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Molecular & Cellular Proteomics 4:902-913, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

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Research

Quantitative Analysis of Severe Acute Respiratory Syndrome (SARS)-associated Coronavirus-infected Cells Using Proteomic Approaches

Implications for Cellular Responses to Virus Infection^*,S

Xiao-Sheng Jiang, Liu-Ya Tang, Jie Dai, Hu Zhou, Su-Jun Li, Qi-Chang Xia, Jia-Rui Wu^,,¶ and Rong Zeng^,||

From the Research Center for Proteome Analysis, Key Lab of Proteomics, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Road, Shanghai 200031, China

We present the first proteomic analysis on the cellular response to severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. The differential proteomes of Vero E6 cells with and without infection of the SARS-CoV were resolved and quantitated with two-dimensional differential gel electrophoresis followed by ESI-MS/MS identification. Moreover isotope-coded affinity tag technology coupled with two-dimensional LC-MS/MS were also applied to the differential proteins of infected cells. By combining these two complementary strategies, 355 unique proteins were identified and quantitated with 186 of them differentially expressed (at least 1.5-fold quantitative alteration) between infected and uninfected Vero E6 cells. The implication for cellular responses to virus infection was analyzed in depth according to the proteomic results. Thus, the present work provides large scale protein-related information to investigate the mechanism of SARS-CoV infection and pathogenesis.

^|| To whom correspondence may be addressed: Research Center for Proteome Analysis, Inst. of Biochemistry and Cell Biology, Shanghai Insts. for Biological Sciences, Chinese Academy of Sciences, 320 YueYang Rd., Shanghai 200031, China. Tel.: 86-21-54920170; Fax: 86-21-54920171; E-mail: zr{at}sibs.ac.cn

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