HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M500017-MCP200v1 4/4/570    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ohlsson, G. Articles by Celis, J. E. Search for Related Content PubMed PubMed Citation Articles by Ohlsson, G. Articles by Celis, J. E. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 4:570-581, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Loss of Expression of the Adipocyte-type Fatty Acid-binding Protein (A-FABP) Is Associated with Progression of Human Urothelial Carcinomas^*

Gita Ohlsson^,, José M. A. Moreira, Pavel Gromov, Guido Sauter^¶ and Julio E. Celis^,

From the Department of Proteomics in Cancer, Institute of Cancer Biology, Danish Cancer Society, Copenhagen, Denmark DK-2100; and ^¶ Division of Molecular Pathology, Institute of Pathology, University of Basel, Basel, Switzerland 4031

Bladder cancer is the fifth most common malignancy in the world and represents the second most common cause of death among genitourinary tumors. Current prognostic parameters such as grade and stage cannot predict with certainty the long-term outcome of bladder cancer, and as a result there is a pressing need to identify markers that may predict tumor behavior. Earlier we identified the adipocyte fatty acid-binding protein (A-FABP), a small-molecular-mass fatty acid-binding protein that functions by facilitating the intracellular diffusion of fatty acids between cellular compartments as a putative marker of progression based on a limited study of fresh bladder urothelial carcinomas (UCs) (Celis, J. E., Ostergaard, M., Basse, B., Celis, A., Lauridsen, J. B., Ratz, G. P., Andersen, I., Hein, B., Wolf, H., Orntoft, T. F., and Rasmussen, H. H. (1996) Loss of adipocyte-type fatty acid binding protein and other protein biomarkers is associated with progression of human bladder transitional cell carcinomas. Cancer Res.56, 4782–4790). Here we have comprehensively examined the protein expression profiles of a much larger sample set consisting of 153 bladder specimens (46 nonmalignant biopsies, 11 pTa G1, 40 pTa G2, 10 pTa G3, 13 pT1 G3, 23 pT2-4 G3, and 10 pT2-4 G4) by gel-based proteomics in combination with immunohistochemistry (IHC) using a peptide-based rabbit polyclonal antibody that reacts specifically with this protein. Proteomic profiling showed a striking down-regulation of A-FABP in invasive lesions, a fact that correlated well with immunohistochemical analysis of the same samples. The IHC results were confirmed by using a tissue microarray (TMA) containing 2,317 samples derived from 1,849 bladder cancer patients. Moreover, we found that the altered expression of A-FABP in invasive UCs is not due to deregulated expression of peroxisome proliferator-activated receptor (PPAR), a trans-activator of A-FABP. Taken together, these results provide evidence that deregulation of A-FABP may play a role in bladder cancer progression and suggest that A-FABP could have a significant prognostic value in combination with other biomarkers.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				J. M. A. Moreira, G. Ohlsson, P. Gromov, R. Simon, G. Sauter, J. E. Celis, and I. Gromova Bladder Cancer-associated Protein, a Potential Prognostic Biomarker in Human Bladder Cancer Mol. Cell. Proteomics, January 1, 2010; 9(1): 161 - 177. [Abstract] [Full Text] [PDF]

				E. Schiffer, A. Vlahou, A. Petrolekas, K. Stravodimos, R. Tauber, J. E. Geschwend, J. Neuhaus, J.-U. Stolzenburg, M. R. Conaway, H. Mischak, et al. Prediction of Muscle-invasive Bladder Cancer Using Urinary Proteomics Clin. Cancer Res., August 1, 2009; 15(15): 4935 - 4943. [Abstract] [Full Text] [PDF]

				N. N. Raveendran, K. Silver, L. C. Freeman, D. Narvaez, K. Weng, S. Ganta, and J. D. Lillich Drug-Induced Alterations to Gene and Protein Expression in Intestinal Epithelial Cell 6 Cells Suggest a Role for Calpains in the Gastrointestinal Toxicity of Nonsteroidal Anti-Inflammatory Agents J. Pharmacol. Exp. Ther., May 1, 2008; 325(2): 389 - 399. [Abstract] [Full Text] [PDF]

				T. Pisitkun, R. Johnstone, and M. A. Knepper Discovery of Urinary Biomarkers Mol. Cell. Proteomics, October 1, 2006; 5(10): 1760 - 1771. [Abstract] [Full Text] [PDF]