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Originally published In Press as doi:10.1074/mcp.R500014-MCP200 on October 28, 2005.
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Molecular & Cellular Proteomics 5:2-13, 2006.
© 2006 by The American Society for Biochemistry and Molecular Biology, Inc.

Review

Integrating Cytomics and Proteomics*

Tytus Bernas{ddagger}, Gérald Grégori§, Eli K. Asem and J. Paul Robinson{ddagger},||

From the {ddagger} Cytometry Laboratories and School of Veterinary Medicine, Purdue University, West Lafayette, Indiana 47907-2064 and § Laboratoire de Microbiologie, Géochimie et Ecologíe Marines, CNRS UMR 6117, 163 Avenue de Luminy, Case 901, Batîment TPR1, 13288 Marseille cedex 9, France

Systems biology along with what is now classified as cytomics provides an excellent opportunity for cytometry to become integrated into studies where identification of functional proteins in complex cellular mixtures is desired. The combination of cell sorting with rapid protein-profiling platforms offers an automated and rapid technique for greater clarity, accuracy, and efficiency in identification of protein expression differences in mixed cell populations. The integration of cell sorting to purify cell populations opens up a new area for proteomic analysis. This article outlines an approach in which well defined cell analysis and separation tools are integrated into the proteomic programs within a core laboratory. In addition we introduce the concepts of flow cytometry sorting to demonstrate the importance of being able to use flow cytometry as a cell separation technology to identify and collect purified cell populations. Data demonstrating the speed and versatility of this combination of flow cytometry-based cell separation and protein separation and subsequent analysis, examples of protein maps from purified sorted cells, and an analysis of the overall procedure will be shown. It is clear that the power of cell sorting to separate heterogeneous populations of cells using specific phenotypic characteristics increases the power of rapid automated protein separation technologies.

|| To whom correspondence should be addressed: Cytometry Laboratories, Hansen Life Sciences Research Bldg., Rm. B050, Purdue University, 201 South University St., West Lafayette, IN 47907-2064. E-mail: jpr{at}flowcyt.cyto.purdue.edu


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