MCP Waters-The Science of What's Possible
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M400176-MCP200 on January 24, 2005.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M400176-MCP200v1
4/4/582    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vincensini, L.
Right arrow Articles by Braun Breton, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vincensini, L.
Right arrow Articles by Braun Breton, C.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 4:582-593, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Proteomic Analysis Identifies Novel Proteins of the Maurer’s Clefts, a Secretory Compartment Delivering Plasmodium falciparum Proteins to the Surface of Its Host Cell*,S

Laetitia Vincensini{ddagger},§, Sophie Richert,||, Thierry Blisnick{ddagger}, Alain Van Dorsselaer, Emmanuelle Leize-Wagner, Thierry Rabilloud** and Catherine Braun Breton{ddagger},{ddagger}{ddagger},§§

From the {ddagger} Unité de Biologie des Interactions Hôte-Parasite, CNRS URA 2581, Institut Pasteur, 25–28 Rue du Dr Roux, 75724 Paris Cedex 15, the Laboratoire de Spectrométrie de Masse Bio-Organique, 25 rue Becquerel, 67087 Strasbourg Cedex 2, the ** Laboratoire de Bioénergétique Cellulaire et Pathologique, Commissariat à l’Energie Atomique Grenoble, 17 rue des martyrs, 38054 Grenoble Cedex 9, and {ddagger}{ddagger} UMR 5539 CNRS-Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France

A novel method was validated for the efficient distinction between malaria parasite-derived and host cell proteins in mass spectrometry analyses. This method was applied to a ghost fraction from Plasmodium falciparum-infected erythrocytes containing the red blood cell plasma membrane, the erythrocyte submembrane skeleton, and the Maurer’s clefts, a Golgi-like apparatus linked to and addressing parasite proteins to the host cell surface. This method allowed the identification of 78 parasite proteins. Among these we identified seven novel proteins of the Maurer’s clefts based on immunofluorescence studies and proteinase K digestion assays. The products of six contiguous genes located on chromosome 5 were identified, and the location within the Maurer’s clefts was established for two of them. This suggests a clustering of genes encoding Maurer’s cleft proteins. Our study sheds new light on the biological function of the Maurer’s clefts, which are central to the pathogenesis and to the intraerythrocytic development of P. falciparum.

§§ To whom correspondence should be addressed. Tel.: 33-4-67-14-33-81; Fax: 33-4-67-14-42-86; E-mail: cbb{at}univ-montp2.fr


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
S. E. Francis, V. A. Malkov, A. V. Oleinikov, E. Rossnagle, J. P. Wendler, T. K. Mutabingwa, M. Fried, and P. E. Duffy
Six Genes Are Preferentially Transcribed by the Circulating and Sequestered Forms of Plasmodium falciparum Parasites That Infect Pregnant Women
Infect. Immun., October 1, 2007; 75(10): 4838 - 4850.
[Abstract] [Full Text] [PDF]

Home page
 Protein Sci.Home page
A. Shonhai, A. Boshoff, and G. L. Blatch
The structural and functional diversity of Hsp70 proteins from Plasmodium falciparum
Protein Sci., September 1, 2007; 16(9): 1803 - 1818.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
T. Spielmann, P. L. Hawthorne, M. W.A. Dixon, M. Hannemann, K. Klotz, D. J. Kemp, N. Klonis, L. Tilley, K. R. Trenholme, and D. L. Gardiner
A Cluster of Ring Stage-specific Genes Linked to a Locus Implicated in Cytoadherence in Plasmodium falciparum Codes for PEXEL-negative and PEXEL-positive Proteins Exported into the Host Cell
Mol. Biol. Cell, August 1, 2006; 17(8): 3613 - 3624.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Spycher, M. Rug, N. Klonis, D. J. P. Ferguson, A. F. Cowman, H.-P. Beck, and L. Tilley
Genesis of and Trafficking to the Maurer's Clefts of Plasmodium falciparum-Infected Erythrocytes.
Mol. Cell. Biol., June 1, 2006; 26(11): 4074 - 4085.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
B. M. Cooke, D. W. Buckingham, F. K. Glenister, K. M. Fernandez, L. H. Bannister, M. Marti, N. Mohandas, and R. L. Coppel
A Maurer's cleft-associated protein is essential for expression of the major malaria virulence antigen on the surface of infected red blood cells
J. Cell Biol., March 13, 2006; 172(6): 899 - 908.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Marchesini, M. Vieira, S. Luo, S. N. J. Moreno, and R. Docampo
A Malaria Parasite-encoded Vacuolar H+-ATPase Is Targeted to the Host Erythrocyte
J. Biol. Chem., November 4, 2005; 280(44): 36841 - 36847.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.