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Originally published In Press as doi:10.1074/mcp.M500213-MCP200 on August 29, 2005.
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Molecular & Cellular Proteomics 4:1942-1947, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Serum Microarrays for Large Scale Screening of Protein Levels *,S

Magdalena Janzi{ddagger},§, Jenny Ödling§,¶, Qiang Pan-Hammarström{ddagger}, Mårtenn Sundberg, Joakim Lundeberg, Mathias Uhlén, Lennart Hammarström{ddagger},|| and Peter Nilsson,**

From the {ddagger} Division of Clinical Immunology, Karolinska Institute, Karolinska Hospital in Huddinge, SE-14186 Stockholm, Sweden and Department of Molecular Biotechnology, KTH-Royal Institute of Technology, AlbaNova University Center, SE-10691, Stockholm, Sweden

There is a great need for comprehensive proteomic analysis of large patient cohorts of plasma and serum samples to identify biomarkers of human diseases. Here we describe a new antibody-based proteomic approach involving a reverse array format where serum samples are spotted on a microarray. This enables all samples to be screened for their content of a certain serum protein in a single experiment using target-recognizing antibodies and fluorescently labeled secondary antibodies. The procedure is illustrated with the analysis of the IgA levels in 2009 spotted serum samples, and the data are compared with clinical routine measurements. The results suggest that it is possible to simultaneously screen thousands of complex clinical serum samples for their content of the relative amount of specific serum proteins of clinical relevance.

|| To whom correspondence may be addressed. Tel.: 46-8-5248-3586; Fax: 46-8-5248-3588; E-mail: lennart.hammarstrom{at}labmed.ki.se


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