Advertisement
MCP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Originally published In Press as doi:10.1074/mcp.M500331-MCP200 on December 6, 2005.
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
M500331-MCP200v1
5/4/573    most recent
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Glossary
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Anderson, L.
Right arrow Articles by Hunter, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Anderson, L.
Right arrow Articles by Hunter, C. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Molecular & Cellular Proteomics 5:573-588, 2006.
© 2006 by The American Society for Biochemistry and Molecular Biology, Inc.

Research

Quantitative Mass Spectrometric Multiple Reaction Monitoring Assays for Major Plasma Proteins*

Leigh Anderson{ddagger},§ and Christie L. Hunter

From the {ddagger} The Plasma Proteome Institute, Washington, D. C. 20009-3450 and Applied Biosystems, Foster City, California 94404

Quantitative LC-MS/MS assays were designed for tryptic peptides representing 53 high and medium abundance proteins in human plasma using a multiplexed multiple reaction monitoring (MRM) approach. Of these, 47 produced acceptable quantitative data, demonstrating within-run coefficients of variation (CVs) (n = 10) of 2–22% (78% of assays had CV <10%). A number of peptides gave CVs in the range 2–7% in five experiments (10 replicate runs each) continuously measuring 137 MRMs, demonstrating the precision achievable in complex digests. Depletion of six high abundance proteins by immunosubtraction significantly improved CVs compared with whole plasma, but analytes could be detected in both sample types. Replicate digest and depletion/digest runs yielded correlation coefficients (R2) of 0.995 and 0.989, respectively. Absolute analyte specificity for each peptide was demonstrated using MRM-triggered MS/MS scans. Reliable detection of L-selectin (measured at 0.67 µg/ml) indicates that proteins down to the µg/ml level can be quantitated in plasma with minimal sample preparation, yielding a dynamic range of 4.5 orders of magnitude in a single experiment. Peptide MRM measurements in plasma digests thus provide a rapid and specific assay platform for biomarker validation, one that can be extended to lower abundance proteins by enrichment of specific target peptides (stable isotope standards and capture by anti-peptide antibodies (SISCAPA)).

§ To whom correspondence should be addressed: The Plasma Proteome Inst., P. O. Box 53450, Washington, D. C. 20009-3450. Tel.: 301-728-1451; Fax: 202-234-9175; E-mail: leighanderson{at}plasmaproteome.org


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Clin. Chem.Home page
N. G. Anderson
Adventures in Clinical Chemistry and Proteomics: A Personal Account
Clin. Chem., February 1, 2010; 56(2): 154 - 160.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
P. M. Drake, W. Cho, B. Li, A. Prakobphol, E. Johansen, N. L. Anderson, F. E. Regnier, B. W. Gibson, and S. J. Fisher
Sweetening the Pot: Adding Glycosylation to the Biomarker Discovery Equation
Clin. Chem., February 1, 2010; 56(2): 223 - 236.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. Makawita and E. P. Diamandis
The Bottleneck in the Cancer Biomarker Pipeline and Protein Quantification through Mass Spectrometry-Based Approaches: Current Strategies for Candidate Verification
Clin. Chem., February 1, 2010; 56(2): 212 - 222.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. R. Whiteaker, L. Zhao, L. Anderson, and A. G. Paulovich
An Automated and Multiplexed Method for High Throughput Peptide Immunoaffinity Enrichment and Multiple Reaction Monitoring Mass Spectrometry-based Quantification of Protein Biomarkers
Mol. Cell. Proteomics, January 1, 2010; 9(1): 184 - 196.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P.-Y. Liao, Y. S. Choi, and K. H. Lee
FSscan: a mechanism-based program to identify +1 ribosomal frameshift hotspots
Nucleic Acids Res., November 1, 2009; 37(21): 7302 - 7311.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. Sherman, M. J. McKay, K. Ashman, and M. P. Molloy
Unique Ion Signature Mass Spectrometry, a Deterministic Method to Assign Peptide Identity
Mol. Cell. Proteomics, September 1, 2009; 8(9): 2051 - 2062.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
M. A. Kuzyk, D. Smith, J. Yang, T. J. Cross, A. M. Jackson, D. B. Hardie, N. L. Anderson, and C. H. Borchers
Multiple Reaction Monitoring-based, Multiplexed, Absolute Quantitation of 45 Proteins in Human Plasma
Mol. Cell. Proteomics, August 1, 2009; 8(8): 1860 - 1877.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. C. Seegmiller, D. R. Barnidge, B. E. Burns, T. S. Larson, J. C. Lieske, and R. Kumar
Quantification of Urinary Albumin by Using Protein Cleavage and LC-MS/MS
Clin. Chem., June 1, 2009; 55(6): 1100 - 1107.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
E. Kuhn, T. Addona, H. Keshishian, M. Burgess, D.R. Mani, R. T. Lee, M. S. Sabatine, R. E. Gerszten, and S. A. Carr
Developing Multiplexed Assays for Troponin I and Interleukin-33 in Plasma by Peptide Immunoaffinity Enrichment and Targeted Mass Spectrometry
Clin. Chem., June 1, 2009; 55(6): 1108 - 1117.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
N. L. Anderson, N. G. Anderson, T. W. Pearson, C. H. Borchers, A. G. Paulovich, S. D. Patterson, M. Gillette, R. Aebersold, and S. A. Carr
A Human Proteome Detection and Quantitation Project
Mol. Cell. Proteomics, May 1, 2009; 8(5): 883 - 886.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
N. L. Anderson, A. Jackson, D. Smith, D. Hardie, C. Borchers, and T. W. Pearson
SISCAPA Peptide Enrichment on Magnetic Beads Using an In-line Bead Trap Device
Mol. Cell. Proteomics, May 1, 2009; 8(5): 995 - 1005.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
T. Fortin, A. Salvador, J. P. Charrier, C. Lenz, X. Lacoux, A. Morla, G. Choquet-Kastylevsky, and J. Lemoine
Clinical Quantitation of Prostate-specific Antigen Biomarker in the Low Nanogram/Milliliter Range by Conventional Bore Liquid Chromatography-Tandem Mass Spectrometry (Multiple Reaction Monitoring) Coupling and Correlation with ELISA Tests
Mol. Cell. Proteomics, May 1, 2009; 8(5): 1006 - 1015.
[Abstract] [Full Text] [PDF]

Home page
 Proc Am Thorac SocHome page
S. Ocak, P. Chaurand, and P. P. Massion
Mass Spectrometry-based Proteomic Profiling of Lung Cancer
Proceedings of the ATS, April 15, 2009; 6(2): 159 - 170.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
Y. Gao, R. D. Holland, and L.-R. Yu
Quantitative proteomics for drug toxicity
Briefings in Functional Genomics, April 7, 2009; (2009) elp006v1.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. A. Mead, L. Bianco, V. Ottone, C. Barton, R. G. Kay, K. S. Lilley, N. J. Bond, and C. Bessant
MRMaid, the Web-based Tool for Designing Multiple Reaction Monitoring (MRM) Transitions
Mol. Cell. Proteomics, April 1, 2009; 8(4): 696 - 705.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
A. K. Yocum and A. M. Chinnaiyan
Current affairs in quantitative targeted proteomics: multiple reaction monitoring-mass spectrometry
Briefings in Functional Genomics, March 11, 2009; (2009) eln056v1.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
X. Ye, J. Blonder, and T. D. Veenstra
Targeted proteomics for validation of biomarkers in clinical samples
Briefings in Functional Genomics, March 1, 2009; 8(2): 126 - 135.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
A. Kuno, Y. Kato, A. Matsuda, M. K. Kaneko, H. Ito, K. Amano, Y. Chiba, H. Narimatsu, and J. Hirabayashi
Focused Differential Glycan Analysis with the Platform Antibody-assisted Lectin Profiling for Glycan-related Biomarker Verification
Mol. Cell. Proteomics, January 1, 2009; 8(1): 99 - 108.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. deWilde, K. Sadilkova, M. Sadilek, V. Vasta, and S. H. Hahn
Tryptic Peptide Analysis of Ceruloplasmin in Dried Blood Spots Using Liquid Chromatography-Tandem Mass Spectrometry: Application to Newborn Screening
Clin. Chem., December 1, 2008; 54(12): 1961 - 1968.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
S. A. Carr and L. Anderson
Protein Quantitation through Targeted Mass Spectrometry: The Way Out of Biomarker Purgatory?
Clin. Chem., November 1, 2008; 54(11): 1749 - 1752.
[Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
D. B. Martin, T. Holzman, D. May, A. Peterson, A. Eastham, J. Eng, and M. McIntosh
MRMer, an Interactive Open Source and Cross-platform System for Data Extraction and Visualization of Multiple Reaction Monitoring Experiments
Mol. Cell. Proteomics, November 1, 2008; 7(11): 2270 - 2278.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
G. R. Nicol, M. Han, J. Kim, C. E. Birse, E. Brand, A. Nguyen, M. Mesri, W. FitzHugh, P. Kaminker, P. A. Moore, et al.
Use of an Immunoaffinity-Mass Spectrometry-based Approach for the Quantification of Protein Biomarkers from Serum Samples of Lung Cancer Patients
Mol. Cell. Proteomics, October 1, 2008; 7(10): 1974 - 1982.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
W.-J. Qian, D. T. Kaleta, B. O. Petritis, H. Jiang, T. Liu, X. Zhang, H. M. Mottaz, S. M. Varnum, D. G. Camp II, L. Huang, et al.
Enhanced Detection of Low Abundance Human Plasma Proteins Using a Tandem IgY12-SuperMix Immunoaffinity Separation Strategy
Mol. Cell. Proteomics, October 1, 2008; 7(10): 1963 - 1973.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. M. Koomen, E. B. Haura, G. Bepler, R. Sutphen, E. R. Remily-Wood, K. Benson, M. Hussein, L. A. Hazlehurst, T. J. Yeatman, L. T. Hildreth, et al.
Proteomic Contributions to Personalized Cancer Care
Mol. Cell. Proteomics, October 1, 2008; 7(10): 1780 - 1794.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
U. Kruse, M. Bantscheff, G. Drewes, and C. Hopf
Chemical and Pathway Proteomics: Powerful Tools for Oncology Drug Discovery and Personalized Health Care
Mol. Cell. Proteomics, October 1, 2008; 7(10): 1887 - 1901.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
G. E. Merrihew, C. Davis, B. Ewing, G. Williams, L. Kall, B. E. Frewen, W. S. Noble, P. Green, J. H. Thomas, and M. J. MacCoss
Use of shotgun proteomics for the identification, confirmation, and correction of C. elegans gene annotations
Genome Res., October 1, 2008; 18(10): 1660 - 1669.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
G. L. Hortin, D. Sviridov, and N. L. Anderson
High-Abundance Polypeptides of the Human Plasma Proteome Comprising the Top 4 Logs of Polypeptide Abundance
Clin. Chem., October 1, 2008; 54(10): 1608 - 1616.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
B. Han and R. E. Higgs
Proteomics: from hypothesis to quantitative assay on a single platform. Guidelines for developing MRM assays using ion trap mass spectrometers
Briefings in Functional Genomics, September 1, 2008; 7(5): 340 - 354.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
S. Loret, G. Guay, and R. Lippe
Comprehensive Characterization of Extracellular Herpes Simplex Virus Type 1 Virions
J. Virol., September 1, 2008; 82(17): 8605 - 8618.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
V. Lange, J. A. Malmstrom, J. Didion, N. L. King, B. P. Johansson, J. Schafer, J. Rameseder, C.-H. Wong, E. W. Deutsch, M.-Y. Brusniak, et al.
Targeted Quantitative Analysis of Streptococcus pyogenes Virulence Factors by Multiple Reaction Monitoring
Mol. Cell. Proteomics, August 1, 2008; 7(8): 1489 - 1500.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
M. B. Lucitt, T. S. Price, A. Pizarro, W. Wu, A. K. Yocum, C. Seiler, M. A. Pack, I. A. Blair, G. A. FitzGerald, and T. Grosser
Analysis of the Zebrafish Proteome during Embryonic Development
Mol. Cell. Proteomics, May 1, 2008; 7(5): 981 - 994.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
A. K. Yocum, T. E. Gratsch, N. Leff, J. R. Strahler, C. L. Hunter, A. K. Walker, G. Michailidis, G. S. Omenn, K. S. O'Shea, and P. C. Andrews
Coupled Global and Targeted Proteomics of Human Embryonic Stem Cells during Induced Differentiation
Mol. Cell. Proteomics, April 1, 2008; 7(4): 750 - 767.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. Fujii, G. Cuvelier, K. She, S. Aslanian, H. Shimizu, A. Kariminia, M. Krailo, Z. Chen, R. McMaster, A. Bergman, et al.
Biomarkers in newly diagnosed pediatric-extensive chronic graft-versus-host disease: a report from the Children's Oncology Group
Blood, March 15, 2008; 111(6): 3276 - 3285.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. Villanueva, A. Nazarian, K. Lawlor, S. S. Yi, R. J. Robbins, and P. Tempst
A Sequence-specific Exopeptidase Activity Test (SSEAT) for "Functional" Biomarker Discovery
Mol. Cell. Proteomics, March 1, 2008; 7(3): 509 - 518.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
J. N. McGuire, J. Overgaard, and F. Pociot
Mass spectrometry is only one piece of the puzzle in clinical proteomics
Briefings in Functional Genomics, February 28, 2008; (2008) eln005v1.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
C. Jorgensen and R. Linding
Directional and quantitative phosphorylation networks
Briefings in Functional Genomics, February 12, 2008; (2008) eln001v1.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
B. A. Merrick
The plasma proteome, adductome and idiosyncratic toxicity in toxicoproteomics research
Briefings in Functional Genomics, February 12, 2008; (2008) eln004v1.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
V. Brun, A. Dupuis, A. Adrait, M. Marcellin, D. Thomas, M. Court, F. Vandenesch, and J. Garin
Isotope-labeled Protein Standards: Toward Absolute Quantitative Proteomics
Mol. Cell. Proteomics, December 1, 2007; 6(12): 2139 - 2149.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
H. Keshishian, T. Addona, M. Burgess, E. Kuhn, and S. A. Carr
Quantitative, Multiplexed Assays for Low Abundance Proteins in Plasma by Targeted Mass Spectrometry and Stable Isotope Dilution
Mol. Cell. Proteomics, December 1, 2007; 6(12): 2212 - 2229.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
V. Kulasingam and E. P. Diamandis
Proteomics Analysis of Conditioned Media from Three Breast Cancer Cell Lines: A Mine for Biomarkers and Therapeutic Targets
Mol. Cell. Proteomics, November 1, 2007; 6(11): 1997 - 2011.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
H. Zhang and D. W. Chan
Cancer Biomarker Discovery in Plasma Using a Tissue-targeted Proteomic Approach
Cancer Epidemiol. Biomarkers Prev., October 1, 2007; 16(10): 1915 - 1917.
[Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
J. Stahl-Zeng, V. Lange, R. Ossola, K. Eckhardt, W. Krek, R. Aebersold, and B. Domon
High Sensitivity Detection of Plasma Proteins by Multiple Reaction Monitoring of N-Glycosites
Mol. Cell. Proteomics, October 1, 2007; 6(10): 1809 - 1817.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
P. Picotti, R. Aebersold, and B. Domon
The Implications of Proteolytic Background for Shotgun Proteomics
Mol. Cell. Proteomics, September 1, 2007; 6(9): 1589 - 1598.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
P. R. Cutillas and B. Vanhaesebroeck
Quantitative Profile of Five Murine Core Proteomes Using Label-free Functional Proteomics
Mol. Cell. Proteomics, September 1, 2007; 6(9): 1560 - 1573.
[Abstract] [Full Text] [PDF]

Home page
 GlycobiologyHome page
F. Fenaille, M. Le Mignon, C. Groseil, C. Ramon, S. Riande, L. Siret, and N. Bihoreau
Site-specific N-glycan characterization of human complement factor H
Glycobiology, September 1, 2007; 17(9): 932 - 944.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
Y. A. Daniel, C. Turner, R. M. Haynes, B. J. Hunt, and R. N. Dalton
Quantification of Hemoglobin A2 by Tandem Mass Spectrometry
Clin. Chem., August 1, 2007; 53(8): 1448 - 1454.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
R. L. Gundry and J. E. Van Eyk
Unraveling the Complexity of Circulating Forms of Brain Natriuretic Peptide
Clin. Chem., July 1, 2007; 53(7): 1181 - 1182.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
G. L. Hortin
A New Era in Protein Quantification in Clinical Laboratories: Application of Liquid Chromatography-Tandem Mass Spectrometry
Clin. Chem., April 1, 2007; 53(4): 543 - 544.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
O. P. Bondar, D. R. Barnidge, E. W. Klee, B. J. Davis, and G. G. Klee
LC-MS/MS Quantification of Zn-{alpha}2 Glycoprotein: A Potential Serum Biomarker for Prostate Cancer
Clin. Chem., April 1, 2007; 53(4): 673 - 678.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
M. G. Janech, J. R. Raymond, and J. M. Arthur
Proteomics in renal research
Am J Physiol Renal Physiol, February 1, 2007; 292(2): F501 - F512.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
W.-J. Qian, J. M. Jacobs, T. Liu, D. G. Camp II, and R. D. Smith
Advances and Challenges in Liquid Chromatography-Mass Spectrometry-based Proteomics Profiling for Clinical Applications
Mol. Cell. Proteomics, October 1, 2006; 5(10): 1727 - 1744.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
B. Domon and R. Aebersold
Challenges and Opportunities in Proteomics Data Analysis
Mol. Cell. Proteomics, October 1, 2006; 5(10): 1921 - 1926.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
G. L. Hortin, S. A. Jortani, J. C. Ritchie Jr, R. Valdes Jr, and D. W. Chan
Proteomics: A New Diagnostic Frontier
Clin. Chem., July 1, 2006; 52(7): 1218 - 1222.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2006 by the American Society for Biochemistry and Molecular Biology.
Advertisement
spacer
Advertisement
Advertisement