HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M500213-MCP200v1 4/12/1942    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Janzi, M. Articles by Nilsson, P. Search for Related Content PubMed PubMed Citation Articles by Janzi, M. Articles by Nilsson, P. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 4:1942-1947, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Serum Microarrays for Large Scale Screening of Protein Levels ^*,S

Magdalena Janzi^,, Jenny Ödling^,¶, Qiang Pan-Hammarström, Mårtenn Sundberg^¶, Joakim Lundeberg^¶, Mathias Uhlén^¶, Lennart Hammarström^,|| and Peter Nilsson^¶,**

From the Division of Clinical Immunology, Karolinska Institute, Karolinska Hospital in Huddinge, SE-14186 Stockholm, Sweden and ^¶ Department of Molecular Biotechnology, KTH-Royal Institute of Technology, AlbaNova University Center, SE-10691, Stockholm, Sweden

There is a great need for comprehensive proteomic analysis of large patient cohorts of plasma and serum samples to identify biomarkers of human diseases. Here we describe a new antibody-based proteomic approach involving a reverse array format where serum samples are spotted on a microarray. This enables all samples to be screened for their content of a certain serum protein in a single experiment using target-recognizing antibodies and fluorescently labeled secondary antibodies. The procedure is illustrated with the analysis of the IgA levels in 2009 spotted serum samples, and the data are compared with clinical routine measurements. The results suggest that it is possible to simultaneously screen thousands of complex clinical serum samples for their content of the relative amount of specific serum proteins of clinical relevance.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				W.-J. Qian, J. M. Jacobs, T. Liu, D. G. Camp II, and R. D. Smith Advances and Challenges in Liquid Chromatography-Mass Spectrometry-based Proteomics Profiling for Clinical Applications Mol. Cell. Proteomics, October 1, 2006; 5(10): 1727 - 1744. [Abstract] [Full Text] [PDF]

				M. Sanchez-Carbayo Antibody Arrays: Technical Considerations and Clinical Applications in Cancer Clin. Chem., September 1, 2006; 52(9): 1651 - 1659. [Abstract] [Full Text] [PDF]