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Molecular & Cellular Proteomics 4:1826-1830, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

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Dataset

A Catalogue of Human Saliva Proteins Identified by Free Flow Electrophoresis-based Peptide Separation and Tandem Mass Spectrometry^*,S

Hongwei Xie, Nelson L. Rhodus, Robert J. Griffin^¶, John V. Carlis^|| and Timothy J. Griffin^,**

From the Departments of Biochemistry, Molecular Biology, and Biophysics, Oral Medicine, Diagnosis, and Radiology, School of Dentistry, ^¶ Therapeutic Radiology-Radiation Oncology, and ^|| Computer Science and Engineering, University of Minnesota, Minneapolis, Minnesota 55455

Human saliva has great potential for clinical disease diagnostics. Constructing a comprehensive catalogue of saliva proteins using proteomic approaches is a necessary first step to identifying potential protein biomarkers of disease. However, because of the challenge presented in cataloguing saliva proteins with widely varying abundance, new proteomic approaches are needed. To this end, we used a newly developed approach coupling peptide separation using free flow electrophoresis with linear ion trap tandem mass spectrometry to identify proteins in whole human saliva. We identified 437 proteins with high confidence (false positive rate below 1%), producing the largest catalogue of proteins from a single saliva sample to date and providing new information on the composition and potential diagnostic utility of this fluid. The statistically validated, transparently presented, and annotated dataset provides a model for presenting large scale proteomic data of this type, which should facilitate better dissemination and easier comparisons of proteomic datasets from future studies in saliva.

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