
V. Patel, B. L. Hood, A. A. Molinolo, N. H. Lee, T. P. Conrads, J. C. Braisted, D. B. Krizman, T. D. Veenstra, and J. S. Gutkind
Proteomic Analysis of Laser-Captured Paraffin-Embedded Tissues: A Molecular Portrait of Head and Neck Cancer Progression
Clin. Cancer Res., February 15, 2008; 14(4): 1002 - 1014.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
T. Guo, W. Wang, P. A. Rudnick, T. Song, J. Li, Z. Zhuang, R. J. Weil, D. L. DeVoe, C. S. Lee, and B. M. Balgley
Proteome Analysis of Microdissected Formalin-fixed and Paraffin-embedded Tissue Specimens
J. Histochem. Cytochem., July 1, 2007; 55(7): 763 - 772.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
M. S. Lim and K. S. J. Elenitoba-Johnson
Mass Spectrometry-based Proteomic Studies of Human Anaplastic Large Cell Lymphoma
Mol. Cell. Proteomics, October 1, 2006; 5(10): 1787 - 1798.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
B. L. Hood, T. P. Conrads, and T. D. Veenstra
Unravelling the proteome of formalin-fixed paraffin-embedded tissue
Brief Funct Genomic Proteomic, June 1, 2006; 5(2): 169 - 175.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.