HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Tables All Versions of this Article: M500049-MCP200v1 4/8/1095    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Barile, M. Articles by Knepper, M. A. Search for Related Content PubMed PubMed Citation Articles by Barile, M. Articles by Knepper, M. A. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 4:1095-1106, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Large Scale Protein Identification in Intracellular Aquaporin-2 Vesicles from Renal Inner Medullary Collecting Duct^*,S

Maria Barile^,, Trairak Pisitkun^,¶, Ming-Jiun Yu, Chung-Lin Chou, Michael J. Verbalis, Rong-Fong Shen^|| and Mark A. Knepper^,**

From the Laboratory of Kidney and Electrolyte Metabolism and ^|| Proteomics Core Facility, NHLBI, National Institutes of Health, Bethesda, Maryland 20892

Vasopressin acts on renal collecting duct cells to stimulate translocation of aquaporin-2 (AQP2)-containing membrane vesicles from throughout the cytoplasm to the apical region. The vesicles fuse with the plasma membrane to increase water permeability. To identify the intracellular membrane compartments that contain AQP2, we carried out LC-MS/MS-based proteomic analysis of immunoisolated AQP2-containing intracellular vesicles from rat inner medullary collecting duct. Immunogold electron microscopy and immunoblotting confirmed heavy AQP2 labeling of immunoisolated vesicles. Vesicle proteins were separated by SDS-PAGE followed by in-gel trypsin digestion in consecutive gel slices and identification by LC-MS/MS. Identification of Rab GTPases 4, 5, 18, and 21 (associated with early endosomes); Rab7 (late endosomes); and Rab11 and Rab25 (recycling endosomes) indicate that a substantial fraction of intracellular AQP2 is present in endosomal compartments. In addition, several endosome-associated SNARE proteins were identified including syntaxin-7, syntaxin-12, syntaxin-13, Vti1a, vesicle-associated membrane protein 2, and vesicle-associated membrane protein 3. Rab3 was not found, however, either by mass spectrometry or immunoblotting, suggesting a relative lack of AQP2 in secretory vesicles. Additionally, we identified markers of the trans-Golgi network, components of the exocyst complex, and several motor proteins including myosin 1C, non-muscle myosins IIA and IIB, myosin VI, and myosin IXB. Beyond this, identification of multiple endoplasmic reticulum-resident proteins and ribosomal proteins indicated that a substantial fraction of intracellular AQP2 is present in rough endoplasmic reticulum. These results show that AQP2-containing vesicles are heterogeneous and that intracellular AQP2 resides chiefly in endosomes, trans-Golgi network, and rough endoplasmic reticulum.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				U. Hasler, P. Nunes, R. Bouley, H. A. J. Lu, T. Matsuzaki, and D. Brown Acute Hypertonicity Alters Aquaporin-2 Trafficking and Induces a MAPK-dependent Accumulation at the Plasma Membrane of Renal Epithelial Cells J. Biol. Chem., September 26, 2008; 283(39): 26643 - 26661. [Abstract] [Full Text] [PDF]

				Z. Cao, C. Li, J. N. Higginbotham, J. L. Franklin, D. L. Tabb, R. Graves-Deal, S. Hill, K. Cheek, W. G. Jerome, L. A. Lapierre, et al. Use of Fluorescence-activated Vesicle Sorting for Isolation of Naked2-associated, Basolaterally Targeted Exocytic Vesicles for Proteomics Analysis Mol. Cell. Proteomics, September 1, 2008; 7(9): 1651 - 1667. [Abstract] [Full Text] [PDF]

				M.-J. Yu, T. Pisitkun, G. Wang, J. F. Aranda, P. A. Gonzales, D. Tchapyjnikov, R.-F. Shen, M. A. Alonso, and M. A. Knepper Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct Am J Physiol Cell Physiol, September 1, 2008; 295(3): C661 - C678. [Abstract] [Full Text] [PDF]

				T. Schallus, C. Jaeckh, K. Feher, A. S. Palma, Y. Liu, J. C. Simpson, M. Mackeen, G. Stier, T. J. Gibson, T. Feizi, et al. Malectin: A Novel Carbohydrate-binding Protein of the Endoplasmic Reticulum and a Candidate Player in the Early Steps of Protein N-Glycosylation Mol. Biol. Cell, August 1, 2008; 19(8): 3404 - 3414. [Abstract] [Full Text] [PDF]

				Y.-J. Lee, H.-J. Choi, J.-S. Lim, J.-H. Earm, B.-H. Lee, I.-S. Kim, J. Frokiaer, S. Nielsen, and T.-H. Kwon A novel method of ligand peptidomics to identify peptide ligands binding to AQP2-expressing plasma membranes and intracellular vesicles of rat kidney Am J Physiol Renal Physiol, July 1, 2008; 295(1): F300 - F309. [Abstract] [Full Text] [PDF]

				G. Procino, C. Barbieri, G. Tamma, L. De Benedictis, J. E. Pessin, M. Svelto, and G. Valenti AQP2 exocytosis in the renal collecting duct - involvement of SNARE isoforms and the regulatory role of Munc18b J. Cell Sci., June 15, 2008; 121(12): 2097 - 2106. [Abstract] [Full Text] [PDF]

				P. Uawithya, T. Pisitkun, B. E. Ruttenberg, and M. A. Knepper Transcriptional profiling of native inner medullary collecting duct cells from rat kidney Physiol Genomics, January 17, 2008; 32(2): 229 - 253. [Abstract] [Full Text] [PDF]

				T. Pisitkun, J. D. Hoffert, M.-J. Yu, and M. A. Knepper Tandem Mass Spectrometry in Physiology Physiology, December 1, 2007; 22(6): 390 - 400. [Abstract] [Full Text] [PDF]

				M. Goel, W. G. Sinkins, C.-D. Zuo, U. Hopfer, and W. P. Schilling Vasopressin-induced membrane trafficking of TRPC3 and AQP2 channels in cells of the rat renal collecting duct Am J Physiol Renal Physiol, November 1, 2007; 293(5): F1476 - F1488. [Abstract] [Full Text] [PDF]

				R. A. Fenton and M. A. Knepper Mouse Models and the Urinary Concentrating Mechanism in the New Millennium Physiol Rev, October 1, 2007; 87(4): 1083 - 1112. [Abstract] [Full Text] [PDF]

				A. Oztan, M. Silvis, O. A. Weisz, N. A. Bradbury, S.-C. Hsu, J. R. Goldenring, C. Yeaman, and G. Apodaca Exocyst Requirement for Endocytic Traffic Directed Toward the Apical and Basolateral Poles of Polarized MDCK Cells Mol. Biol. Cell, October 1, 2007; 18(10): 3978 - 3992. [Abstract] [Full Text] [PDF]

				A. Vossenkamper, P. I. Nedvetsky, B. Wiesner, J. Furkert, W. Rosenthal, and E. Klussmann Microtubules are needed for the perinuclear positioning of aquaporin-2 after its endocytic retrieval in renal principal cells Am J Physiol Cell Physiol, September 1, 2007; 293(3): C1129 - C1138. [Abstract] [Full Text] [PDF]

				L. A. Lapierre, K. M. Avant, C. M. Caldwell, A.-J. L. Ham, S. Hill, J. A. Williams, A. J. Smolka, and J. R. Goldenring Characterization of immunoisolated human gastric parietal cells tubulovesicles: identification of regulators of apical recycling Am J Physiol Gastrointest Liver Physiol, May 1, 2007; 292(5): G1249 - G1262. [Abstract] [Full Text] [PDF]

				J. H. Robben, M. Sze, N. V. Knoers, P. Eggert, P. Deen, and D. Muller Relief of Nocturnal Enuresis by Desmopressin Is Kidney and Vasopressin Type 2 Receptor Independent J. Am. Soc. Nephrol., May 1, 2007; 18(5): 1534 - 1539. [Abstract] [Full Text] [PDF]

				J. D. Hoffert, J. Nielsen, M.-J. Yu, T. Pisitkun, S. M. Schleicher, S. Nielsen, and M. A. Knepper Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct Am J Physiol Renal Physiol, February 1, 2007; 292(2): F691 - F700. [Abstract] [Full Text] [PDF]

				S. M. Malakauskas, H. Quan, T. A. Fields, S. J. McCall, M.-J. Yu, W. M. Kourany, C. W. Frey, and T. H. Le Aminoaciduria and altered renal expression of luminal amino acid transporters in mice lacking novel gene collectrin Am J Physiol Renal Physiol, February 1, 2007; 292(2): F533 - F544. [Abstract] [Full Text] [PDF]

				M. G. Janech, J. R. Raymond, and J. M. Arthur Proteomics in renal research Am J Physiol Renal Physiol, February 1, 2007; 292(2): F501 - F512. [Abstract] [Full Text] [PDF]

				M.-J. Yu, T. Pisitkun, G. Wang, R.-F. Shen, and M. A. Knepper LC-MS/MS Analysis of Apical and Basolateral Plasma Membranes of Rat Renal Collecting Duct Cells Mol. Cell. Proteomics, November 1, 2006; 5(11): 2131 - 2145. [Abstract] [Full Text] [PDF]

				T. Pisitkun, J. Bieniek, D. Tchapyjnikov, G. Wang, W. W. Wu, R.-F. Shen, and M. A. Knepper High-throughput identification of IMCD proteins using LC-MS/MS Physiol Genomics, April 13, 2006; 25(2): 263 - 276. [Abstract] [Full Text] [PDF]

				P. A. Ortiz cAMP increases surface expression of NKCC2 in rat thick ascending limbs: role of VAMP Am J Physiol Renal Physiol, March 1, 2006; 290(3): F608 - F616. [Abstract] [Full Text] [PDF]

				G. Valenti, G. Procino, G. Tamma, M. Carmosino, and M. Svelto Minireview: Aquaporin 2 Trafficking Endocrinology, December 1, 2005; 146(12): 5063 - 5070. [Abstract] [Full Text] [PDF]