HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: M600170-MCP200v1 5/9/1638    most recent Submit a response Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Glossary Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ellmark, P. Articles by Borrebaeck, C. A. K. Search for Related Content PubMed PubMed Citation Articles by Ellmark, P. Articles by Borrebaeck, C. A. K. Social Bookmarking                      What's this?

Molecular & Cellular Proteomics 5:1638-1646, 2006.
© 2006 by The American Society for Biochemistry and Molecular Biology, Inc.

---

Research

Identification of Protein Expression Signatures Associated with Helicobacter pylori Infection and Gastric Adenocarcinoma Using Recombinant Antibody Microarrays^*

Peter Ellmark, Johan Ingvarsson, Anders Carlsson, B. Samuel Lundin, Christer Wingren and Carl A. K. Borrebaeck^,¶

From the Department of Immunotechnology, Lund University, BMC D13, SE-22184 Lund, Sweden and Institute of Biomedicine, Department of Microbiology and Immunology, Göteborg University, 40530 Göteborg, Sweden

Antibody microarray based technology is a powerful emerging tool in proteomics, target discovery, and differential analysis. Here, we report the first study where recombinant antibody fragments have been used to construct large scale antibody microarrays, composed of 127 different antibodies against mostly immunoregulatory antigens. The arrays were based on single framework recombinant antibody fragments (SinFabs) designed for high on-chip stability and functionality and were used for the analysis of malignant and normal stomach tissue samples from Helicobacter pylori-positive and -negative patients. Our results demonstrate that distinct tumor- as well as infection-associated protein expression signatures could be identified from these complex tissue proteomes, as well as biomarkers such as IL-9, IL-11, and MCP-4, previously not found in these diseases. In a longer perspective, this study may improve the understanding of H. pylori-induced stomach cancer and lead to development of improved diagnostics.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				C. Lausted, Z. Hu, and L. Hood Quantitative Serum Proteomics from Surface Plasmon Resonance Imaging Mol. Cell. Proteomics, December 1, 2008; 7(12): 2464 - 2474. [Abstract] [Full Text] [PDF]

				E. E. Forbes, K. Groschwitz, J. P. Abonia, E. B. Brandt, E. Cohen, C. Blanchard, R. Ahrens, L. Seidu, A. McKenzie, R. Strait, et al. IL-9- and mast cell-mediated intestinal permeability predisposes to oral antigen hypersensitivity J. Exp. Med., April 14, 2008; 205(4): 897 - 913. [Abstract] [Full Text] [PDF]