|
|
||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Molecular & Cellular Proteomics 4:133-143, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.
,
,
,¶,||,**
From the
Research Institute, The Hospital for Sick Children, Toronto M5G 1X8, Canada and the
Departments of Laboratory Medicine and Pathobiology, ¶ Biochemistry, and || Pediatrics, University of Toronto, Toronto, Ontario M5S 1A1, Canada
Lysosomes are endocytic subcellular compartments that contribute to the degradation and recycling of cellular material. Using highly purified rat liver tritosomes (Triton WR1339-filled lysosomes) and an ion exchange chromatography/LC-tandem MS-based protein/peptide separation and identification procedure, we characterized the major integral membrane protein complement of this organelle. While many of the 215 proteins we identified have been previously associated with lysosomes and endosomes, others have been associated with the endoplasmic reticulum, Golgi, cytosol, plasma membrane, and lipid rafts. At least 20 proteins were identified as unknown cDNAs that have no orthologues of known function, and 35 proteins were identified that function in protein and vesicle trafficking. This latter group includes multiple Rab and SNARE proteins as well as ubiquitin. Defining the roles of these proteins in the lysosomal membrane will assist in elucidating novel lysosomal functions involved in cellular homeostasis and pathways that are affected in various disease processes.
![]()
CiteULike
Complore
Connotea
Del.icio.us
Digg
Reddit
Technorati What's this?
This article has been cited by other articles:
![]() |
W. Shui, L. Sheu, J. Liu, B. Smart, C. J. Petzold, T.-y. Hsieh, A. Pitcher, J. D. Keasling, and C. R. Bertozzi Membrane proteomics of phagosomes suggests a connection to autophagy PNAS, November 4, 2008; 105(44): 16952 - 16957. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. Bergeret, J. Perrin, M. Williams, D. Grunwald, E. Engel, D. Thevenon, E. Taillebourg, F. Bruckert, P. Cosson, and M.-O. Fauvarque TM9SF4 is required for Drosophila cellular immunity via cell adhesion and phagocytosis J. Cell Sci., October 15, 2008; 121(20): 3325 - 3334. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. E. Hillenmeyer, E. Fung, J. Wildenhain, S. E. Pierce, S. Hoon, W. Lee, M. Proctor, R. P. St.Onge, M. Tyers, D. Koller, et al. The Chemical Genomic Portrait of Yeast: Uncovering a Phenotype for All Genes Science, April 18, 2008; 320(5874): 362 - 365. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. Islinger, G. H. Luers, K. W. Li, M. Loos, and A. Volkl Rat Liver Peroxisomes after Fibrate Treatment: A SURVEY USING QUANTITATIVE MASS SPECTROMETRY J. Biol. Chem., August 10, 2007; 282(32): 23055 - 23069. [Abstract] [Full Text] [PDF] |
||||
![]() |
M. S. Goligorsky, F. Addabbo, and E. O'Riordan Diagnostic Potential of Urine Proteome: A Broken Mirror of Renal Diseases J. Am. Soc. Nephrol., August 1, 2007; 18(8): 2233 - 2239. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Brunner, Y. Coute, M. Iezzi, M. Foti, M. Fukuda, D. F. Hochstrasser, C. B. Wollheim, and J.-C. Sanchez Proteomics Analysis of Insulin Secretory Granules Mol. Cell. Proteomics, June 1, 2007; 6(6): 1007 - 1017. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. M. Casey, J. L. Meade, and E. W. Hewitt Organelle Proteomics: Identification of the Exocytic Machinery Associated with the Natural Killer Cell Secretory Lysosome Mol. Cell. Proteomics, May 1, 2007; 6(5): 767 - 780. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Stasyk, N. Schiefermeier, S. Skvortsov, H. Zwierzina, J. Peranen, G. K. Bonn, and L. A. Huber Identification of Endosomal Epidermal Growth Factor Receptor Signaling Targets by Functional Organelle Proteomics Mol. Cell. Proteomics, May 1, 2007; 6(5): 908 - 922. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. Schaheen, G. Patton, and H. Fares Suppression of the cup-5 mucolipidosis type IV-related lysosomal dysfunction by the inactivation of an ABC transporter in C. elegans Development, October 1, 2006; 133(19): 3939 - 3948. [Abstract] [Full Text] [PDF] |
||||
![]() |
F. Tribl, M. Gerlach, K. Marcus, E. Asan, T. Tatschner, T. Arzberger, H. E. Meyer, G. Bringmann, and P. Riederer "Subcellular Proteomics" of Neuromelanin Granules Isolated from the Human Brain Mol. Cell. Proteomics, July 1, 2005; 4(7): 945 - 957. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Journal of Lipid Research | ASBMB Today |