|
|
||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Molecular & Cellular Proteomics 5:256-264, 2006.
© 2006 by The American Society for Biochemistry and Molecular Biology, Inc.
,
,¶From the Department of Biological Sciences, National University of Singapore, 21 Lower Kent Ridge Road, Singapore 119077, Singapore
The Singapore grouper iridovirus (SGIV) genome consists of a double-stranded circular DNA of 140,131 base pairs with 162 predicted open reading frames. Our earlier study using peptide mass fingerprints generated from MALDI-TOF MS led to the identification of 26 viral proteins. The present investigation aimed to achieve a more comprehensive and precise identification of the SGIV viral proteome by two workflows: one-dimensional gel electrophoresis (1-DE) separation followed by protein identification by MALDI-TOF/TOF MS/MS (1-DE-MALDI workflow) and shotgun proteomics in which the whole virus was digested by trypsin and the resulting peptides were separated by nano-LC and analyzed by MALDI-TOF/TOF MS/MS (LC-MALDI workflow). In total, 44 viral proteins were identified, 25 of which were reported for the first time. Fourteen proteins were uniquely identified by the 1-DE-MALDI workflow, whereas another 10 proteins were only identified by the LC-MALDI workflow with 20 proteins found by both approaches. Moreover 13 proteins were found to have acetylated N termini. Twenty-three proteins identified contain predicted transmembrane domains, accounting for 52.3% of the total proteins identified. RT-PCR confirmed the transcription products of all the identified viral proteins. A large number of proteins identified by both the 1-DE-MALDI and the LC-MALDI workflows from this study have significantly enhanced the coverage of the SGIV proteome. The SGIV proteome is at present the only iridoviral proteome that has been extensively characterized. Our results should provide further insights into the biology of SGIV and other iridoviruses.
![]()
CiteULike
Complore
Connotea
Del.icio.us
Digg
Reddit
Technorati What's this?
This article has been cited by other articles:
![]() |
F. Wang, X. Bi, L. M. Chen, and C.-L. Hew ORF018R, a highly abundant virion protein from Singapore grouper iridovirus, is involved in serine/threonine phosphorylation and virion assembly J. Gen. Virol., May 1, 2008; 89(5): 1169 - 1178. [Abstract] [Full Text] [PDF] |
||||
![]() |
Z. Li, Q. Lin, J. Chen, J. L. Wu, T. K. Lim, S. S. Loh, X. Tang, and C.-L. Hew Shotgun Identification of the Structural Proteome of Shrimp White Spot Syndrome Virus and iTRAQ Differentiation of Envelope and Nucleocapsid Subproteomes Mol. Cell. Proteomics, September 1, 2007; 6(9): 1609 - 1620. [Abstract] [Full Text] [PDF] |
||||
![]() |
D. A. N. Mustafa, P. C. Burgers, L. J. Dekker, H. Charif, M. K. Titulaer, P. A. E. S. Smitt, T. M. Luider, and J. M. Kros Identification of Glioma Neovascularization-related Proteins by Using MALDI-FTMS and Nano-LC Fractionation to Microdissected Tumor Vessels Mol. Cell. Proteomics, July 1, 2007; 6(7): 1147 - 1157. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. L. Maxwell and L. Frappier Viral Proteomics Microbiol. Mol. Biol. Rev., June 1, 2007; 71(2): 398 - 411. [Abstract] [Full Text] [PDF] |
||||
![]() |
L. M. Chen, F. Wang, W. Song, and C. L. Hew Temporal and differential gene expression of Singapore grouper iridovirus. J. Gen. Virol., October 1, 2006; 87(Pt 10): 2907 - 2915. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Journal of Lipid Research | ASBMB Today |