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Originally published In Press as doi:10.1074/mcp.R400010-MCP200 on April 22, 2005.
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Molecular & Cellular Proteomics 4:857-872, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

Review

Metabolic Labeling of Proteins for Proteomics*

Robert J. Beynon{ddagger} and Julie M. Pratt

From the Protein Function Group, Faculty of Veterinary Science, University of Liverpool, Crown Street, Liverpool L69 7ZJ, United Kingdom

Realization of the advantages of stable isotope labeling for proteomics has emerged gradually. However, many stable isotope label approaches rely on labeling in vitro using complex and sometimes expensive reagents. This review discusses strategies for labeling protein in vivo through metabolic incorporation of label into protein. This approach has many advantages, is particularly suited to single cells grown in culture (prokaryotic or eukaryotic), but is nonetheless subject to a number of complicating factors that must be controlled so that meaningful experiments can be conducted. Confounding issues include the metabolic lability of the amino acid precursor, incomplete labeling, and the role of protein turnover in labeling kinetics. All of these are controllable, provided that appropriate precautions are adopted.

{ddagger} To whom correspondence should be addressed. Tel.: 44-151-794-4312; Fax: 44-151-794-4243; E-mail: r.beynon{at}liv.ac.uk


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