HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M400177-MCP200v1 4/7/914    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, Z. Articles by Strauss, J. F. Search for Related Content PubMed PubMed Citation Articles by Zhang, Z. Articles by Strauss, J. F., III Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 4:914-923, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Dissecting the Axoneme Interactome

The Mammalian Orthologue of Chlamydomonas PF6 Interacts with Sperm-Associated Antigen 6, The Mammalian Orthologue of Chlamydomonas PF16^*

Zhibing Zhang, Brian H. Jones, Waixing Tang, Stuart B. Moss, Zhangyong Wei, Clement Ho, Michael Pollack^¶, Eran Horowitz, Jean Bennett, Michael E. Baker^|| and Jerome F. Strauss, III^,

From the Center for Research on Reproduction and Women’s Health and the Departments of Ophthalmology and ^¶ Pharmacology, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104 and the ^|| Department of Medicine, University of California, San Diego, California 92093

The axoneme central apparatus is thought to control flagellar/ciliary waveform and maintain the structural integrity of the axoneme, but proteins involved in these processes have not been fully elucidated. Moreover the network of interactions among them that allows these events to take place in a compact space has not been defined. PF6, a component of the Chlamydomonas central apparatus, is localized to the 1a projection of the C1 microtubule. Mutations in the Chlamydomonas PF6 gene result in flagellar paralysis. We characterized human and murine orthologues of PF6. The murine Pf6 gene is expressed in a pattern consistent with a role in flagella and cilia, and the PF6 protein is indeed localized to the central apparatus of the sperm flagellar axoneme. We discovered that a portion of PF6 associates with the mammalian orthologue of Chlamydomonas PF16 (sperm-associated antigen 6 (SPAG6)), another central apparatus protein that is localized to the C1 microtubule in algae. A fragment of PF6 corresponding to the PF6 domain that interacts with SPAG6 in yeast two-hybrid assays and colocalizes with SPAG6 in transfected cells was missing from epididymal sperm of SPAG6-deficient mice. SPAG6 binds to the mammalian orthologue of PF20, which in Chlamydomonas is located in bridges connecting the C2 and C1 microtubules. Thus, PF6, SPAG6, and PF20 form a newly identified network that links together components of the axoneme central apparatus and presumably participates in its dynamic regulation of ciliary and flagellar beat.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. S. Huh, M. A. M. Todd, and D. J. Picketts SCO-ping Out the Mechanisms Underlying the Etiology of Hydrocephalus Physiology, April 1, 2009; 24(2): 117 - 126. [Abstract] [Full Text] [PDF]

				Z. Zhang, M. A Zariwala, M. M Mahadevan, P. Caballero-Campo, X. Shen, E. Escudier, B. Duriez, A.-M. Bridoux, M. Leigh, G. L Gerton, et al. A Heterozygous Mutation Disrupting the SPAG16 Gene Results in Biochemical Instability of Central Apparatus Components of the Human Sperm Axoneme Biol Reprod, November 1, 2007; 77(5): 864 - 871. [Abstract] [Full Text] [PDF]

				D. Escalier Knockout mouse models of sperm flagellum anomalies Hum. Reprod. Update, July 1, 2006; 12(4): 449 - 461. [Abstract] [Full Text] [PDF]

				C. P. Ponting A novel domain suggests a ciliary function for ASPM, a brain size determining gene Bioinformatics, May 1, 2006; 22(9): 1031 - 1035. [Abstract] [Full Text] [PDF]

				Z. Zhang, I. Kostetskii, W. Tang, L. Haig-Ladewig, R. Sapiro, Z. Wei, A. M. Patel, J. Bennett, G. L. Gerton, S. B. Moss, et al. Deficiency of SPAG16L Causes Male Infertility Associated with Impaired Sperm Motility Biol Reprod, April 1, 2006; 74(4): 751 - 759. [Abstract] [Full Text] [PDF]

				M. J. Wargo, E. E. Dymek, and E. F. Smith Calmodulin and PF6 are components of a complex that localizes to the C1 microtubule of the flagellar central apparatus J. Cell Sci., October 15, 2005; 118(20): 4655 - 4665. [Abstract] [Full Text] [PDF]