Advertisement
MCP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M400195-MCP200 on January 18, 2005.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M400195-MCP200v1
4/3/300    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Glossary
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Park, S. W.
Right arrow Articles by Wei, L.-N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Park, S. W.
Right arrow Articles by Wei, L.-N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 4:300-309, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Tyrosine Nitration on p65

A Novel Mechanism to Rapidly Inactivate Nuclear Factor-{kappa}B*

Sung Wook Park, M. D.Mostaqul Huq, Xinli Hu and Li-Na Wei{ddagger}

From the Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota 55455

NO is an important factor that induces post-translational modifications of proteins by cellular reduction and oxidation mechanism: cysteinyl-nitrosylation or Tyr nitration. Nuclear factor (NF)-{kappa}B activity can be rapidly suppressed by sodium nitroprusside, a NO donor. This effect was effectively reversed by peroxynitrite scavenger deferoxamine, suggesting a Tyr nitration-mediated mechanism. Western blot with nitrotyrosine-specific antibody demonstrated that the p65 subunit of NF-{kappa}B was predominantly nitrated on Tyr residues. Tyr nitration of p65 induced its dissociation from p50, its association with I{kappa}B{alpha}, and subsequent sequestration of p65 in the cytoplasm by I{kappa}B{alpha}-mediated export. Liquid chromatography-coupled nanoelectrospray mass spectrometry revealed specific nitration on Tyr-66 and Tyr-152 residues of p65. Mutation studies confirmed that both Tyr-66 and Tyr-152 residues were important for the direct effects of NO on p65, which resulted in more p65 export and inactivation of NF-{kappa}B activity. This study identified a novel and efficient pathway where NO rapidly inactivated NF-{kappa}B activity by inducing Tyr nitration on p65.

{ddagger} To whom correspondence should be addressed: Dept. of Pharmacology, University of Minnesota, 6-120 Jackson Hall, 321 Church St. S E., Minneapolis, MN 55455. Tel.: 612-625-9402; Fax: 612-625-8408; E-mail: weixx009{at}umn.edu


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 ChestHome page
G. O. Osoata, T. Hanazawa, C. Brindicci, M. Ito, P. J. Barnes, S. Kharitonov, and K. Ito
Peroxynitrite Elevation in Exhaled Breath Condensate of COPD and Its Inhibition by Fudosteine
Chest, June 1, 2009; 135(6): 1513 - 1520.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
P. Pacher, J. S. Beckman, and L. Liaudet
Nitric Oxide and Peroxynitrite in Health and Disease
Physiol Rev, January 1, 2007; 87(1): 315 - 424.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Ishikawa and P. L. Morris
Interleukin-1{beta} Signals through a c-Jun N-Terminal Kinase-Dependent Inducible Nitric Oxide Synthase and Nitric Oxide Production Pathway in Sertoli Epithelial Cells
Endocrinology, November 1, 2006; 147(11): 5424 - 5430.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
R.P. Webster, D. Brockman, and L. Myatt
Nitration of p38 MAPK in the placenta: association of nitration with reduced catalytic activity of p38 MAPK in pre-eclampsia
Mol. Hum. Reprod., November 1, 2006; 12(11): 677 - 685.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Zheng, X. Yu, P. Collin-Osdoby, and P. Osdoby
RANKL Stimulates Inducible Nitric-oxide Synthase Expression and Nitric Oxide Production in Developing Osteoclasts: AN AUTOCRINE NEGATIVE FEEDBACK MECHANISM TRIGGERED BY RANKL-INDUCED INTERFERON-beta VIA NF-{kappa}B THAT RESTRAINS OSTEOCLASTOGENESIS AND BONE RESORPTION
J. Biol. Chem., June 9, 2006; 281(23): 15809 - 15820.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
K. S. Farley, L. F. Wang, H. M. Razavi, C. Law, M. Rohan, D. G. McCormack, and S. Mehta
Effects of macrophage inducible nitric oxide synthase in murine septic lung injury
Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1164 - L1172.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
S. K. Biswas and J. B. Lopes de Faria
Does peroxynitrite sustain nuclear factor-{kappa}B?
Cardiovasc Res, September 1, 2005; 67(4): 745 - 746.
[Full Text] [PDF]

Home page
 Cardiovasc ResHome page
S. S. Gross and Y. Hattori
Room for both tyrosine nitration and cysteine nitrosylation to regulate NF{kappa}B activity, but perhaps only one modification at a time
Cardiovasc Res, September 1, 2005; 67(4): 747 - 748.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement