HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M500030-MCP200v1 4/4/492    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Celis, J. E. Articles by Gromova, I. Search for Related Content PubMed PubMed Citation Articles by Celis, J. E. Articles by Gromova, I. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 4:492-522, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Identification of Extracellular and Intracellular Signaling Components of the Mammary Adipose Tissue and Its Interstitial Fluid in High Risk Breast Cancer Patients

Toward Dissecting The Molecular Circuitry of Epithelial-Adipocyte Stromal Cell Interactions^*

Julio E. Celis^,,¶, José M. A. Moreira^,, Teresa Cabezón^,, Pavel Gromov^,, Esbern Friis^,||, Fritz Rank^,** and Irina Gromova^,

From the Department of Proteomics in Cancer, Institute of Cancer Biology and Danish Centre for Translational Breast Cancer Research, Danish Cancer Society and the ^|| Department of Breast and Endocrine Surgery and ^** Department of Pathology, The Centre of Diagnostic Investigations, Copenhagen University Hospital, DK-2100 Copenhagen, Denmark

It has become clear that growth and progression of breast tumor cells not only depend on their malignant potential but also on factors present in the tumor microenvironment. Of the cell types that constitute the mammary stroma, the adipocytes are perhaps the least well studied despite the fact that they represent one of the most prominent cell types surrounding the breast tumor cells. There is compelling evidence demonstrating a role for the mammary fat pad in mammary gland development, and some studies have revealed the ability of fat tissue to augment the growth and ability to metastasize of mammary carcinoma cells. Very little is known, however, about which factors adipocytes produce that may orchestrate these actions and how this may come about. In an effort to shed some light on these questions, we present here a detailed proteomic analysis, using two-dimensional gel-based technology, mass spectrometry, immunoblotting, and antibody arrays, of adipose cells and interstitial fluid of fresh fat tissue samples collected from sites topologically distant from the tumors of high risk breast cancer patients that underwent mastectomy and that were not treated prior to surgery. A total of 359 unique proteins were identified, including numerous signaling molecules, hormones, cytokines, and growth factors, involved in a variety of biological processes such as signal transduction and cell communication; energy metabolism; protein metabolism; cell growth and/or maintenance; immune response; transport; regulation of nucleobase, nucleoside, and nucleic acid metabolism; and apoptosis. Apart from providing a comprehensive overview of the mammary fat proteome and its interstitial fluid, the results offer some insight as to the role of adipocytes in the breast tumor microenvironment and provide a first glance of their molecular cellular circuitry. In addition, the results open new possibilities to the study of obesity, which has a strong association with type 2 diabetes, hypertension, and coronary heart disease.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. E. Celis, P. Gromov, T. Cabezon, J. M. A. Moreira, E. Friis, K. Jirstrom, A. Llombart-Bosch, V. Timmermans-Wielenga, F. Rank, and I. Gromova 15-Prostaglandin Dehydrogenase Expression Alone or in Combination with ACSM1 Defines a Subgroup of the Apocrine Molecular Subtype of Breast Carcinoma Mol. Cell. Proteomics, October 1, 2008; 7(10): 1795 - 1809. [Abstract] [Full Text] [PDF]

				S. M. Hauck, C. J. Gloeckner, M. E. Harley, S. Schoeffmann, K. Boldt, P. A. R. Ekstrom, and M. Ueffing Identification of Paracrine Neuroprotective Candidate Proteins by a Functional Assay-driven Proteomics Approach Mol. Cell. Proteomics, July 1, 2008; 7(7): 1349 - 1361. [Abstract] [Full Text] [PDF]

				M. Corton, J. I. Botella-Carretero, J. A. Lopez, E. Camafeita, J. L. San Millan, H. F. Escobar-Morreale, and B. Peral Proteomic analysis of human omental adipose tissue in the polycystic ovary syndrome using two-dimensional difference gel electrophoresis and mass spectrometry Hum. Reprod., March 1, 2008; 23(3): 651 - 661. [Abstract] [Full Text] [PDF]

				O. Gul, H. Basaga, and O. Kutuk Apoptotic blocks and chemotherapy resistance: strategies to identify Bcl-2 protein signatures Brief Funct Genomic Proteomic, February 18, 2008; (2008) eln002v1. [Abstract] [Full Text] [PDF]

				J. M. Gimble, A. J. Katz, and B. A. Bunnell Adipose-Derived Stem Cells for Regenerative Medicine Circ. Res., May 11, 2007; 100(9): 1249 - 1260. [Abstract] [Full Text] [PDF]

				G. Alvarez-Llamas, E. Szalowska, M. P. de Vries, D. Weening, K. Landman, A. Hoek, B. H. R. Wolffenbuttel, H. Roelofsen, and R. J. Vonk Characterization of the Human Visceral Adipose Tissue Secretome Mol. Cell. Proteomics, April 1, 2007; 6(4): 589 - 600. [Abstract] [Full Text] [PDF]

				S. Zvonic, M. Lefevre, G. Kilroy, Z. E. Floyd, J. P. DeLany, I. Kheterpal, A. Gravois, R. Dow, A. White, X. Wu, et al. Secretome of Primary Cultures of Human Adipose-derived Stem Cells: Modulation of Serpins by Adipogenesis Mol. Cell. Proteomics, January 1, 2007; 6(1): 18 - 28. [Abstract] [Full Text] [PDF]

				F. Bertucci, D. Birnbaum, and A. Goncalves Proteomics of Breast Cancer: Principles and Potential Clinical Applications Mol. Cell. Proteomics, October 1, 2006; 5(10): 1772 - 1786. [Abstract] [Full Text] [PDF]

				S. de Assis, M. Wang, S. Goel, A. Foxworth, W. Helferich, and L. Hilakivi-Clarke Excessive Weight Gain during Pregnancy Increases Carcinogen-Induced Mammary Tumorigenesis in Sprague-Dawley and Lean and Obese Zucker Rats J. Nutr., April 1, 2006; 136(4): 998 - 1004. [Abstract] [Full Text] [PDF]