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Originally published In Press as doi:10.1074/mcp.R500005-MCP200 on March 1, 2005.
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Molecular & Cellular Proteomics 4:419-434, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

Review

Statistical and Computational Methods for Comparative Proteomic Profiling Using Liquid Chromatography-Tandem Mass Spectrometry

Jennifer Listgarten{ddagger} and Andrew Emili§,¶,||

From the {ddagger} Department of Computer Science, University of Toronto, Toronto, Ontario M5S 3G4, Canada; § Program in Proteomics and Bioinformatics and Banting and Best Department of Medical Research, University of Toronto, Toronto, Ontario M5G 1L6, Canada

The combined method of LC-MS/MS is increasingly being used to explore differences in the proteomic composition of complex biological systems. The reliability and utility of such comparative protein expression profiling studies is critically dependent on an accurate and rigorous assessment of quantitative changes in the relative abundance of the myriad of proteins typically present in a biological sample such as blood or tissue. In this review, we provide an overview of key statistical and computational issues relevant to bottom-up shotgun global proteomic analysis, with an emphasis on methods that can be applied to improve the dependability of biological inferences drawn from large proteomic datasets. Focusing on a start-to-finish approach, we address the following topics: 1) low-level data processing steps, such as formation of a data matrix, filtering, and baseline subtraction to minimize noise, 2) mid-level processing steps, such as data normalization, alignment in time, peak detection, peak quantification, peak matching, and error models, to facilitate profile comparisons; and, 3) high-level processing steps such as sample classification and biomarker discovery, and related topics such as significance testing, multiple testing, and choice of feature space. We report on approaches that have recently been developed for these steps, discussing their merits and limitations, and propose areas deserving of further research.

|| To whom correspondence should be addressed: CH Best Institute, 112 College St., Toronto, Ontario M5G 1L6, Canada. Tel.: 416-946-7281; Fax: 416-978-8528; E-mail: andrew.emili{at}utoronto.ca


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