MCP AbD Serotec
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M500129-MCP200 on August 23, 2005.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M500129-MCP200v1
M500129-MCP200v2
4/11/1797    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kofler, M.
Right arrow Articles by Freund, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kofler, M.
Right arrow Articles by Freund, C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 4:1797-1811, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

GYF Domain Proteomics Reveals Interaction Sites in Known and Novel Target Proteins*,S

Michael Kofler, Kathrin Motzny and Christian Freund{ddagger}

From the Protein Engineering Group, Forschungsinstitut für Molekulare Pharmakologie and Freie Universität Berlin, Robert-Rössle-Str. 10, 13125 Berlin, Germany

GYF domains are conserved eukaryotic adaptor domains that recognize proline-rich sequences. Although the structure and function of the prototypic GYF domain from the human CD2BP2 protein have been characterized in detail, very little is known about GYF domains from other proteins and species. Here we describe the binding properties of four GYF domains of various origins. Phage display in combination with SPOT analysis revealed the PPG(F/I/L/M/V) motif as a general recognition signature. Based on these results, the proteomes of human, yeast, and Arabidopsis thaliana were searched for potential interaction sites. Binding of several candidate proteins was confirmed by pull-down experiments or yeast two-hybrid analysis. The binding epitope of the GYF domain from the yeast SMY2 protein was mapped by NMR spectroscopy and led to a structural model that accounts for the different binding properties of SMY2-type GYF domains and the CD2BP2-GYF domain.

{ddagger} To whom correspondence should be addressed. Tel.: 49-030- 94793-181; Fax: 49-030-94793-189; E-mail: freund{at}fmp-berlin.de


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Int ImmunolHome page
M. Heinze, M. Kofler, and C. Freund
Investigating the functional role of CD2BP2 in T cells
Int. Immunol., November 1, 2007; 19(11): 1313 - 1318.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Balaji and L. Aravind
The RAGNYA fold: a novel fold with multiple topological variants found in functionally diverse nucleic acid, nucleotide and peptide-binding proteins
Nucleic Acids Res., September 27, 2007; 35(17): 5658 - 5671.
[Abstract] [Full Text] [PDF]

Home page
 Protein Eng Des SelHome page
A. Georgiev, M. Sjostrom, and A. Wieslander
Binding specificities of the GYF domains from two Saccharomyces cerevisiae Paralogs
Protein Eng. Des. Sel., September 4, 2007; (2007) gzm041v1.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
E. Song, S. Gao, R. Tian, S. Ma, H. Huang, J. Guo, Y. Li, L. Zhang, and Y. Gao
A High Efficiency Strategy for Binding Property Characterization of Peptide-binding Domains
Mol. Cell. Proteomics, August 1, 2006; 5(8): 1368 - 1381.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.