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Originally published In Press as doi:10.1074/mcp.M400077-MCP200 on January 19, 2005.
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Molecular & Cellular Proteomics 4:721-730, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Phosphotyrosine Proteomic Study of Interferon {alpha} Signaling Pathway Using a Combination of Immunoprecipitation and Immobilized Metal Affinity Chromatography*

Haiyan Zheng{ddagger}, Ping Hu{ddagger},§, Douglas F. Quinn{ddagger} and Y. Karen Wang{ddagger},¶

From {ddagger} Discovery Technologies, Novartis Institute for Biomedical Research, Inc., Cambridge, MA 02139

Tyrosine phosphorylation is a type of post-translational modification that plays a crucial role in signal transduction. Thus, the study of this modification at the proteomic level has great biological significance. However, because of the low abundance of tyrosine-phosphorylated proteins in total cell lysate, it is difficult to evaluate the dynamics of tyrosine phosphorylation at a global level. In this work, proteins carrying phosphotyrosine (pTyr) were first purified from whole cell lysate by immunoprecipitation using anti-pTyr monoclonal antibodies. After tryptic digestion, phosphopeptides were further enriched by IMAC and analyzed by LC-MS. Quantitative changes of tyrosine phosphorylation at the global level were evaluated using isotopic labeling (introduced at the methyl esterification step prior to IMAC). Using this double enrichment approach, we characterized interferon {alpha} (IFN{alpha})-induced pTyr proteomic changes in Jurkat cells. We observed induced phosphorylation on several well documented as well as novel tyrosine phosphorylation sites on proteins involved in IFN{alpha} signal transduction, such as Tyk2, JAK1, and IFNAR subunits. A specific site on {alpha}-tubulin (Tyr-271) was observed to be phosphorylated upon treatment as well. Furthermore, our results suggest that LOC257106, a CDC42 GAP-like protein, is potentially involved in this pathway.

To whom correspondence should be addressed: Novartis Institute for Biomedical Research, Inc., 250 Massachusetts Ave., Cambridge, MA 02139. Fax: 617-871-4086; E-mail: karen.wang{at}novartis.com


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