HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M400128-MCP200v1 4/2/133    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bagshaw, R. D. Articles by Callahan, J. W. Search for Related Content PubMed PubMed Citation Articles by Bagshaw, R. D. Articles by Callahan, J. W. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 4:133-143, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

A Proteomic Analysis of Lysosomal Integral Membrane Proteins Reveals the Diverse Composition of the Organelle^*,S

Richard D. Bagshaw^,, Don J. Mahuran^, and John W. Callahan^,¶,||,**

From the Research Institute, The Hospital for Sick Children, Toronto M5G 1X8, Canada and the Departments of Laboratory Medicine and Pathobiology, ^¶ Biochemistry, and ^|| Pediatrics, University of Toronto, Toronto, Ontario M5S 1A1, Canada

Lysosomes are endocytic subcellular compartments that contribute to the degradation and recycling of cellular material. Using highly purified rat liver tritosomes (Triton WR1339-filled lysosomes) and an ion exchange chromatography/LC-tandem MS-based protein/peptide separation and identification procedure, we characterized the major integral membrane protein complement of this organelle. While many of the 215 proteins we identified have been previously associated with lysosomes and endosomes, others have been associated with the endoplasmic reticulum, Golgi, cytosol, plasma membrane, and lipid rafts. At least 20 proteins were identified as unknown cDNAs that have no orthologues of known function, and 35 proteins were identified that function in protein and vesicle trafficking. This latter group includes multiple Rab and SNARE proteins as well as ubiquitin. Defining the roles of these proteins in the lysosomal membrane will assist in elucidating novel lysosomal functions involved in cellular homeostasis and pathways that are affected in various disease processes.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Hoves, J. A. Trapani, and I. Voskoboinik The battlefield of perforin/granzyme cell death pathways J. Leukoc. Biol., February 1, 2010; 87(2): 237 - 243. [Abstract] [Full Text] [PDF]

				S. L. Hall, S. Hester, J. L. Griffin, K. S. Lilley, and A. P. Jackson The Organelle Proteome of the DT40 Lymphocyte Cell Line Mol. Cell. Proteomics, June 1, 2009; 8(6): 1295 - 1305. [Abstract] [Full Text] [PDF]

				W. Shui, L. Sheu, J. Liu, B. Smart, C. J. Petzold, T.-y. Hsieh, A. Pitcher, J. D. Keasling, and C. R. Bertozzi Membrane proteomics of phagosomes suggests a connection to autophagy PNAS, November 4, 2008; 105(44): 16952 - 16957. [Abstract] [Full Text] [PDF]

				E. Bergeret, J. Perrin, M. Williams, D. Grunwald, E. Engel, D. Thevenon, E. Taillebourg, F. Bruckert, P. Cosson, and M.-O. Fauvarque TM9SF4 is required for Drosophila cellular immunity via cell adhesion and phagocytosis J. Cell Sci., October 15, 2008; 121(20): 3325 - 3334. [Abstract] [Full Text] [PDF]

				M. E. Hillenmeyer, E. Fung, J. Wildenhain, S. E. Pierce, S. Hoon, W. Lee, M. Proctor, R. P. St.Onge, M. Tyers, D. Koller, et al. The Chemical Genomic Portrait of Yeast: Uncovering a Phenotype for All Genes Science, April 18, 2008; 320(5874): 362 - 365. [Abstract] [Full Text] [PDF]

				M. Islinger, G. H. Luers, K. W. Li, M. Loos, and A. Volkl Rat Liver Peroxisomes after Fibrate Treatment: A SURVEY USING QUANTITATIVE MASS SPECTROMETRY J. Biol. Chem., August 10, 2007; 282(32): 23055 - 23069. [Abstract] [Full Text] [PDF]

				M. S. Goligorsky, F. Addabbo, and E. O'Riordan Diagnostic Potential of Urine Proteome: A Broken Mirror of Renal Diseases J. Am. Soc. Nephrol., August 1, 2007; 18(8): 2233 - 2239. [Abstract] [Full Text] [PDF]

				Y. Brunner, Y. Coute, M. Iezzi, M. Foti, M. Fukuda, D. F. Hochstrasser, C. B. Wollheim, and J.-C. Sanchez Proteomics Analysis of Insulin Secretory Granules Mol. Cell. Proteomics, June 1, 2007; 6(6): 1007 - 1017. [Abstract] [Full Text] [PDF]

				T. M. Casey, J. L. Meade, and E. W. Hewitt Organelle Proteomics: Identification of the Exocytic Machinery Associated with the Natural Killer Cell Secretory Lysosome Mol. Cell. Proteomics, May 1, 2007; 6(5): 767 - 780. [Abstract] [Full Text] [PDF]

				T. Stasyk, N. Schiefermeier, S. Skvortsov, H. Zwierzina, J. Peranen, G. K. Bonn, and L. A. Huber Identification of Endosomal Epidermal Growth Factor Receptor Signaling Targets by Functional Organelle Proteomics Mol. Cell. Proteomics, May 1, 2007; 6(5): 908 - 922. [Abstract] [Full Text] [PDF]

				L. Schaheen, G. Patton, and H. Fares Suppression of the cup-5 mucolipidosis type IV-related lysosomal dysfunction by the inactivation of an ABC transporter in C. elegans Development, October 1, 2006; 133(19): 3939 - 3948. [Abstract] [Full Text] [PDF]

				F. Tribl, M. Gerlach, K. Marcus, E. Asan, T. Tatschner, T. Arzberger, H. E. Meyer, G. Bringmann, and P. Riederer "Subcellular Proteomics" of Neuromelanin Granules Isolated from the Human Brain Mol. Cell. Proteomics, July 1, 2005; 4(7): 945 - 957. [Abstract] [Full Text] [PDF]