HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M400152-MCP200v1 4/2/191    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Karsan, A. Articles by Veenstra, T. Search for Related Content PubMed PubMed Citation Articles by Karsan, A. Articles by Veenstra, T. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 4:191-204, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Quantitative Proteomic Analysis of Sokotrasterol Sulfate-stimulated Primary Human Endothelial Cells ^*,S

Aly Karsan^,,¶,, Ingrid Pollet, Li-Rong Yu^¶, King C. Chan^¶, Thomas P. Conrads^¶, David A. Lucas^¶, Raymond Andersen^|| and Timothy Veenstra^¶

From the Department of Medical Biophysics and Pathology and Laboratory Medicine, British Columbia Cancer Agency, Vancouver, British Columbia V5Z 1L3, Canada, ^¶ Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc., NCI, National Institutes of Health, Frederick, Maryland 21702-1201, and ^|| Department of Chemistry, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada

The endothelium forms a continuous monolayer at the interface between blood and tissue and contributes significantly to the sensing and transducing of signals between blood and tissue. New blood vessel formation, or angiogenesis, is initiated by the activation of endothelial cells and is an important process required for various pathological and physiological situations. This study used cleavable isotope-coded affinity tag reagents combined with mass spectrometry to investigate the molecular basis of a recently discovered angiogenesis-promoting steroid, sokotrasterol sulfate. Changes in the relative abundances of over 1000 proteins within human endothelial cells treated with sokotrasterol sulfate and vehicle-treated cells were identified and quantitated using this technique. A method that examines the entire ensemble of quantitative measurements was developed to identify proteins that showed a statistically significant change in relative abundance resulting from treatment with sokotrasterol sulfate. A total of 93 proteins was significantly up-regulated, and 37 were down-regulated in response to sokotrasterol sulfate stimulation of endothelial cells. Among the up-regulated proteins, several were identified that are novel to endothelial cells and are likely involved in cell communication and morphogenesis. These findings are consistent with a role for sokotrasterol sulfate in endothelial sprouting.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				I. Jorge, P. Navarro, P. Martinez-Acedo, E. Nunez, H. Serrano, A. Alfranca, J. M. Redondo, and J. Vazquez Statistical Model to Analyze Quantitative Proteomics Data Obtained by 18O/16O Labeling and Linear Ion Trap Mass Spectrometry: Application to the Study of Vascular Endothelial Growth Factor-induced Angiogenesis in Endothelial Cells Mol. Cell. Proteomics, May 1, 2009; 8(5): 1130 - 1149. [Abstract] [Full Text] [PDF]

				C. Roesli, V. Mumprecht, D. Neri, and M. Detmar Identification of the surface-accessible, lineage-specific vascular proteome by two-dimensional peptide mapping FASEB J, June 1, 2008; 22(6): 1933 - 1944. [Abstract] [Full Text] [PDF]

				F. Bussolino Small Molecule Approaches for Promoting Ischemic Tissue Vascularization Circ. Res., August 4, 2006; 99(3): 231 - 233. [Full Text] [PDF]

				S. Murphy, B. Larrivee, I. Pollet, K. S. Craig, D. E. Williams, X.-H. Huang, M. Abbott, F. Wong, C. Curtis, T. P. Conrads, et al. Identification of Sokotrasterol Sulfate As a Novel Proangiogenic Steroid Circ. Res., August 4, 2006; 99(3): 257 - 265. [Abstract] [Full Text] [PDF]