HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: M400175-MCP200v1 M400175-MCP200v2 4/8/1052    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pedersen, S. K. Articles by Harry, J. L. Search for Related Content PubMed PubMed Citation Articles by Pedersen, S. K. Articles by Harry, J. L. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 4:1052-1060, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

An Immunoproteomic Approach for Identification of Clinical Biomarkers for Monitoring Disease

Application to Cystic Fibrosis^*,S

Susanne K. Pedersen^,, Andrew J. Sloane, Sindhu S. Prasad, Lucille T. Sebastian, Robyn A. Lindner, Michael Hsu, Michael Robinson^¶, Peter T. Bye^¶, Ron P. Weinberger and Jenny L. Harry

From Proteome Systems Ltd., 1/35-41 Waterloo Road, North Ryde, New South Wales 2113 and the ^¶ Royal Prince Alfred Hospital, Missenden Road, Camperdown, New South Wales 2050, Australia

Circulating antibodies can be used to probe protein arrays of body fluids, prepared by two-dimensional gel electrophoresis, for antigenic biomarker detection. However, detected proteins, particularly low abundance antigens, often remain unidentifiable due to proteome complexity and limiting sample amounts. Using a novel enrichment approach exploiting patient antibodies for isolation of antigenic biomarkers, we demonstrate how immunoproteomic strategies can accelerate biomarker discovery. Application of this approach as a means of identifying biomarkers was demonstrated for cystic fibrosis (CF) lung disease by isolation and identification of inflammatory-associated autoantigens, including myeloperoxidase and calgranulin B from sputum of subjects with CF. The approach was also exploited for isolation of proteins expressed by the Pseudomonas aeruginosa strain PA01. Capture of PA01 antigens using circulating antibodies from CF subjects implicated in vivo expression of Pseudomonas proteins. All CF subjects screened, but not controls, were immunoreactive against immunocaptured Pseudomonas proteins, representing stress (GroES and ferric iron-binding protein HitA), immunosuppressive (thioredoxin), and alginate synthetase pathway (nucleoside-diphosphate kinase) proteins, implicating their clinical relevance as biomarkers of infection.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				A. M. Trzcinska-Daneluti, D. Ly, L. Huynh, C. Jiang, C. Fladd, and D. Rotin High-content Functional Screen to Identify Proteins that Correct F508del-CFTR Function Mol. Cell. Proteomics, April 1, 2009; 8(4): 780 - 790. [Abstract] [Full Text] [PDF]

				R. D. Gray, G. MacGregor, D. Noble, M. Imrie, M. Dewar, A. C. Boyd, J. A. Innes, D. J. Porteous, and A. P. Greening Sputum Proteomics in Inflammatory and Suppurative Respiratory Diseases Am. J. Respir. Crit. Care Med., September 1, 2008; 178(5): 444 - 452. [Abstract] [Full Text] [PDF]

				S. D. Sagel, J. F. Chmiel, and M. W. Konstan Sputum Biomarkers of Inflammation in Cystic Fibrosis Lung Disease Proceedings of the ATS, August 1, 2007; 4(4): 406 - 417. [Abstract] [Full Text] [PDF]

				A. Pitarch, A. Jimenez, C. Nombela, and C. Gil Decoding Serological Response to Candida Cell Wall Immunome into Novel Diagnostic, Prognostic, and Therapeutic Candidates for Systemic Candidiasis by Proteomic and Bioinformatic Analyses Mol. Cell. Proteomics, January 1, 2006; 5(1): 79 - 96. [Abstract] [Full Text] [PDF]