Advertisement
MCP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M600176-MCP200 on June 7, 2006.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M600176-MCP200v1
5/10/1957    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Glossary
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Drake, R. R.
Right arrow Articles by Semmes, O. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Drake, R. R.
Right arrow Articles by Semmes, O. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 5:1957-1967, 2006.
© 2006 by The American Society for Biochemistry and Molecular Biology, Inc.

Methodologies

Lectin Capture Strategies Combined with Mass Spectrometry for the Discovery of Serum Glycoprotein Biomarkers*

Richard R. Drake{ddagger}, E. Ellen Schwegler{ddagger}, Gunjan Malik{ddagger}, Jose Diaz{ddagger}, Timothy Block§, Anand Mehta§ and O. John Semmes{ddagger},¶

From the {ddagger} Center for Biomedical Proteomics, Virginia Prostate Center, and Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, Virginia 23507 and § Drexel Institute of Biotechnology and Virology Research, Doylestown, Pennsylvania 18901

The application of mass spectrometry to identify disease biomarkers in clinical fluids like serum using high throughput protein expression profiling continues to evolve as technology development, clinical study design, and bioinformatics improve. Previous protein expression profiling studies have offered needed insight into issues of technical reproducibility, instrument calibration, sample preparation, study design, and supervised bioinformatic data analysis. In this overview, new strategies to increase the utility of protein expression profiling for clinical biomarker assay development are discussed with an emphasis on utilizing differential lectin-based glycoprotein capture and targeted immunoassays. The carbohydrate binding specificities of different lectins offer a biological affinity approach that complements existing mass spectrometer capabilities and retains automated throughput options. Specific examples using serum samples from prostate cancer and hepatocellular carcinoma subjects are provided along with suggested experimental strategies for integration of lectin-based methods into clinical fluid expression profiling strategies. Our example workflow incorporates the necessity of early validation in biomarker discovery using an immunoaffinity-based targeted analytical approach that integrates well with upstream discovery technologies.

To whom correspondence should be addressed: Dept. of Microbiology and Molecular Cell Biology, Lewis Hall 3144, Eastern Virginia Medical School, Norfolk, VA 23507. Tel.: 757-446-5904; Fax: 757-446-5766; E-mail: semmesoj{at}evms.edu


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
M. Wang, R. E. Long, M. A. Comunale, O. Junaidi, J. Marrero, A. M. Di Bisceglie, T. M. Block, and A. S. Mehta
Novel Fucosylated Biomarkers for the Early Detection of Hepatocellular Carcinoma
Cancer Epidemiol. Biomarkers Prev., June 1, 2009; 18(6): 1914 - 1921.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
W. Jia, Z. Lu, Y. Fu, H.-P. Wang, L.-H. Wang, H. Chi, Z.-F. Yuan, Z.-B. Zheng, L.-N. Song, H.-H. Han, et al.
A Strategy for Precise and Large Scale Identification of Core Fucosylated Glycoproteins
Mol. Cell. Proteomics, May 1, 2009; 8(5): 913 - 923.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
A. Kuno, Y. Kato, A. Matsuda, M. K. Kaneko, H. Ito, K. Amano, Y. Chiba, H. Narimatsu, and J. Hirabayashi
Focused Differential Glycan Analysis with the Platform Antibody-assisted Lectin Profiling for Glycan-related Biomarker Verification
Mol. Cell. Proteomics, January 1, 2009; 8(1): 99 - 108.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. S. Mehta, R. E. Long, M. A. Comunale, M. Wang, L. Rodemich, J. Krakover, R. Philip, J. A. Marrero, R. A. Dwek, and T. M. Block
Increased Levels of Galactose-Deficient Anti-Gal Immunoglobulin G in the Sera of Hepatitis C Virus-Infected Individuals with Fibrosis and Cirrhosis
J. Virol., February 1, 2008; 82(3): 1259 - 1270.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
L. Harris, H. Fritsche, R. Mennel, L. Norton, P. Ravdin, S. Taube, M. R. Somerfield, D. F. Hayes, and R. C. Bast Jr
American Society of Clinical Oncology 2007 Update of Recommendations for the Use of Tumor Markers in Breast Cancer
J. Clin. Oncol., November 20, 2007; 25(33): 5287 - 5312.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
B. Gangadharan, R. Antrobus, R. A. Dwek, and N. Zitzmann
Novel Serum Biomarker Candidates for Liver Fibrosis in Hepatitis C Patients
Clin. Chem., October 1, 2007; 53(10): 1792 - 1799.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement