Advertisement
MCP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M400193-MCP200 on April 22, 2005.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M400193-MCP200v1
4/7/924    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Glossary
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Unwin, R. D.
Right arrow Articles by Whetton, A. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Unwin, R. D.
Right arrow Articles by Whetton, A. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 4:924-935, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Quantitative Proteomic Analysis Using Isobaric Protein Tags Enables Rapid Comparison of Changes in Transcript and Protein Levels in Transformed Cells*,S

Richard D. Unwin{ddagger},§, Andrew Pierce{ddagger}, Rod B. Watson, David W. Sternberg|| and Anthony D. Whetton{ddagger},§,{ddagger}{ddagger}

From the {ddagger} Department of Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Withington, Manchester, M20 9BX, United Kingdom, § Mass Spectrometry Laboratory, Paterson Institute for Cancer Research, Christie Hospital, Withington, Manchester, M20 9BX, United Kingdom, Applied Biosystems Inc., Lingley House, 120 Birchwood Boulevard, Warrington, Cheshire, WA3 7QH, United Kingdom, and || Mount Sinai Medical School, One Gustave L. Levy Place, New York, New York 10029

Isobaric tags for relative and absolute quantitation, an approach to concurrent, relative quantification of proteins present in four cell preparations, have recently been described. To validate this approach using complex mammalian cell samples that show subtle differences in protein levels, a model stem cell-like cell line (FDCP-mix) in the presence or absence of the leukemogenic oncogene TEL/PDGFRß has been studied. Cell lysates were proteolytically digested, and peptides within each sample were labeled with one of four isobaric, isotope-coded tags via their N-terminal and/or lysine side chains. The four labeled samples are mixed and peptides separated by two-dimensional liquid chromatography online to a mass spectrometer (LC-MS). Upon peptide fragmentation, each tag releases a distinct mass reporter ion; the ratio of the four reporters therefore gives relative abundances of the given peptide. Relative quantification of proteins is derived using summed data from a number of peptides. TEL/PDGFRß leukemic oncogene-mediated changes in protein levels were compared with those seen in microarray analysis of control and transfected FDCP-mix cells. Changes at the protein level in most cases reflected those seen at the transcriptome level. Nonetheless, novel differences in protein expression were found that indicate potential mechanisms for effects of this oncogene.

{ddagger}{ddagger} To whom correspondence should be addressed. Tel.: 0161-306-4182; Fax: 0161-236-0409; E-mail: awhetton{at}picr.man.ac.uk


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Wu, K. Moissoglu, H. Wang, X. Wang, H. F. Frierson, M. A. Schwartz, and D. Theodorescu
Src phosphorylation of RhoGDI2 regulates its metastasis suppressor function
PNAS, April 7, 2009; 106(14): 5807 - 5812.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
Y. Wang, C. Mulligan, G. Denyer, F. Delom, F. Dagna-Bricarelli, V. L. J. Tybulewicz, E. M. C. Fisher, W. J. Griffiths, D. Nizetic, and J. Groet
Quantitative Proteomics Characterization of a Mouse Embryonic Stem Cell Model of Down Syndrome
Mol. Cell. Proteomics, April 1, 2009; 8(4): 585 - 595.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
M. Zhu, S. Dai, S. McClung, X. Yan, and S. Chen
Functional Differentiation of Brassica napus Guard Cells and Mesophyll Cells Revealed by Comparative Proteomics
Mol. Cell. Proteomics, April 1, 2009; 8(4): 752 - 766.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
E. Szajli, T. Feher, and K. F. Medzihradszky
Investigating the Quantitative Nature of MALDI-TOF MS
Mol. Cell. Proteomics, December 1, 2008; 7(12): 2410 - 2418.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
H. Lu, Y. Yang, E. M. Allister, N. Wijesekara, and M. B. Wheeler
The Identification of Potential Factors Associated with the Development of Type 2 Diabetes: A Quantitative Proteomics Approach
Mol. Cell. Proteomics, August 1, 2008; 7(8): 1434 - 1451.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
A. Pierce, R. D. Unwin, C. A. Evans, S. Griffiths, L. Carney, L. Zhang, E. Jaworska, C.-F. Lee, D. Blinco, M. J. Okoniewski, et al.
Eight-channel iTRAQ Enables Comparison of the Activity of Six Leukemogenic Tyrosine Kinases
Mol. Cell. Proteomics, May 1, 2008; 7(5): 853 - 863.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
A. J. K. Williamson, D. L. Smith, D. Blinco, R. D. Unwin, S. Pearson, C. Wilson, C. Miller, L. Lancashire, G. Lacaud, V. Kouskoff, et al.
Quantitative Proteomics Analysis Demonstrates Post-transcriptional Regulation of Embryonic Stem Cell Differentiation to Hematopoiesis
Mol. Cell. Proteomics, March 1, 2008; 7(3): 459 - 472.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
E. Spooncer, N. Brouard, S. K. Nilsson, B. Williams, M. C. Liu, R. D. Unwin, D. Blinco, E. Jaworska, P. J. Simmons, and A. D. Whetton
Developmental Fate Determination and Marker Discovery in Hematopoietic Stem Cell Biology Using Proteomic Fingerprinting
Mol. Cell. Proteomics, March 1, 2008; 7(3): 573 - 581.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
J. C. Cheng, K. M. Sakamoto, E. M. Horwitz, S. L. Karsten, L. Shoemaker, H. I. Kornblumc, and P. Malik
Report on the Workshop "New Technologies in Stem Cell Research," Society for Pediatric Research, San Francisco, California, April 29, 2006
Stem Cells, April 1, 2007; 25(4): 1070 - 1088.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. G. Janech, J. R. Raymond, and J. M. Arthur
Proteomics in renal research
Am J Physiol Renal Physiol, February 1, 2007; 292(2): F501 - F512.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
Y. Guo, Z. Fu, and J. E. Van Eyk
A Proteomic Primer for the Clinician
Proceedings of the ATS, January 1, 2007; 4(1): 9 - 17.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. D. Unwin, D. L. Smith, D. Blinco, C. L. Wilson, C. J. Miller, C. A. Evans, E. Jaworska, S. A. Baldwin, K. Barnes, A. Pierce, et al.
Quantitative proteomics reveals posttranslational control as a regulatory factor in primary hematopoietic stem cells
Blood, June 15, 2006; 107(12): 4687 - 4694.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
K. Aggarwal, L. H. Choe, and K. H. Lee
Shotgun proteomics using the iTRAQ isobaric tags
Brief Funct Genomic Proteomic, June 1, 2006; 5(2): 112 - 120.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement