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Originally published In Press as doi:10.1074/mcp.T600039-MCP200 on July 19, 2006.
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Molecular & Cellular Proteomics 5:2167-2174, 2006.
© 2006 by The American Society for Biochemistry and Molecular Biology, Inc.

Technology

Evaluation of Multiprotein Immunoaffinity Subtraction for Plasma Proteomics and Candidate Biomarker Discovery Using Mass Spectrometry*,S

Tao Liu, Wei-Jun Qian, Heather M. Mottaz, Marina A. Gritsenko, Angela D. Norbeck, Ronald J. Moore, Samuel O. Purvine, David G. Camp, II and Richard D. Smith{ddagger}

From the Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington 99352

Strategies for removal of high abundance proteins have been increasingly utilized in proteomic studies of serum/plasma and other body fluids to enhance the detection of low abundance proteins and achieve broader proteome coverage; however, both the reproducibility and specificity of the high abundance protein depletion process still represent common concerns. Here we report a detailed evaluation of immunoaffinity subtraction performed applying the ProteomeLab IgY-12 system that is commonly used in human serum/plasma proteome characterization in combination with high resolution LC-MS/MS. Plasma samples were repeatedly processed using this approach, and the resulting flow-through fractions and bound fractions were individually analyzed for comparison. The removal of target proteins by the immunoaffinity subtraction system and the overall process was highly reproducible. Non-target proteins, including one spiked protein standard (rabbit glyceraldehyde-3-phosphate dehydrogenase), were also observed to bind to the column at different levels but also in a reproducible manner. The results suggest that multiprotein immunoaffinity subtraction systems can be readily integrated into quantitative strategies to enhance detection of low abundance proteins in biomarker discovery studies.

{ddagger} To whom correspondence should be addressed: Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, P. O. Box 999, MSIN: K8-98, Richland, WA 99352. E-mail: rds{at}pnl.gov


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Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.