MCP Waters-The Science of What's Possible
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M500041-MCP200 on June 12, 2005.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Data
Right arrow All Versions of this Article:
M500041-MCP200v1
4/9/1251    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Glossary
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, J.
Right arrow Articles by Thomassen, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, J.
Right arrow Articles by Thomassen, M. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 4:1251-1264, 2005.
© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Differential Proteomic Analysis of Bronchoalveolar Lavage Fluid in Asthmatics following Segmental Antigen Challenge*,S

Jiang Wu{ddagger}, Michiko Kobayashi§, Eric A. Sousa{ddagger}, Wei Liu{ddagger}, Jie Cai§, Samuel J. Goldman§, Andrew J. Dorner{ddagger}, Steven J. Projan§, Mani S. Kavuru, Yongchang Qiu{ddagger},|| and Mary Jane Thomassen

From {ddagger} Biological Technologies and § Inflammation Research, Wyeth, Cambridge, Massachusetts 02140 and the Cleveland Clinic Foundation, Cleveland, Ohio 44195

Allergic asthma is characterized by persistent airway inflammation and remodeling. Bronchoalveolar lavage conducted with fiberoptic bronchoscopy has been widely used for investigating the pathogenesis of asthma and other lung disorders. Identification of proteins in the bronchoalveolar lavage fluid (BALF) and their expression changes at different stages of asthma could provide further insights into the complex molecular mechanisms involved in this disease. In this report, we describe the first comprehensive differential proteomic analysis of BALF from both asthmatic patients and healthy subjects before and 24 h after segmental allergen challenge. Our proteomic analysis involves affinity depletion of six abundant BALF proteins, SDS-PAGE fractionation, protein in-gel digestion, and subsequent nano-LC-MS/MS analysis in conjunction with database searching for protein identification and semiquantitation. More than 1,500 distinct proteins were identified of which about 10% displayed significant up-regulation specific to the asthmatic patients after segmental allergen challenge. The differentially expressed proteins represent a wide spectrum of functional classes such as chemokines, cytokines, proteases, complement factors, acute phase proteins, monocyte-specific granule proteins, and local matrix proteins, etc. The majority of these protein expression changes are closely associated with many aspects of the pathophysiology of asthma, including inflammation, eosinophilia, airway remodeling, tissue damage and repair, mucus production, and plasma infiltration. Importantly a large portion of these proteins and their expression changes were identified for the first time from BALF, thus providing new insights for finding novel pathological mediators and biomarkers of asthma.

|| To whom correspondence should be addressed: Biological Technologies, Wyeth Research, 200 Cambridge Park Dr., Cambridge, MA 02140. Tel.: 617-665-8139; Fax: 617-665-8435; E-mail: ycqiu{at}wyeth.com


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
C. L. Ventura, R. Higdon, L. Hohmann, D. Martin, E. Kolker, H. D. Liggitt, S. J. Skerrett, and C. E. Rubens
Staphylococcus aureus Elicits Marked Alterations in the Airway Proteome during Early Pneumonia
Infect. Immun., December 1, 2008; 76(12): 5862 - 5872.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. G. Therien, V. Bernier, S. Weicker, P. Tawa, J.-P. Falgueyret, M.-C. Mathieu, J. Honsberger, V. Pomerleau, A. Robichaud, R. Stocco, et al.
Adenovirus IL-13-Induced Airway Disease in Mice: A Corticosteroid-Resistant Model of Severe Asthma
Am. J. Respir. Cell Mol. Biol., July 1, 2008; 39(1): 26 - 35.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
F. A. Barnes, L. Bingle, and C. D. Bingle
Pulmonary Genomics, Proteomics, and PLUNCs
Am. J. Respir. Cell Mol. Biol., April 1, 2008; 38(4): 377 - 379.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
B. J. McMorran, S. A. Ouvry Patat, J. B. Carlin, K. Grimwood, A. Jones, D. S. Armstrong, J. C. Galati, P. J. Cooper, C. A. Byrnes, P. W. Francis, et al.
Novel Neutrophil-Derived Proteins in Bronchoalveolar Lavage Fluid Indicate an Exaggerated Inflammatory Response in Pediatric Cystic Fibrosis Patients
Clin. Chem., October 1, 2007; 53(10): 1782 - 1791.
[Abstract] [Full Text] [PDF]

Home page
 ThoraxHome page
Poster presentations
Thorax, December 1, 2006; 61(suppl_2): ii57 - ii133.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. Plymoth, Z. Yang, C.-G. Lofdahl, A. Ekberg-Jansson, M. Dahlback, T. E. Fehniger, G. Marko-Varga, and W. S. Hancock
Rapid Proteome Analysis of Bronchoalveolar Lavage Samples of Lifelong Smokers and Never-Smokers by Micro-Scale Liquid Chromatography and Mass Spectrometry
Clin. Chem., April 1, 2006; 52(4): 671 - 679.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2005 by the American Society for Biochemistry and Molecular Biology.