HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: R500006-MCP200v1 4/4/394    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Caldwell, R. L. Articles by Caprioli, R. M. Search for Related Content PubMed PubMed Citation Articles by Caldwell, R. L. Articles by Caprioli, R. M.

---

Review

Tissue Profiling by Mass Spectrometry

A Review of Methodology and Applications^*

Robert L. Caldwell and Richard M. Caprioli

Mass Spectrometry Research Center, Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232-8575

Matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) has become a valuable tool to address a broad range of questions in many areas of biomedical research. One such application allows spectra to be obtained directly from intact tissues, termed "profiling" (low resolution) and "imaging" (high resolution). In light of the fact that MALDI tissue profiling allows over a thousand peptides and proteins to be rapidly detected from a variety of tissues, its application to disease processes is of special interest. For example, protein profiles from tumors may allow accurate prediction of tumor behavior, diagnosis, and prognosis and uncover etiologies underlying idiopathic diseases. MALDI MS, in conjunction with laser capture microdissection, is able to produce protein expression profiles from a relatively small number of cells from specific regions of heterogeneous tissue architectures. Imaging mass spectrometry enables the investigator to assess the spatial distribution of proteins, drugs, and their metabolites in intact tissues. This article provides an overview of several tissue profiling and imaging applications performed by MALDI MS, including sample preparation, matrix selection and application, histological staining prior to MALDI analysis, tissue profiling, imaging, and data analysis. Several applications represent direct translation of this technology to clinically relevant problems.

This article has been cited by other articles:

				N. Grabe, T. Pommerencke, T. Steinberg, H. Dickhaus, and P. Tomakidi Reconstructing protein networks of epithelial differentiation from histological sections Bioinformatics, December 1, 2007; 23(23): 3200 - 3208. [Abstract] [Full Text] [PDF]

				T. A. Drake and P. Ping Thematic review series: Systems Biology Approaches to Metabolic and Cardiovascular Disorders. Proteomics approaches to the systems biology of cardiovascular diseases J. Lipid Res., January 1, 2007; 48(1): 1 - 8. [Abstract] [Full Text] [PDF]

				D. S. Cornett, J. A. Mobley, E. C. Dias, M. Andersson, C. L. Arteaga, M. E. Sanders, and R. M. Caprioli A Novel Histology-directed Strategy for MALDI-MS Tissue Profiling That Improves Throughput and Cellular Specificity in Human Breast Cancer Mol. Cell. Proteomics, October 1, 2006; 5(10): 1975 - 1983. [Abstract] [Full Text] [PDF]

				M. Sanchez-Carbayo Antibody Arrays: Technical Considerations and Clinical Applications in Cancer Clin. Chem., September 1, 2006; 52(9): 1651 - 1659. [Abstract] [Full Text] [PDF]

				J. Han and K. L. Schey MALDI Tissue Imaging of Ocular Lens {alpha}-Crystallin. Invest. Ophthalmol. Vis. Sci., July 1, 2006; 47(7): 2990 - 2996. [Abstract] [Full Text] [PDF]

				M. W. Duncan and S. W. Hunsucker Proteomics as a Tool for Clinically Relevant Biomarker Discovery and Validation Experimental Biology and Medicine, December 1, 2005; 230(11): 808 - 817. [Abstract] [Full Text] [PDF]